| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41264-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27400SS | |||
| Azide and BSA Free | NBP2-80774 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27169SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15486-20ul | |||
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27117-img0008.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis of decalcified, FFPE tissue sections from the paws of mice with collagen-induced arthritis using the HMGB1 antibody. The antibody was used at 5 ug/ml. Data courtesy of Dr Ulf Andersson, Karolinska Institute.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27117-img0007.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody [NBP2-27117] - Intracellular analysis using HMGB1 antibody. Human monocytes were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-27117 and APC secondary this antibody were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Flow-Cytometry-NBP2-27117-img0006.jpg)
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27117] - Lane view shows a specific band for HMGB1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27117-img0005.jpg)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-271120241932699.gif "<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Mouse Tissues.Loading: 15 ul of lysate Antibodies: HMGB1/HMG-1 , 1 ug/mL , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420405564.gif)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Rat Tissues.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-271120242074672.gif)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420392765.gif)
![Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in HL60 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling HMGB1/HMG-1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202419432287.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Human Stomach Tissue.Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-HMGB1/HMG-1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420312979.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HMGB1/HMG-1 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420392762.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-HMGB1/HMG-1 antibody at 1 ul/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420333866.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Analysis using HMGB1 antibody. A) Full-length human HMGB1 protein, B) human HeLa cell lysate and C) mouse NIH 3T3 cell lysate were probed with HMGB1 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N12A1-Western-Blot-NBP2-27400-img0004.jpg "Western Blot: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Analysis using HMGB1 antibody. A) Full-length human HMGB1 protein, B) human HeLa cell lysate and C) mouse NIH 3T3 cell lysate were probed with HMGB1 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: HMGB1/HMG-1 Antibody (19N12A1) [NBP2-27400] - Image shows a specific band for HMGB1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-19N12A1-Simple-Western-NBP2-27400-img0003.jpg)
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62782_mouse-hmgb1-hmg-1-elisa-kit-chemiluminescence-13220241624343.jpg "ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62782_mouse-hmgb1-hmg-1-elisa-kit-chemiluminescence-4320249151663.jpg)
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169] - Analysis using HMGB1 antibody. Mouse NIH 3T3 cell lysate was probed with HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27169-img0003.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27169] - Analysis using HMGB1 antibody. Mouse NIH 3T3 cell lysate was probed with HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27169] - Simple Western lane view shows a specific band for HMGB1 in 0.2 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27169-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0003.jpg "Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of C6 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0004.jpg)
![ELISA: Rat HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP3-06661] - Samples were spiked with high concentrations of Rat HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06661_rat-hmgb1-hmg-1-elisa-kit-colorimetric-13220241626124.jpg "ELISA: Rat HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP3-06661] - Samples were spiked with high concentrations of Rat HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP3-06661] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06661_rat-hmgb1-hmg-1-elisa-kit-colorimetric-4320249163312.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235] - Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate. All lanes: HMGB1/HMG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 25 KDa. Observed band size: 25 KDa.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1A1-Western-Blot-NBP3-15235-img0002.jpg "Western Blot: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235] - Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate. All lanes: HMGB1/HMG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 25 KDa. Observed band size: 25 KDa.")
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1A1) [NBP3-15235] - IHC image of HMGB1/HMG-1 antibody diluted at 1:100 and staining in paraffin-embedded human tonsil tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1A1-Immunohistochemistry-NBP3-15235-img0001.jpg)
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62781] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62781_human-hmgb1-hmg-1-elisa-kit-chemiluminescence-132202416225284.jpg "ELISA: Human HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62781] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62781] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62781_human-hmgb1-hmg-1-elisa-kit-chemiluminescence-43202495079.jpg)
![ELISA: Human HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62781] - Standard Curve from ELISA tested on Human cell culture supernatants. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-62781_human-hmgb1-hmg-1-elisa-kit-chemiluminescence-elisa-263202414748..jpg)
