IGF-II/IGF2: Novus Biologicals

292-G2

Recombinant Human IGF-II/IGF2 Protein, CF

(51 Publications)

50 μg

292-G2

1 μg/lane of Recombinant Human IGF-II/IGF2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	292-G2-250

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792-MG

Recombinant Mouse IGF-II/IGF2 Protein, CF

(28 Publications)

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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AF-292-NA

IGF-II/IGF2 Antibody [Unconjugated]

(19 Publications)

250 μg

AF-292-NA

292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-292-NA). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox">

Recombinant Human IGF-II/IGF2 (Catalog # <a class=

292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-292-NA). The ND₅₀ is typically 0.2-0.8 µg/mL." alt="Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-292-NA). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />

CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

IGF-II/IGF2 was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 5 µg/mL Goat Anti-Human IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-292-NA) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Clinical relevance of tumour IGF2 and stromal VEGF axis.(a) Western blot showing expression of IGF2 in ESCC (T) and matched non-tumour specimens (N), as well as expression of VEGF and alpha -SMA in CAFs and matched NEF from 11 ESCC patients. (b) Graphs showing positive correlation between IGF2 expression in oesophageal tissue and expressions of VEGF (left panel) and alpha -SMA (right panel) in fibroblasts, respectively, in 11 pairs of ESCC and adjacent normal tissues. Correlation was assessed using Pearson's rank correlation coefficient. (c) Comparison of serum IGF2 (left panel) and VEGF (middle panel) levels between healthy individuals (n=50) and ESCC patients (n=100); the data were pooled and a positive correlation was found between serum VEGF and IGF2 levels (n=150) (right panel) using unpaired t-test. (d) Gene expressions were further divided into high and low levels using median expression level as the cut-off point for survival analyses. Kaplan–Meier curves comparing survival outcome of ESCC patients (n=100) with high and low serum IGF2 levels (left panel), high and low serum VEGF levels (middle panel), and IGF2 High/VEGF High and IGF2 Low/VEGF Low levels (right panel); statistical significance was calculated by log-rank test. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/ncomms14399), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-292-NAU-100UG
Alexa Fluor 405	AF-292-NAV-100UG
Alexa Fluor 488	AF-292-NAG-100UG
Alexa Fluor 532	AF-292-NAX-100UG
Alexa Fluor 594	AF-292-NAT-100UG
Alexa Fluor 647	AF-292-NAR-100UG
Alexa Fluor 700	AF-292-NAN-100UG
Alexa Fluor 750	AF-292-NAS-100UG
Alexa Fluor Plus 405	AF-292-NAAFP405
Alexa Fluor Plus 488	AF-292-NAAFP488
Alexa Fluor Plus 555	AF-292-NAAFP555
Alexa Fluor Plus 594	AF-292-NAAFP594
Alexa Fluor Plus 647	AF-292-NAAFP647
Alexa Fluor Plus 680	AF-292-NAAFP680
Allophycocyanin	AF-292-NAA
Biotin	BAF292
CoraFluor 1	AF-292-NACL1
DyLight 350	AF-292-NAD
DyLight 405	AF-292-NAE
DyLight 488	AF-292-NAK
DyLight 550	AF-292-NAL
DyLight 594	AF-292-NAM
DyLight 650	AF-292-NAW
DyLight 680	AF-292-NAY
DyLight 755	AF-292-NAZ
FITC	AF-292-NAF
HRP	AF-292-NAH
Janelia Fluor 525	AF-292-NAJF525
Janelia Fluor 549	AF-292-NAI
Janelia Fluor 646	AF-292-NAJ
Janelia Fluor 669	AF-292-NAJF669
PE	AF-292-NAP
PerCP	AF-292-NAC
mFluor Violet 450 SE	AF-292-NAMFV450
mFluor Violet 500 SE	AF-292-NAMFV500
mFluor Violet 610 SE	AF-292-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	BAF292
Carrier Free	AF-292-SP

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AF792

IGF-II/IGF2 Antibody [Unconjugated]

(12 Publications)

100 μg

AF792

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

IGF-II/IGF2 was detected in immersion fixed frozen sections of mouse embryo (E13-17) using Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="IGF-II/IGF2 was detected in immersion fixed frozen sections of mouse embryo (E13-17) using Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

792-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-II/IGF2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_lightbox">

Recombinant Mouse IGF-II/IGF2 (Catalog # <a class=

792-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-II/IGF2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_thumb" />

792-MG) was serially diluted and captured by Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

792-MG) was serially diluted and captured by Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF792U-100UG
Alexa Fluor 405	AF792V-100UG
Alexa Fluor 488	AF792G-100UG
Alexa Fluor 532	AF792X-100UG
Alexa Fluor 594	AF792T-100UG
Alexa Fluor 647	AF792R-100UG
Alexa Fluor 700	AF792N-100UG
Alexa Fluor 750	AF792S-100UG
Alexa Fluor Plus 405	AF792AFP405-100UG
Alexa Fluor Plus 488	AF792AFP488-100UG
Alexa Fluor Plus 555	AF792AFP555-100UG
Alexa Fluor Plus 594	AF792AFP594-100UG
Alexa Fluor Plus 647	AF792AFP647-100UG
Alexa Fluor Plus 680	AF792AFP680-100UG
Allophycocyanin	AF792A
Biotin	BAF792
CoraFluor 1	AF792CL1
DyLight 350	AF792D
DyLight 405	AF792E
DyLight 488	AF792K
DyLight 550	AF792L
DyLight 594	AF792M
DyLight 650	AF792W
DyLight 680	AF792Y
DyLight 755	AF792Z
FITC	AF792F
HRP	AF792H
Janelia Fluor 525	AF792JF525
Janelia Fluor 549	AF792I
Janelia Fluor 646	AF792J
Janelia Fluor 669	AF792JF669
PE	AF792P
PerCP	AF792C
mFluor Violet 450 SE	AF792MFV450
mFluor Violet 500 SE	AF792MFV500
mFluor Violet 610 SE	AF792MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	BAF792
Carrier Free	AF792-SP

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NBP2-37462

IGF-II/IGF2 Antibody (8H1) - BSA Free

(1 Publication)

0.1 ml

NBP2-37462

Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of paraffin-embedded bladder cancer tissues using IGF-II/IGF2 (8H1)mouse mAb with DAB staining.

Flow Cytometry: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of HepG2 cells using IGF2 mouse mAb (green) and negative control (red).

Western Blot: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis using IGF2 mAb against human IGF2 recombinant protein. (Expected MW is 43.1 kDa)

Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Analysis of ovarian cancer tissues using IGF2 mouse mAb with DAB staining.

ELISA: IGF-II/IGF2 Antibody (8H1) [NBP2-37462] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37462-0.025ml

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DY792

Mouse IGF-II/IGF2 DuoSet ELISA, 15 Plate

(11 Publications)

1 Kit (for 15 Plates)

DY792

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB292

IGF-II/IGF2 Antibody (75015) [Unconjugated]

(9 Publications)

100 μg

MAB292

292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB292). The ND₅₀ is typically 2-12 µg/mL." class="big_lightbox">

292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB292). The ND₅₀ is typically 2-12 µg/mL." alt="Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-II/IGF2 (14 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB292). The ND₅₀ is typically 2-12 µg/mL." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB292U-100UG
Alexa Fluor 405	FAB292V-100UG
Alexa Fluor 488	FAB292G-100UG
Alexa Fluor 532	FAB292X-100UG
Alexa Fluor 594	FAB292T-100UG
Alexa Fluor 647	FAB292R-100UG
Alexa Fluor 700	FAB292N-100UG
Alexa Fluor 750	FAB292S-100UG
Alexa Fluor Plus 405	FAB292AFP405-100UG
Alexa Fluor Plus 488	FAB292AFP488-100UG
Alexa Fluor Plus 555	FAB292AFP555-100UG
Alexa Fluor Plus 594	FAB292AFP594-100UG
Alexa Fluor Plus 647	FAB292AFP647-100UG
Alexa Fluor Plus 680	FAB292AFP680-100UG
Allophycocyanin	FAB292A
Biotin	FAB292B
CoraFluor 1	FAB292CL1
DyLight 350	FAB292D
DyLight 405	FAB292E
DyLight 488	FAB292K
DyLight 550	FAB292L
DyLight 594	FAB292M
DyLight 650	FAB292W
DyLight 680	FAB292Y
DyLight 755	FAB292Z
FITC	FAB292F
HRP	FAB292H
Janelia Fluor 525	FAB292JF525
Janelia Fluor 549	FAB292I
Janelia Fluor 646	FAB292J
Janelia Fluor 669	FAB292JF669
PE	FAB292P
PerCP	FAB292C
mFluor Violet 450 SE	FAB292MFV450
mFluor Violet 500 SE	FAB292MFV500
mFluor Violet 610 SE	FAB292MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB292-SP
Carrier Free	MAB292-500

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MAB792

IGF-II/IGF2 Antibody (122404) [Unconjugated]

(4 Publications)

100 μg

MAB792

791-MG), Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) and Recombinant Mouse IGF-I/IGF1 (Catalog # 791-MG). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IGF-II/IGF2 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

Western blot shows 50 ng of Recombinant Mouse IGF-I/IGF1 (Catalog # <a class=

791-MG), Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) and Recombinant Mouse IGF-I/IGF1 (Catalog # 791-MG). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IGF-II/IGF2 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 50 ng of Recombinant Mouse IGF-I/IGF1 (Catalog # 791-MG), Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) and Recombinant Mouse IGF-I/IGF1 (Catalog # 791-MG). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IGF-II/IGF2 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

792-MG) was serially diluted and captured by Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

792-MG) was serially diluted and captured by Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-II/IGF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF792) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB792U-100UG
Alexa Fluor 405	FAB792V-100UG
Alexa Fluor 488	FAB792G-100UG
Alexa Fluor 532	FAB792X-100UG
Alexa Fluor 594	FAB792T-100UG
Alexa Fluor 647	FAB792R-100UG
Alexa Fluor 700	FAB792N-100UG
Alexa Fluor 750	FAB792S-100UG
Alexa Fluor Plus 405	FAB792AFP405-100UG
Alexa Fluor Plus 488	FAB792AFP488-100UG
Alexa Fluor Plus 555	FAB792AFP555-100UG
Alexa Fluor Plus 594	FAB792AFP594-100UG
Alexa Fluor Plus 647	FAB792AFP647-100UG
Alexa Fluor Plus 680	FAB792AFP680-100UG
Allophycocyanin	FAB792A
Biotin	FAB792B
CoraFluor 1	FAB792CL1
DyLight 350	FAB792D
DyLight 405	FAB792E
DyLight 488	FAB792K
DyLight 550	FAB792L
DyLight 594	FAB792M
DyLight 650	FAB792W
DyLight 680	FAB792Y
DyLight 755	FAB792Z
FITC	FAB792F
HRP	FAB792H
Janelia Fluor 525	FAB792JF525
Janelia Fluor 549	FAB792I
Janelia Fluor 646	FAB792J
Janelia Fluor 669	FAB792JF669
PE	FAB792P
PerCP	FAB792C
mFluor Violet 450 SE	FAB792MFV450
mFluor Violet 500 SE	FAB792MFV500
mFluor Violet 610 SE	FAB792MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB792-SP
Carrier Free	MAB792-500

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MAB2921

IGF-II/IGF2 Antibody (75014) [Unconjugated]

(1 Publication)

100 μg

MAB2921

HAF018). A specific band was detected for IGF-II/IGF2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human liver tissue, Huh-7 human hepatoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB2921) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for IGF-II/IGF2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue, Huh-7 human hepatoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB2921) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-II/IGF2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

The MYOD1-mediated IGF2 gene expressions and cell viability. (A,B) mRNA and protein expression levels of MYOD1 in CEFs overexpressing distinct siRNAs. (C) Proliferation analysis of MYOD1-silenced CEF cells. CEF cells transfected with MYOD1 siRNAs were harvested at 48 hpt for CCK-8 assay. (D,E) RT-qPCR and western blot analysis of gga-miR-223 and IGF2 expressions in MYOD1-silenced CEF cells at 48 hpt. (F,G) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in CEFs overexpressing miR-M2-5p determined by RT-qPCR or western blot analysis. (H) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in CEFs overexpressing miR-M2-5p. (I,J) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in GaHV-2-infected CEFs determined by RT-qPCR or western blot analysis. (K) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in GaHV-2-infected CEFs. Numbers were normalized to the corresponding signal from the beta -actin bands. Error bars are derived from three independent replicates. Values of p indicated on columns were used for statistical analyses; ns, no significance. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33224130), licensed under a CC-BY license. Not internally tested by R&D Systems.

Proliferation and apoptosis of miR-M2-5p inhibitor-transfected MSB-1 cells. (A) RT-qPCR analysis of the relative expression levels of viral protein-coding or miRNA genes in MSB-1 cells at 24 hpt with miR-M2-5p inhibitor. (B,C) Cell viability and apoptosis of the MSB-1 cells transfected with miR-M2-5p inhibitor. MSB-1 cells transfected with miR-M2-5p inhibitor were harvested at 24 hpt for Annexin V-FITC and propidium iodide (PI) staining for apoptosis analysis using flow cytometry. CCK-8 assay was used to assess cell viability. (D) Western blot analysis of proteins associated with the cell proliferation or apoptosis in MSB-1 cells at 24 hpt. Numbers below the blots indicate relative band intensity normalized to beta -actin. Error bars are derived from three independent replicates. Values of p indicated on columns were used for statistical analyses; ns, no significance. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33224130), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2921U-100UG
Alexa Fluor 405	FAB2921V-100UG
Alexa Fluor 488	FAB2921G-100UG
Alexa Fluor 532	FAB2921X-100UG
Alexa Fluor 594	FAB2921T-100UG
Alexa Fluor 647	FAB2921R-100UG
Alexa Fluor 700	FAB2921N-100UG
Alexa Fluor 750	FAB2921S-100UG
Alexa Fluor Plus 405	FAB2921AFP405-100UG
Alexa Fluor Plus 488	FAB2921AFP488-100UG
Alexa Fluor Plus 555	FAB2921AFP555-100UG
Alexa Fluor Plus 594	FAB2921AFP594-100UG
Alexa Fluor Plus 647	FAB2921AFP647-100UG
Alexa Fluor Plus 680	FAB2921AFP680-100UG
Allophycocyanin	FAB2921A
Biotin	FAB2921B
CoraFluor 1	FAB2921CL1
DyLight 350	FAB2921D
DyLight 405	FAB2921E
DyLight 488	FAB2921K
DyLight 550	FAB2921L
DyLight 594	FAB2921M
DyLight 650	FAB2921W
DyLight 680	FAB2921Y
DyLight 755	FAB2921Z
FITC	FAB2921F
HRP	FAB2921H
Janelia Fluor 525	FAB2921JF525
Janelia Fluor 549	FAB2921I
Janelia Fluor 646	FAB2921J
Janelia Fluor 669	FAB2921JF669
PE	FAB2921P
PerCP	FAB2921C
mFluor Violet 450 SE	FAB2921MFV450
mFluor Violet 500 SE	FAB2921MFV500
mFluor Violet 610 SE	FAB2921MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2921-SP
Carrier Free	MAB2921-500

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NBP3-25520

IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

100 ul

NBP3-25520

Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Recombinant IGF2 protein were separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:2000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Mouse plasma (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ELISA: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, mature recombinant human IGF2 protein (533.33-8.33 nM). Coated protein was probed with IGF2 antibody [HL1982] (NBP3-25520) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.

Western Blot: IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free [NBP3-25520] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (NBP3-25520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

IGF2 antibody [HL2937] (5 μg/mL)Detection: HRP-conjugated IGF2 antibody [HL1982] (1 μg/mL). Please notice that the detection antibody requires HRP to function as the detection antibody when paired with capture antibody.Please contact us for the conjugation service.

Reactivity:

Hu, Mu, Po, Ca

Species Glossary

Reactivity: Hu, Mu, Po, Ca

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-48510

IGF-II/IGF2 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP2-48510

Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining of human duodenum shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: IGF-II/IGF2 Antibody [NBP2-48510] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-48510-25ul

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NBL1-11869

IGF-II/IGF2 Overexpression Lysate

0.1 mg

NBL1-11869

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

IGF2 Products By Gene Name

Recombinant Human IGF-II/IGF2 Protein, CF

Recombinant Mouse IGF-II/IGF2 Protein, CF

IGF-II/IGF2 Antibody [Unconjugated]

IGF-II/IGF2 Antibody [Unconjugated]

IGF-II/IGF2 Antibody (8H1) - BSA Free

Mouse IGF-II/IGF2 DuoSet ELISA, 15 Plate

IGF-II/IGF2 Antibody (75015) [Unconjugated]

IGF-II/IGF2 Antibody (122404) [Unconjugated]

IGF-II/IGF2 Antibody (75014) [Unconjugated]

IGF-II/IGF2 Antibody (HL1982) - Azide and BSA Free

IGF-II/IGF2 Antibody - BSA Free

IGF-II/IGF2 Overexpression Lysate