HBsAg ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA
Suitable Sample Type
Plasma, Serum
Sensitivity
0.125 IU/mL

Order Details

HBsAg ELISA Kit (Colorimetric) Summary

Description
Hepatitis B surface antigen ELISA Kit is a sandwich enzyme immunoassay for the measurement of hepatitis B surface antigen.
Sensitivity
0.125 IU/mL
Assay Type
Sandwich ELISA
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HBVgp2

Applications/Dilutions

Application Notes
Useful for qualification.
Publications
Read Publications using KA0286.

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. HB Negative Control
  2. HBsAg Positive Control
  3. Conc. Washing Solution D (20X)
  4. Anti-HBs Peroxidase Solution
  5. Anti-HBs Plate
  6. 2 N sulfuric acid
  7. TMB Substrate Solution A
  8. TMB Substrate Solution B

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HBsAg ELISA Kit (Colorimetric)

  • HBsAg
  • HBV major surface antigen
  • HBV surface antigen
  • Hepatitis B Virus major surface antigen
  • Hepatitis B Virus Surface Antigen
  • Major surface antigen
  • protein S

Background

Hepatitis B surface antigen ELISA Kit is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immuno-sorbent assay) based on the "sandwich principle". The solid phase of the microtiter plate is made of polystyrene wells coated with mouse monoclonal antibodies specific for HBsAg; whereas guinea pig polyclonal antibody purified by affinity chromatography is used to prepare the anti-HBs peroxidase (horseradish) conjugate in the liquid-phase. When a serum or plasma specimen containing HBsAg is added to the anti-HBs antibody-coated wells together with the peroxidase conjugated anti-HBs antibody and incubated, an antibody-HBsAg-antibody-peroxidase complex will form on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. A color develops in proportion to the amount of HBsAg bound to Anti-HBs. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HBsAg ELISA Kit (KA0286)(2)

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Bioinformatics

Gene Symbol HBVgp2