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![ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Recombinant-Protein-ELISA-NBP3-14666-img0001.jpg "ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.")
![ELISA: SARS-CoV-2 Spike Recombinant Protein [NBP3-14666] - SARS-CoV-2 Spike Recombinant Protein Binding Activity. SARS-CoV-2 Spike Recombinant Protein (Catalog # NBP3-14666) binds Recombinant Human ACE-2 His-tag (R&D Systems, Catalog # 933-ZN) in a functional ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Recombinant-Protein-ELISA-NBP3-14666-img0002.jpg)
![ELISA: SARS-CoV-2 SARS-CoV-2 Spike RBD [NBP3-11407] - Sample standard curve](http://images.novusbio.com/fullsize/SARS-CoV-2-SARS-CoV-2-Spike-RBD-ELISA-NBP3-11407-img0001.jpg "ELISA: SARS-CoV-2 SARS-CoV-2 Spike RBD [NBP3-11407] - Sample standard curve")
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-212024939162.jpg "ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BQ.1 variant, His tag (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) or goat anti-mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Indirect ELISA analysis was performed by coating the plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 Alpha variant; B.1.351 Beta variant; P.1 Gamma variant; B1.617.2 Delta variant; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (28.78-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-212024934276.jpg)
![Functional (Inhibition): SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Inhibition analysis of immobilized recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 ug/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (1000 ng/mL) binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (0.09-66.67 nM). Bound was detected by Goat Anti-Mouse IgG antibody (HRP) (1:10000).](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-2120249432894.jpg)
![ELISA: SARS-CoV-2 Spike RBD Antibody (HL1866) - Omicron Variant - Azide and BSA Free [NBP3-25671] - Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.4/ BA.5 Omicron variant; Omicron / XBB variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (5 ug/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD Omicron antibody [HL1866] (NBP3-25671) (1 ug/mL) Please notice that NBP3-25671 needs to be conjugated to HRP to function as the detection antibody when paired with HL1014. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25671_rabbit-sars-cov-2-spike-rbd-mab-hl1866-omicron-variant-azide-bsa-free-21202494089.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India [NBP3-14793] - SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14793, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7E3C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14793-img0004.jpg "ELISA: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India [NBP3-14793] - SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14793, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.")
![ELISA: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India [NBP3-14793] - ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14793, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7E3C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14793-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India [NBP3-14793] - ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT, Alpha variant, and Delta variant, 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, NBP3-14793, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R), but not WT peptide (681P) and Alpha variant spike S1 peptide (681H).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7E3C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14793-img0003.jpg)
![Immunohistochemistry: SARS-CoV-2 Spike Antibody (7E3C5) - Delta Variant, B.1.617.2, India - BSA Free [NBP3-14793] - Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue. Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody at 20 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-14793_mouse-sars-cov-2-spike-mab-7e3c5-delta-variant-b-1-617-2-india-2711202419261828.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - Validation of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike Antibody alpha variant (B.1.1.7) UK NBP3-11942, dilution: 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11942 can specifically detect omicron and alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7C11H11-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11942-img0004.jpg "ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - Validation of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike Antibody alpha variant (B.1.1.7) UK NBP3-11942, dilution: 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11942 can specifically detect omicron and alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.")
![Western Blot: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by WB.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7C11H11-Alpha-Variant-B-1-1-7-UK-Western-Blot-NBP3-11942-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7C11H11-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11942-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (7C11H11) - Alpha Variant, B.1.1.7, UK [NBP3-11942] - ELISA Validation of Alpha Variant Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11942, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11942) can specifically detect alpha variant spike S1 peptide (681H), but not WT peptide (681P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-7C11H11-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11942-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5G12G11-Gamma-Variant-P-1-Brazil-ELISA-NBP3-12856-img0003.jpg "ELISA: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.")
![Western Blot: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, NBP3-12856, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5G12G11-Gamma-Variant-P-1-Brazil-Western-Blot-NBP3-12856-img0004.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, NBP3-12856, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5G12G11-Gamma-Variant-P-1-Brazil-ELISA-NBP3-12856-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5G12G11) - Gamma Variant, P.1, Brazil [NBP3-12856] - ELISA Validation of Gamma Variant Spike S1 Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT and Gamma variant (P.1), 1 ug/mL, incubated at 4 C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, NBP3-12856, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody detects Gamma variant spike S1 peptide (26S), but not WT peptide (26P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5G12G11-Gamma-Variant-P-1-Brazil-ELISA-NBP3-12856-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody Alpha Variant (B.1.1.7) UK NBP3-11941, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11941 can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-9F7E4-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11941-img0004.jpg "ELISA: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4C overnight. Detection Antibody: SARS-CoV-2 Spike antibody Alpha Variant (B.1.1.7) UK NBP3-11941, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibody: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. NBP3-11941 can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants.")
![Western Blot: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - WB Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11941, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11941) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by WB.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-9F7E4-Alpha-Variant-B-1-1-7-UK-Western-Blot-NBP3-11941-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - ELISA Validation of Alpha Variant Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11941, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11941) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by ELISA.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-9F7E4-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11941-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (9F7E4) - Alpha Variant, B.1.1.7, UK [NBP3-11941] - ELISA Validation of Alpha Variant Spike Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11941, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11941) can specifically detect alpha variant spike S1 peptide (681H), but not WT peptide (681P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-9F7E4-Alpha-Variant-B-1-1-7-UK-ELISA-NBP3-11941-img0002.jpg)
![Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] - Sandwich ELISA for SARS-CoV-2 Spike S1 Matched Pair Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, NBP3-07065 and NBP3-26919. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (NBP3-07065, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein, and the anti-SARS-CoV-2 Spike S1 antibody (NBP3-26919, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S1-Antibody-SP185-Sandwich-ELISA-NBP3-07065-img0001.jpg "Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] - Sandwich ELISA for SARS-CoV-2 Spike S1 Matched Pair Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, NBP3-07065 and NBP3-26919. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (NBP3-07065, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein, and the anti-SARS-CoV-2 Spike S1 antibody (NBP3-26919, 1ug/ml) as the detection antibody. Secondary: Mouse anti-human IgG HRP conjugate at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.58 ng.")
![Sandwich ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) [NBP3-07065] - Sandwich ELISA for SARS-CoV-2 Spike S1 Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (NBP3-07065, 2ug/ml) as capture antibody, a Spike S1 recombinant protein as the binding protein, and an anti-SARS-CoV-2 Spike S1 antibody (1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-S1-Antibody-SP185-Sandwich-ELISA-NBP3-07065-img0002.jpg)
![Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free [NBP3-07065] - Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung.Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Spike antibody (3 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Spike protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-07065_rabbit-monoclonal-sars-cov-2-spike-s1-antibody-sp185-2711202419293820.jpg)
![ELISA: SARS-CoV-2 Spike S1 Antibody (SP185) - Azide and BSA Free [NBP3-07065] - Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7), SA variant (B.1.135) and Brazil (P.1). Dilution: 1-3000 ng/mL. Incubate at 4C overnight.Detection Antibodies: SARS-CoV-2 Spike S1 Antibody, 0.1 ug/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Spike S1 antibody cannot detect SA variant (B.1.135) and Brazil (P.1), both of which carry more mutations in RBD as compared to UK variant (B.1.1.7).](http://images.novusbio.com/fullsize/nbp3-07065_rabbit-monoclonal-sars-cov-2-spike-s1-antibody-sp185-271120241937754.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5H4C5) - Delta Variant, B.1.617.2, India [NBP3-14792] - SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14792, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5H4C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14792-img0001.jpg "ELISA: SARS-CoV-2 Spike Antibody (5H4C5) - Delta Variant, B.1.617.2, India [NBP3-14792] - SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14792, 1 ug/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr.")
![ELISA: SARS-CoV-2 Spike Antibody (5H4C5) - Delta Variant, B.1.617.2, India [NBP3-14792] - ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, NBP3-14792, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5H4C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14792-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody (5H4C5) - Delta Variant, B.1.617.2, India [NBP3-14792] - ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT, Alpha variant, and Delta variant, 1 ug/mL, incubated at 4C overnight. Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, NBP3-14792, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R), but not WT peptide (681P) and Alpha variant spike S1 peptide (681H).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody-5H4C5-Delta-Variant-B-1-617-2-India-ELISA-NBP3-14792-img0003.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Examination of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7) UK, beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK can specifically detect alpha variant and Mu spike S1 protein, but not WT and other tested variants.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11931-img0003.jpg "ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Examination of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7) UK, beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK can specifically detect alpha variant and Mu spike S1 protein, but not WT and other tested variants.")
![Western Blot: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Validation of UK Variant Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11931, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11931) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Alpha-Variant--B.1.1.7--UK-Western-Blot-NBP3-11931-img0002.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - ELISA Validation of UK Variant Spike Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and UK variant (B.1.1.7), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11931, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Immunogen region of antibody NBP3-11931 includes site 681P that was mutated in B.1.1.7. SARS-CoV-2 Spike antibody (NBP3-11931) can specifically detect UK variant spike S1 peptide (681H), but not WT peptide (681P).](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11931-img0001.jpg)
![ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Validation of SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) with Mutant and WT Spike S1 Proteins. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11931, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11931) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein.](http://images.novusbio.com/fullsize/SARS-CoV-2-Spike-Antibody---Alpha-Variant--B.1.1.7--UK-ELISA-NBP3-11931-img0004.jpg)