HBsAg ELISA Kit (Colorimetric)

• Applications: ELISA, NULL
• Suitable Sample Type: Plasma, Serum
• Sensitivity: 0.2 ng/mL

HBsAg ELISA Kit (Colorimetric) Summary

• Description: Hepatitis B surface antigen ELISA Kit is a sandwich enzyme immunoassay for the measurement of hepatitis B surface antigen.
• Sensitivity: 0.2 ng/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: S

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HBsAg ELISA Kit (Colorimetric)

• HBsAg
• HBV major surface antigen
• HBV surface antigen
• Hepatitis B Virus major surface antigen
• Hepatitis B Virus Surface Antigen
• Major surface antigen
• protein S

Background

Hepatitis B surface antigen ELISA Kit is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immuno-sorbent assay) based on the "sandwich principle". The solid phase of the microtiter plate is made of polystyrene wells coated with mouse monoclonal antibodies specific for HBsAg; whereas guinea pig polyclonal antibody purified by affinity chromatography is used to prepare the anti-HBs peroxidase (horseradish) conjugate in the liquid-phase. When a serum or plasma specimen containing HBsAg is added to the anti-HBs antibody-coated wells together with the peroxidase conjugated anti-HBs antibody and incubated, an antibody-HBsAg-antibody-peroxidase complex will form on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. A color develops in proportion to the amount of HBsAg bound to Anti-HBs. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HBsAg ELISA Kit (KA0286)(12)

Publications using KA0286

• Choi YM, Kim DH, Jang J, Kim BJ A hepatitis B virus-derived peptide combined with HBsAg exerts an anti-HBV effect in an HBV transgenic mouse model as a therapeutic vaccine Frontiers in immunology 2023-06-02 [PMID: 37334373]
• Edelblute C, Mangiamele C, Heller R Moderate heat-assisted gene electrotransfer for cutaneous delivery of a DNA vaccine against Hepatitis B virus Human gene therapy 2021-04-30 [PMID: 33926214] (ELISA)
• Xiaohe L, Zhiyi K, Dongli L et al. Combination of saikosaponin c and telbivudine synergistically enhances the anti-HBV activity. Inflamm Res. 2020-04-20 [PMID: 32313973]
• Kwan S, Jiao J, Qi J et al. Bile Acid Changes Associated With Liver Fibrosis and Steatosis in the Mexican-American Population of South Texas. Hepatol Commun. 2020-03-19 [PMID: 32258950]
• Sa-Ngiamsuntorn K, Thongsri P, Pewkliang Y et al. An Immortalized Hepatocyte-like Cell Line (imHC) Accommodated Complete Viral Lifecycle, Viral Persistence Form, cccDNA and Eventual Spreading of a Clinically-Isolated HBV. Viruses. 2019-10-16 [PMID: 31623162]
• Pan Y, Ke Z, Ye H et al. Saikosaponin C exerts anti-HBV effects by attenuating HNF1? and HNF4? expression to suppress HBV pgRNA synthesis. Inflamm Res. 2019-09-17 [PMID: 31531682]
• Kang J, Kim S, Park M et al. Ciclopirox inhibits Hepatitis B Virus secretion by blocking capsid assembly. Nat Commun. 2019-05-16 [PMID: 31097716]
• Zhang Z, He P, Zhou Y et al. Anti-HBV effect of interferon-thymosin ?1 recombinant proteins in transgenic Dunaliella salina in vitro and in vivo. Exp Ther Med. 2018-05-29 [PMID: 30112022]
• Yanli Zeng, Zixi Li, Jia Shang, Yi Kang Efficient delivery of HBV NLS siRNAs into HepG2.2.15 cells for HBV inhibition through novel recombinant preS1‑tP proteins. International journal of molecular medicine 2018-10-09 [PMID: 29786106]
• Siburian MD, Suriapranata IM, Wanandi SI. et al. Pre-S2 Start Codon Mutation of Hepatitis B Virus Subgenotype B3 Effects on NF-?B Expression and Activation in Huh7 Cell Lines. Viral Immunol 2018-03-19 [PMID: 29652648]
• Ahmad SM, Hossain MB, Monirujjaman M et al. Maternal zinc supplementation improves hepatitis B antibody responses in infants but decreases plasma zinc level. Eur J Nutr 2016-07-25 [PMID: 26208687]
• Wang Q, Luan W, Warren L et al. Serum hepatitis B surface antigen correlates with tissue covalently closed circular DNA in patients with hepatitis B-associated hepatocellular carcinoma. J Med Virol. 2015-07-14 [PMID: 26174662]

FAQs for HBsAg ELISA Kit (KA0286). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: S