HBsAg ELISA Kit (Colorimetric)


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Product Details

Applications ELISA, QFN
Suitable Sample Type
Plasma, Serum
0.2 ng/mL

Order Details

HBsAg ELISA Kit (Colorimetric) Summary

Hepatitis B surface antigen ELISA Kit is a sandwich enzyme immunoassay for the measurement of hepatitis B surface antigen.
0.2 ng/mL
Assay Type
Sandwich ELISA
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)


Application Notes
Useful for qualification.
Read Publications using KA0286.

Packaging, Storage & Formulations

Store at 4C.

Kit Components

  1. HB Negative Control
  2. HBsAg Positive Control
  3. Anti-HBs Peroxidase Solution
  4. Anti-HBs Plate
  5. 2 N sulfuric acid
  6. Conc. Washing Solution D (20X)
  7. TMB Substrate Solution A
  8. TMB Substrate Solution B


This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HBsAg ELISA Kit (Colorimetric)

  • HBsAg
  • HBV major surface antigen
  • HBV surface antigen
  • Hepatitis B Virus major surface antigen
  • Hepatitis B Virus Surface Antigen
  • Major surface antigen
  • protein S


Hepatitis B surface antigen ELISA Kit is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immuno-sorbent assay) based on the "sandwich principle". The solid phase of the microtiter plate is made of polystyrene wells coated with mouse monoclonal antibodies specific for HBsAg; whereas guinea pig polyclonal antibody purified by affinity chromatography is used to prepare the anti-HBs peroxidase (horseradish) conjugate in the liquid-phase. When a serum or plasma specimen containing HBsAg is added to the anti-HBs antibody-coated wells together with the peroxidase conjugated anti-HBs antibody and incubated, an antibody-HBsAg-antibody-peroxidase complex will form on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. A color develops in proportion to the amount of HBsAg bound to Anti-HBs. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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