Reactivity | VSpecies Glossary |
Applications | ELISA, ICC/IF, SPR |
Clone | CR3022 |
Clonality | Monoclonal |
Host | Human |
Conjugate | Unconjugated |
Concentration | 1 mg/ml |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV. |
Specificity | This antibody binds to both SARS-CoV and SARS-CoV-2 with high affinity (PMID: 16796401 & 32065055). It binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318-510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" confromation of the spike protein (Joyce et al. 2020). |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Human |
Gene | S |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
|
Application Notes | The initial characterization of the binding of this antibody was performed by ELISA and indicates potential for the development of diagnostic assays, as both virus-capture assays, or as controls in serological assays measuring immune-responses to virus exposure. Human IgG1, IgG3, IgM and IgA isotypes are available to mimic antibody responses seen in COVID19 (Amanat et al. 2020). Human IgG2 is available to assess its yet unknown role in the response to SARS-CoV-2. The original human IgG1 version of the antibody works synergistically in combination with another non-competing SARS antibody and is a potential candidate for passive immune prophylaxis of SARS-CoV infection (Meulen et al., 2006). The original antibody (human IgG1) was also reported to bind the 2019-nCoV RBD (KD of 6.3 nM). This antibody has been attributed a potential to be developed as a therapeutic agent, alone or in combination with other neutralizing antibodies for treatment of 2019-nCoV infections (Tian et al., 2020). |
|
Publications |
|
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.02% Proclin 300 |
Concentration | 1 mg/ml |
Purity | Protein A purified |
Publications using NBP2-90980 | Applications | Species |
---|---|---|
Kaneko T, Esmail S, Voss C et al. System-wide hematopoietic and immune signaling aberrations in COVID-19 revealed by deep proteome and phosphoproteome analysis Research Square Feb 10 2021 (MiAr) | MiAr | |
Esmail S, Knauer M, Abdoh H et al. Rapid and accurate agglutination-based testing for SARS-CoV-2 antibodies Cell Reports Methods May 1 2021 | ||
Staufer O, Gupta K, Hernandez BUcher JE et al. Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoprotein Nature communications Feb 14 2022 [PMID: 35165285] (SARS-CoV-2) | SARS-CoV-2 | |
Cai Q, Mu J, Lei Y et al. Simultaneous detection of the spike and nucleocapsid proteins from SARS-CoV-2 based on ultrasensitive single molecule assays Anal Bioanal Chem May 31 2021 [PMID: 34057558] |
Secondary Antibodies |
Isotype Controls |
Research Areas for SARS-CoV-2 Spike Antibody (NBP2-90980)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.