AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_lightbox">

AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." alt="Raji cells were stained with Goat Anti-Human IL‑16 C-terminal Peptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-316-PB, filled histogram) or isotype control antibody (Catalog #
AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_thumb" />
HAF017). Specific bands were detected for IL-16 at approximately 14 kDa (active) and 45-55 kDa (precursor), as indicated. This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">

HAF017). Specific bands were detected for IL-16 at approximately 14 kDa (active) and 45-55 kDa (precursor), as indicated. This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for
Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for
Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
316-IL) was serially diluted and captured by Mouse Anti-Human IL‑16 Monoclonal Antibody (Catalog #
MAB316) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human IL‑16 C-terminal Peptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-316-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

316-IL) was serially diluted and captured by Mouse Anti-Human IL‑16 Monoclonal Antibody (Catalog #
MAB316) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human IL‑16 C-terminal Peptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-316-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />