HBsAg ELISA Kit [HRP] Summary
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
Hepatitis B surface antigen ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of hepatitis B surface antigen.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for HBsAg ELISA Kit [HRP]
- HBV major surface antigen
- HBV surface antigen
- Hepatitis B Virus major surface antigen
- Hepatitis B Virus Surface Antigen
- Major surface antigen
Hepatitis B surface antigen ELISA Kit is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immuno-sorbent assay) based on the "sandwich principle". The solid phase of the microtiter plate is made of polystyrene wells coated with mouse monoclonal antibodies specific for HBsAg; whereas guinea pig polyclonal antibody purified by affinity chromatography is used to prepare the anti-HBs peroxidase (horseradish) conjugate in the liquid-phase. When a serum or plasma specimen containing HBsAg is added to the anti-HBs antibody-coated wells together with the peroxidase conjugated anti-HBs antibody and incubated, an antibody-HBsAg-antibody-peroxidase complex will form on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. A color develops in proportion to the amount of HBsAg bound to Anti-HBs. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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