HBsAg ELISA Kit (Colorimetric) Summary
Hepatitis B surface antigen ELISA Kit is a sandwich enzyme immunoassay for the measurement of hepatitis B surface antigen.
ELISA Kit (Colorimetric)
Useful for qualification.
Packaging, Storage & Formulations
- HB Negative Control
- HBsAg Positive Control
- Conc. Washing Solution D (20X)
- Anti-HBs Peroxidase Solution
- Anti-HBs Plate
- 2 N sulfuric acid
- TMB Substrate Solution A
- TMB Substrate Solution B
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for HBsAg ELISA Kit (Colorimetric)
- HBV major surface antigen
- HBV surface antigen
- Hepatitis B Virus major surface antigen
- Hepatitis B Virus Surface Antigen
- Major surface antigen
- protein S
Hepatitis B surface antigen ELISA Kit is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immuno-sorbent assay) based on the "sandwich principle". The solid phase of the microtiter plate is made of polystyrene wells coated with mouse monoclonal antibodies specific for HBsAg; whereas guinea pig polyclonal antibody purified by affinity chromatography is used to prepare the anti-HBs peroxidase (horseradish) conjugate in the liquid-phase. When a serum or plasma specimen containing HBsAg is added to the anti-HBs antibody-coated wells together with the peroxidase conjugated anti-HBs antibody and incubated, an antibody-HBsAg-antibody-peroxidase complex will form on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. A color develops in proportion to the amount of HBsAg bound to Anti-HBs. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
Publications for HBsAg ELISA Kit (KA0286)(2)
Showing Publications 1 - 2
Reviews for HBsAg ELISA Kit (KA0286) (0)
There are no reviews for HBsAg ELISA Kit (KA0286).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for HBsAg ELISA Kit (KA0286) (0)
Additional HBsAg Products
Bioinformatics Tool for HBsAg ELISA Kit (KA0286)
Discover related pathways, diseases and genes to HBsAg ELISA Kit (KA0286). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on HBsAg