| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43110-0.1mg | |||
| Azide and BSA Free | NBP2-34497 | |||
| BSA Free | NBP1-43110-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43107-0.025mg | |||
| Azide and BSA Free | NBP2-34498 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77277-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43110-0.5mg | |||
| BSA Free | NBP1-43110-0.1mg | |||
| BSA Free | NBP1-43110-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75519-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48285 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB10862-SP | |||
![Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis of PE conjugate of NBP1-43110. The J43 antibody has been tested by flow cytometric mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be de](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0009.jpg "Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis of PE conjugate of NBP1-43110. The J43 antibody has been tested by flow cytometric mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be de")
![Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0002.jpg)
![Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Biotin conjugate of NBP1-43110. Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0003.jpg)
![Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE conjugate. This can be used at less than or equal to 0.5 ug per test. Cell number should be](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0007.jpg)
![Flow Cytometry: PD-1 Antibody (J43) [NBP1-43110] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Flow-Cytometry-NBP1-43110-img0008.jpg)
![Flow Cytometry: PD-1 Antibody (J116) [NBP1-43107] - Staining of human PD-1 transfected cells with anti-human Anti-Human CD279 (PD-1) FITC (left)or PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J116-Flow-Cytometry-NBP1-43107-img0003.jpg "Flow Cytometry: PD-1 Antibody (J116) [NBP1-43107] - Staining of human PD-1 transfected cells with anti-human Anti-Human CD279 (PD-1) FITC (left)or PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.")
![Western Blot: PD-1 Antibody [NBP1-77277] - THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/PD-1-Antibody-Western-Blot-NBP1-77277-img0002.jpg "Western Blot: PD-1 Antibody [NBP1-77277] - THP-1 cell lysate with PD-1 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: PD-1 Antibody - BSA Free [NBP1-77277] - Immunocytochemistry of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77277_rabbit-polyclonal-pd-1-antibody-271120242013292.gif)
![Immunocytochemistry/ Immunofluorescence: PD-1 Antibody - BSA Free [NBP1-77277] - Immunofluorescence of PD-1 in PD-1-transfected HEK293 cells with PD-1 antibody at 20 ug/ml.Green: PD-1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77277_rabbit-polyclonal-pd-1-antibody-2711202420115535.gif)
![Immunohistochemistry: PD-1 Antibody - BSA Free [NBP1-77277] - Immunohistochemistry of PD-1 in human brain tissue with PD-1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77277_rabbit-polyclonal-pd-1-antibody-271120241928869.jpg)
![Immunocytochemistry/Immunofluorescence: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-34497-img0004.jpg "Immunocytochemistry/Immunofluorescence: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.")
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0005.jpg)
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0001.jpg)
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0003.jpg)
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE conjugate. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0002.jpg)
![Western Blot: PD-1 Antibody (12A7D7) - BSA Free [NBP1-75519] - Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 u/mL.](http://images.novusbio.com/fullsize/nbp1-75519_mouse-monoclonal-pd-1-antibody-12a7d7-2711202420195374.jpg "Western Blot: PD-1 Antibody (12A7D7) - BSA Free [NBP1-75519] - Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 u/mL.")
![Immunohistochemistry: PD-1 Antibody (12A7D7) - BSA Free [NBP1-75519] - Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-75519_mouse-monoclonal-pd-1-antibody-12a7d7-2711202419261863.jpg)
![Immunohistochemistry: PD-1 Antibody (12A7D7) - BSA Free [NBP1-75519] - Immunohistochemistry of PD-1 in mouse thymus tissue with PD-1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-75519_mouse-monoclonal-pd-1-antibody-12a7d7-271120241923636.gif)
![Immunohistochemistry-Paraffin: PD-1 Antibody (PDCD1/922) - Azide and BSA Free [NBP2-47805] - Human Tonsil stained with PD1 (CD279) Monoclonal Antibody (PDCD1/922).](http://images.novusbio.com/fullsize/PD-1-Antibody-PDCD1-922-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47805-img0001.jpg "Immunohistochemistry-Paraffin: PD-1 Antibody (PDCD1/922) - Azide and BSA Free [NBP2-47805] - Human Tonsil stained with PD1 (CD279) Monoclonal Antibody (PDCD1/922).")
![Immunohistochemistry-Paraffin: PD-1 Antibody (PDCD1/922) - Azide and BSA Free [NBP2-47805] - Human Tonsil stained with PD1 (CD279) Monoclonal Antibody (PDCD1/922).](http://images.novusbio.com/fullsize/PD-1-Antibody-PDCD1-922-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47805-img0002.jpg)
![N/A PD-1 [Biotin]](http://images.novusbio.com/fullsize2/DATA_PD1_DY1021_ELISA_1550.jpg "N/A PD-1 [Biotin]")
![Western Blot: PD-1 Antibody (4F12) [NBP1-04328] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate, Lane 2.: Raji cell lysate](http://images.novusbio.com/fullsize/PD-1-Antibody-4F12-Western-Blot-NBP1-04328-img0005.jpg "Western Blot: PD-1 Antibody (4F12) [NBP1-04328] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate, Lane 2.: Raji cell lysate")
![Immunocytochemistry/Immunofluorescence: PD-1 Antibody (4F12) [NBP1-04328] - HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PD-1-Antibody-4F12-Immunocytochemistry-Immunofluorescence-NBP1-04328-img0004.jpg)
![Flow Cytometry: PD-1 Antibody (4F12) [NBP1-04328] - Flow cytometry analysis of PDCD1 in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.](http://images.novusbio.com/fullsize/PD-1-Antibody-4F12-Flow-Cytometry-NBP1-04328-img0003.jpg)
![Western Blot: PD1 Peptide [NBP1-77276PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.](http://images.novusbio.com/fullsize/PD1-Peptide-Western-Blot-NBP1-77276PEP-img0001.jpg "Western Blot: PD1 Peptide [NBP1-77276PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.")