| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2557-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87325-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44043 | |||
| BSA Free | NBP2-61424-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22170-50ul | |||
![Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-278202416285246.jpg "Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-298202414262694.jpg)
![Immunocytochemistry/ Immunofluorescence: Granulin Antibody (JE37-73) [NBP3-32371] - Immunocytochemistry analysis of THP-1 cells labeling Granulin with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-2920241114832.jpg)
![Flow Cytometry: Granulin Antibody (JE37-73) [NBP3-32371] - Flow cytometric analysis of THP-1 cells labeling Granulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32371, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-29202416453258.jpg)
![Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Western-Blot-NBP2-45915-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0003.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0004.jpg)
![Western Blot: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Western-Blot-NBP2-70847-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0004.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0002.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0003.jpg)
![Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70847-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87325] - Staining of human cell line A-431 shows localization to endoplasmic reticulum, endosomes & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87325-img0004.jpg "Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87325] - Staining of human cell line A-431 shows localization to endoplasmic reticulum, endosomes & lysosomes. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87325] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87325-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0004.jpg "Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).")
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human prostate shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0001.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human kidney shows cytoplasmic immunoreactivity in both renal glomeruli and tubules.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0003.jpg)
![Western Blot: Granulin Antibody (SR1971) [NBP3-22170] - Western blot analysis using NBP3-22170 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-22170_rabbit-granulin-mab-sr1971-572023152465.jpg "Western Blot: Granulin Antibody (SR1971) [NBP3-22170] - Western blot analysis using NBP3-22170 in 293T cell lysate.")
![Western Blot: Granulin Antibody (1F5) [H00002896-M01] - GRN monoclonal antibody (M01), clone 1F5 Analysis of GRN expression in HeLa.](http://images.novusbio.com/fullsize/Granulin-Antibody-1F5-Western-Blot-H00002896-M01-img0007.jpg "Western Blot: Granulin Antibody (1F5) [H00002896-M01] - GRN monoclonal antibody (M01), clone 1F5 Analysis of GRN expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: Granulin Antibody (1F5) [H00002896-M01] - Analysis of monoclonal antibody to GRN on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Granulin-Antibody-1F5-Immunocytochemistry-Immunofluorescence-H00002896-M01-img0005.jpg)
![Sandwich ELISA: Granulin Antibody (1F5) [H00002896-M01] - Detection limit for recombinant GST tagged GRN is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-1F5-Sandwich-ELISA-H00002896-M01-img0006.jpg)
![Western Blot: Granulin Antibody (4X3I7) [NBP3-16566] - Western blot analysis of extracts of A-431 cells, using Granulin Rabbit mAb (NBP3-16566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Granulin-Antibody-4X3I7-Western-Blot-NBP3-16566-img0001.jpg "Western Blot: Granulin Antibody (4X3I7) [NBP3-16566] - Western blot analysis of extracts of A-431 cells, using Granulin Rabbit mAb (NBP3-16566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Granulin Antibody (8O5Q5) [NBP3-16566] - Western blot analysis of various lysates using Granulin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16566_rabbit-granulin-mab-8o5q5-52202511361271.jpg)
![Western Blot: Granulin Antibody (8O5Q5) [Granulin] - Western blot analysis of various lysates using Granulin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16566_rabbit-granulin-mab-8o5q5-5220251718911.jpg)
![Western Blot: Granulin Antibody (4R8W1) [NBP3-33412] - Western blot analysis of lysates from Mouse liver, using Granulin Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33412_rabbit-granulin-mab-4r8w1-912202415175269.jpg "Western Blot: Granulin Antibody (4R8W1) [NBP3-33412] - Western blot analysis of lysates from Mouse liver, using Granulin Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: Granulin Antibody (4R8W1) [NBP3-33412] - Western blot analysis of lysates from HeLa cells, using Granulin Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33412_rabbit-granulin-mab-4r8w1-91220241514362.jpg)
![Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-26396] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing ER/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-26396-img0001.jpg "Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-26396] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing ER/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![ELISA: Rat Progranulin/PGRN ELISA Kit (Colorimetric) [NBP2-82200] - Samples were spiked with high concentrations of Rat Progranulin/PGRN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82200_rat-progranulin-pgrn-elisa-kit-colorimetric-132202416251746.jpg "ELISA: Rat Progranulin/PGRN ELISA Kit (Colorimetric) [NBP2-82200] - Samples were spiked with high concentrations of Rat Progranulin/PGRN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Progranulin/PGRN ELISA Kit (Colorimetric) [NBP2-82200] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82200_rat-progranulin-pgrn-elisa-kit-colorimetric-432024973671.jpg)