| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73226 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38499-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73228 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97309SS | |||
![Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Western-Blot-NBP2-02082-img0025.jpg "Western Blot: PADI4 Antibody (4H5) [NBP2-02082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAD4.")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PAD4.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-02082-img0016.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI4H5) [NBP2-02082] - Staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0027.jpg)
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Jurkat cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0014.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0017.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0018.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0019.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0020.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0021.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human breast tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0022.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human pancreas tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0023.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (4H5) [NBP2-02082] - Staining of paraffin-embedded Human prostate tissue using anti-PAD4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-02082-img0024.jpg)
![Flow Cytometry: PADI4 Antibody (4H5) [NBP2-02082] - Analysis of Hela cells, using anti-PAD4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PADI4-Antibody-4H5-Flow-Cytometry-NBP2-02082-img0015.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining in human bone marrow and kidney tissues using anti-PADI4 antibody. Corresponding PADI4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining in human bone marrow and kidney tissues using anti-PADI4 antibody. Corresponding PADI4 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP2-38499 (A) shows similar protein distribution across tissues to independent antibody NBP1-87353 (B).](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0012.jpg)
![Western Blot: PADI4 Antibody [NBP2-38499] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/PADI4-Antibody-Western-Blot-NBP2-38499-img0006.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0003.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0004.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human liver.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0008.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human testis.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0009.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human spleen.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0010.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow using Anti-PADI4 antibody NBP2-38499.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0011.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Analysis in human bone marrow and kidney tissues. Corresponding PADI4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Analysis in human bone marrow and kidney tissues. Corresponding PADI4 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP1-87353 (A) shows similar protein distribution across tissues to independent antibody NBP2-38499 (B).](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0019.jpg)
![Western Blot: PADI4 Antibody [NBP1-87353] - Analysis in control (vector only transfected HEK293T lysate) and pADI4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PADI4-Antibody-Western-Blot-NBP1-87353-img0010.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human testis shows no nuclear positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0023.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0018.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human kidney shows no nuclear positivity as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0020.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0021.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP1-87353] - Staining of human spleen shows strong nuclear positivity in cells in red pulp.](http://images.novusbio.com/fullsize/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP1-87353-img0022.jpg)
![Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Western-Blot-NBP2-46310-img0008.jpg "Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0007.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0001.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0002.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0003.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Human Ovary tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0004.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0005.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0006.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Western-Blot-H00023569-M01-img0001.jpg "Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (4D8) [H00023569-M01] - Analysis of monoclonal antibody to PADI4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Immunocytochemistry-Immunofluorescence-H00023569-M01-img0004.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Detection of enzymatically active peptidyl arginine deiminase (PAD) in the synovial fluid (SF) of patients with rheumatoid arthritis (RA). A, Immunoblotting of albumin‐depleted SF samples from 8 RA patients shows presence of neutrophil elastase (NE), PAD4, & PAD2. B, PAD activity in the SF of patients with RA (n = 12) was significantly higher than that in the SF of patients with osteoarthritis (OA) (n = 9). C, PAD activity was observed in the OA SF & RA SF both at physiologic calcium concentrations & at higher calcium concentrations (5 mM). In B & C, symbols represent individual patients; horizontal bars show the median. D, PAD activity is significantly correlated with DNA levels in the SF of RA patients (n = 22). Data were analyzed using Spearman's rho test for correlation. ∗ = P < 0.05; ∗∗ = P < 0.01, by Mann‐Whitney U test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415482220.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Release of peptidyl arginine deiminases (PADs) into the supernatant during NETosis, & detection of enzymatically active PADs. A, DNA release in peripheral blood neutrophils from healthy donors that were left unstimulated or stimulated with 25 mM phorbol myristate acetate (PMA) was assessed for up to 240 minutes after stimulation. Bars show the mean ± SD of 8 samples per group. B, For isolation of neutrophil extracellular traps, supernatants (SN) of unstimulated (unst.) or stimulated (stim.) neutrophils were collected. Subsequently, the cells were washed 3 times with RPMI medium (W1–W3), & stimulated cells were incubated in the absence or presence of DNase I; unstimulated cells treated with DNase I served as a control. Bars show the mean ± SD DNA concentration (conc) in 7 samples per group. C, Proteins (PAD2, PAD4, & neutrophil elastase [NE]) were precipitated from the same supernatants of unstimulated or stimulated cells as described in B & analyzed by Western blotting. Citrullinated proteins (citr.prot.) were detected using chemical modification & anti–modified citrulline antibody. PAD2 & PAD4 antibodies were tested for cross‐reactivity using human (hu) recombinant PAD4 (rPAD4) (250 ng) & human skeletal muscle tissue lysate (15 μg). One representative blot of 4 independent experiments is shown for each group. D, PAD activity in supernatants of unstimulated & stimulated cells was compared. Symbols represent individual donors (n = 10); bars show the median. ∗ = P < 0.05; ∗∗ = P < 0.01, by Wilcoxon's matched pairs signed rank test. AFU = arbitrary fluorescence units; NS = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415485135.jpg)
![Western Blot: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Western-Blot-NBP2-73228-img0001.jpg "Western Blot: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.")
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0005.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0002.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0003.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0004.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) - Azide and BSA Free [NBP2-73228] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-OTI5C10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73228-img0006.jpg)
![Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.](http://images.novusbio.com/fullsize/PAD4-Antibody-Immunohistochemistry-NBP1-97309-img0001.jpg "Immunohistochemistry: PAD4 Antibody [NBP1-97309] - PAD4 antibody was tested in mouse liver using DAB with hematoxylin counterstain. The image on the right has been blocked with the immunizing peptide.")
![Western Blot: PADI4 Antibody (PSH01-33) [NBP3-32707] - Western blot analysis of PADI4 on different lysates with Rabbit anti-PADI4 antibody (NBP3-32707) at 1/1,000 dilution. Lane 1: 293T overexpress PADI4 whole cell lysate Lane 2: 293T whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32707) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32707_rabbit-padi4-mab-psh01-33-278202415295544.jpg "Western Blot: PADI4 Antibody (PSH01-33) [NBP3-32707] - Western blot analysis of PADI4 on different lysates with Rabbit anti-PADI4 antibody (NBP3-32707) at 1/1,000 dilution. Lane 1: 293T overexpress PADI4 whole cell lysate Lane 2: 293T whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32707) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PADI4 Antibody (PSH01-33) [NBP3-32707] - Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PADI4 antibody (NBP3-32707) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32707) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32707_rabbit-padi4-mab-psh01-33-298202414161870.jpg)
![Western Blot: PADI4 Antibody (C17) [NBP3-41983] - Sample: Human Milk Primary Ab: 2ug/ml NBP3-41983; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41983_mouse-padi4-mab-c17-612202421351297.jpg "Western Blot: PADI4 Antibody (C17) [NBP3-41983] - Sample: Human Milk Primary Ab: 2ug/ml NBP3-41983; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: PADI4 Antibody (C17) [NBP3-41983] - Sample: Human Saliva Primary Ab: 1ug/ml NBP3-41983; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41983_mouse-padi4-mab-c17-612202421175984.jpg)
![Western Blot: PADI4 Antibody (C17) [NBP3-41983] - Sample: Human SalivaPrimary Ab: 1ug/ml NBP3-41983; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41983_mouse-padi4-mab-c17-612202421351282.jpg)
![Western Blot: PADI4 Antibody [NBP3-18652] - Figure 2. Western blot analysis using the antibody directed against PADI4 Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with the antibody against PADI4 , diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/PADI4-Antibody-Western-Blot-NBP3-18652-img0001.jpg "Western Blot: PADI4 Antibody [NBP3-18652] - Figure 2. Western blot analysis using the antibody directed against PADI4 Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 ug) with the antibody against PADI4 , diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.")
![ELISA: PADI4 Antibody [NBP3-18652] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against PADI4 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.](http://images.novusbio.com/fullsize/PADI4 Antibody-ELISA-NBP3-18652-img0002.jpg)