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![N/A TIM-1/KIM-1/HAVCR [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_TIM1_DY1817_ELISA_1722.jpg "N/A TIM-1/KIM-1/HAVCR [Biotin]")
![ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-ELISA-NBP2-81119-img0001.jpg "ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.")
![Surface Plasmon Resonance: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - Surface plasmon resonance (SPR) sensorgram TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119]. 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein to immbolised the rabbit IgG chimeric version of 3B3 [NBP2-81119]. TIM-1 Fc-fusion protein was injected and allowed to associate with NBP2-81119 between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. TIM-1 Fc-fusion protein showed rapid binding to NBP2-81119 and following ligand removal remained strongly bound. This suggests that NBP2-81119 has a very high affinity for TIM-1 Fc-fusion protein.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-Surface-Plasmon-Resonance-NBP2-81119-img0002.jpg)
![Western Blot: TIM-1/KIM-1/HAVCR Antibody (JE39-41) [NBP3-33017] - Western blot analysis of TIM-1/KIM-1/HAVCR on different lysates with Rabbit anti-TIM-1/KIM-1/HAVCR antibody (NBP3-33017) at 1/1,000 dilution. Lane 1: Huh7 cell lysate Lane 2: HepG2 cell lysate Lane 3: NCI-H441 cell lysate Lane 4: Jurkat cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33017) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33017_rabbit-tim-1-kim-1-havcr-mab-je39-41-288202412493493.jpg "Western Blot: TIM-1/KIM-1/HAVCR Antibody (JE39-41) [NBP3-33017] - Western blot analysis of TIM-1/KIM-1/HAVCR on different lysates with Rabbit anti-TIM-1/KIM-1/HAVCR antibody (NBP3-33017) at 1/1,000 dilution. Lane 1: Huh7 cell lysate Lane 2: HepG2 cell lysate Lane 3: NCI-H441 cell lysate Lane 4: Jurkat cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33017) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![SDS-Page: Recombinant Human TIM-1/KIM-1/HAVCR Protein [NBP2-52037] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Recombinant-Protein-SDS-Page-NBP2-52037-img0001.jpg "SDS-Page: Recombinant Human TIM-1/KIM-1/HAVCR Protein [NBP2-52037] - 15% SDS Page (3 ug)")
![Sandwich ELISA: TIM-1/KIM-1/HAVCR Antibody Pair [HRP] [NBP2-79564] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79564-img0001.jpg "Sandwich ELISA: TIM-1/KIM-1/HAVCR Antibody Pair [HRP] [NBP2-79564] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")