CTGF/CCN2: Novus Biologicals

NB100-724

CTGF/CCN2 Antibody - BSA Free

(13 Publications)

0.1 ml

NB100-724

Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Analysis of astrocyte reactivity, the cytoskeleton & migration rate following treatment with TGF-beta 2 & CCN2/CTGF. (A) GFAP immunoreactivity (green) is increased in murine ON astrocytes following TGF-beta 2 & CCN2/CTGF treatment. Nuclei are stained with Dapi (blue) (B) Western Blot experiments analyses of alpha -actinin protein synthesis in murine ON astrocytes after the treatment with 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF & 100 ng/ml CCN2/CTGF for 24 h. Densitometric analyses of Western Blot analyses shows the significant increase in alpha -actinin protein synthesis. Mean value of untreated cells (control) was set at 1. GAPDH was used to normalize protein intensity (n = 4; 50 ng CCN2/CTGF: *p = 0.029; 100 ng CCN2/CTGF: *p = 0.015; unpaired two-tailed t-test). (C) Scratch assay analyses show a significant increased migration rate for murine ON astrocytes after the treatment with ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF & 100 ng/ml CCN2/CTGF for 12 h. Mean value of migration rate of control cells was set to 100% (control: n = 15; TGF-beta 2: n = 12, **p = 0.008; 50 ng/ml CCN2/CTGF: n = 10, *p = 0.001; 100 ng/ml CCN2/CTGF: n = 4, **p = 0.0001). Data represented as mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CTGF/CCN2 Antibody [NB100-724] - GFAP expression & synthesis in the ON & ONH in a murine glaucoma model. (A) GFAP immunoreactivity in the glial lamina of 1-month (green, upper panel) & 2 months (green, middle & lower panel) old beta B1-CTGF1 mice compared to WT controls. Immunoreactivity of GFAP not altered in 1-month old TG & WT animals (green, upper panel). 2 month old beta B1-CTGF1 mice showed an increased GFAP immunoreactivity compared to WT control (green, middle panel). Lower panel shows a magnified detail of the middle panel. Nuclei are stained w/ Dapi (blue). (B) Schmatic illustration of the optic nerve. A depicts region in the glial lamina where cross sections obtained. ONH (unmyelinated part) & ON (myelinated part) used for molecular analysis. (C) Quantification of immunohistochemical staining of GFAP in the glial lamina is not altered in 1 month old TG & WT animals (WT: n = 5; TG: n = 4). GFAP immunoreactivity is increased in 2 month old TG animals compared to WT littermates (WT: n = 10, TG: n = 13; *p = 0.039; two-tailed t-test compared to oretical mean of 1 (normalized control)). Mean value of WT animals (control) set at 1. (D) RT-PCR analyses revealed no alteration in the Gfap mRNA expression in the ON of 1-month (WT: n = 7; TG: n = 5) & 2 month old beta B1-CTGF1 mice (WT: n = 15; TG: n = 16) compared to WT controls. In the ONH the Gfap mRNA expression is increased in the 2 month old TG compared to WT animals (WT: n = 6, TG: n = 5; *p = 0.04). The mRNA expression of Gfap is not altered in 1 month old animals (WT: n = 6, TG: n = 5). Mean value of WT animals (control) set at 1. RPL32 used to normalize mRNA expression. Data represented as mean ± SEM. PRL, Prelaminar region; PSL, Postlaminar region; ON, optic nerve; ONH, optic nerve head. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Analyses of ECM components in murine ON astrocytes following treatment w/ TGF-beta 2 & CCN2/CTGF. (A) Immunocytochemical staining against fibronectin (green, upper panel) showed a markedly increase following the treatment w/ 1 ng/ml TGF-beta 2 or 50 ng/ml CCN2/CTGF. Immunocytochemical staining against tropoelastin (green, lower panel) showed a pronounced increase after treatment w/ 1 ng/ml TGF-beta 2 or 50 ng/ml CCN2/CTGF. Nuclei stained w/ Dapi (blue). (B) Real-time RT-PCR analyses shows an intense upregulation of collagen 3 alpha 1, tropoelastin & fibronectin mRNA in murine ON astrocytes after treatment w/ 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF or 100 ng/ml CCN2/CTGF for 24 h compared to untreated control cells (collagen 3 alpha 1: n = 3; TGF-beta 2 ***p = 0.0000004, 50 ngCTGF **p = 0.005, 100ngCTGF **p = 0.008; fibronectin: n = 6, TGF-beta 2 *p = 0.00004, 50 ng CTGF *p = 0.016, 100 ngCTGF *p = 0.05; elastin: n = 5, TGF-beta 2 *p = 0.026, 50 ng CTGF *0.04, 100 ng CTGF *p = 0.03). mRNA expression normalized to Gnb2l & mean value of control cells set at 1. (C) Western Blot analysis show an increase in protein synthesis of collagen 3 alpha 1, elastin & fibronectin in murine ON astrocytes after treatment w/ 1 ng/ml TGF-beta 2, 50 ng/ml CCN2/CTGF or 100 ng/ml CCN2/CTGF for 24 h compared to untreated control cells (collagen 3 alpha 1: n = 3, TGF-beta 2 *p = 0.03, 100 ngCTGF **p = 0.009; fibronectin: n = 5, **p = 0.005, 50 ngCTGF **p = 0.006, 100 ng CTGF *p = 0.02; tropoelastin: n = 5, TGF-beta 2 ***p = 0.0004, 50 ngCTGF **p = 0.002, 100 ngCTGF ***p = 0.00001). GAPDH & alpha -tubulin used to normalize protein synthesis & mean value of control cells set at 1. Right panel shows representative Western Blots for all three proteins. Data represented as mean ± SD. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - Increasing substratum stiffness causes alterations in murine ON astrocyte reactivity, actin cytoskeleton & CCN2/CTGF level. (A) Filamentous actin labeled by phalloidin (red, upper panel) is increased when murine ON astrocytes were cultured on substrates with higher stiffness (30 kPa, 60 kPa) compared to softer control (10 kPa). Increasing substratum stiffness leads to an enhanced formation of actin stress fibers. Immunoreactivity of CCN2/CTGF (green, middle panel) is markedly increased when murine ON astrocytes were grown on substrates with higher stiffness (30, 60 kPa) compared to softer control (10 kPa). Murine ON astrocytes show an enhanced GFAP protein synthesis (green, lower panel) when cultured on 30 or 60 kPa compared to 10 kPa. Nuclei were stained with Dapi (blue). (B) CCN2/CTGF protein synthesis is significantly increased in murine ON astrocytes grown on 60 kPa compared to 10 kPa (10 kPa: n = 5; 30 kPa: n = 5; 60 kPa: n = 5; *p = 0.04; unpaired two-tailed t-test). (C) Protein synthesis of GFAP (10 kPa: n = 6; 30 kPa: n = 6, **p = 0.005; 60 kPa: n = 4, *p = 0.04; unpaired two-tailed t-test) show an enhanced reactivity of murine ON astrocytes cultured on 30 kPa or 60 kPa compared to 10 kPa control. (D) Vimentin protein synthesis is significantly increased in murine ON astrocytes cultured on 60 kPa compared to 10 kPa (10 kPa: n = 4; 30 kPa: n = 4; 60 kPa: n = 3 ***p = 0.00002). Protein synthesis was normalized to alpha -tubulin & mean value of control (10 kPa) was set at 1. Data represented as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35493079), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

CTGF expression correlates with invasiveness of mesenchymal transformed and TNBC cells. (A) Assessment of CTGF mRNA expression in different breast cancer cell lines using quantitative real-time PCR. Data represent mean +/- SEM. MCF-7-EMT n = 6, MDA-MB-231 n = 4 using unpaired, two-tailed t-test analysis to respective control (MCF-7). *P < 0.05; **P < 0.01 (B) Quantification and representative experiments of CTGF protein expression in different breast cancer cell lines compared to non-invasive MCF-7 breast cancer cell line. CTGF band intensity was quantified by densitometry and normalized to GAPDH. Lower panel shows loading control GAPDH that was detected in the same sample and were run in the same gel lane and detected in the same Western blot membrane. Data represent mean +/- SEM. n = 6 using unpaired, two-tailed t-test analysis to respective control (MCF-7). *P < 0.05; **P < 0.01 (C) Patient tissue sections (n = 24) were analyzed for CTGF expression. Representative images of normal breast tissue (right panel) and IDC (invasive ductal carcinoma, left panel) are illustrated. (D) Graph illustrating distribution of CTGF expression within two different analyzed patient sample categories. (E) Results of three independent flow cytometry experiments of CD51 and CD106 co-expression in MCF-7 (circle), MCF-7-EMT (square) and MDA-MB-231 (triangle) breast cancer cell lines. Data represent mean +/- SEM. MCF-7-EMT, MDA-MB-231 n = 3 using unpaired, two-tailed t-test analysis to respective control (MCF-7). *P < 0.05 (F) Proportion of CD51 to CD106 was asses using flow cytometry after staining with fluorescence-labeled antibodies. A representative experiment to E is illustrated. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33087801), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Sh

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-724AF350
Alexa Fluor 405	NB100-724AF405
Alexa Fluor 488	NB100-724AF488
Alexa Fluor 532	NB100-724AF532
Alexa Fluor 594	NB100-724AF594
Alexa Fluor 647	NB100-724AF647
Alexa Fluor 700	NB100-724AF700
Alexa Fluor 750	NB100-724AF750
Alexa Fluor Plus 405	NB100-724AFP405
Alexa Fluor Plus 488	NB100-724AFP488
Alexa Fluor Plus 555	NB100-724AFP555
Alexa Fluor Plus 594	NB100-724AFP594
Alexa Fluor Plus 647	NB100-724AFP647
Alexa Fluor Plus 680	NB100-724AFP680
Allophycocyanin	NB100-724APC
Biotin	NB100-724B
CoraFluor 1	NB100-724CL1
DyLight 350	NB100-724UV
DyLight 405	NB100-724V
DyLight 488	NB100-724G
DyLight 550	NB100-724R
DyLight 594	NB100-724DL594
DyLight 650	NB100-724C
DyLight 680	NB100-724FR
DyLight 755	NB100-724IR
FITC	NB100-724F
HRP	NB100-724H
Janelia Fluor 525	NB100-724JF525
Janelia Fluor 549	NB100-724JF549
Janelia Fluor 646	NB100-724JF646
Janelia Fluor 669	NB100-724JF669
PE	NB100-724PE
PerCP	NB100-724PCP
mFluor Violet 450 SE	NB100-724MFV450
mFluor Violet 500 SE	NB100-724MFV500
mFluor Violet 610 SE	NB100-724MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-724SS

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MAB660

CTGF/CCN2 Antibody (88430) - C-terminus

(10 Publications)

100 μg

MAB660

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human CTGF/CCN2 C-Terminus Monoclonal Antibody (Catalog # MAB660) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Human CTGF/CCN2 C-Terminus Monoclonal Antibody (Catalog # MAB660) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Immunostaining of neuroendocrine tumors. Representative photomicrographs demonstrating anti-connective tissue growth factor (CCN2) (panel A) immunostaining of gut carcinoid tumor tissue. CCN2 immunoreactivity was seen in cells corresponding to chromogranin A-positive tumor cells and gut smooth muscle (denoted by arrows in panels B and C, respectively). All sections were counterstained with hematoxylin. Magnification ×50. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20053285), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB660U-100UG
Alexa Fluor 405	FAB660V-100UG
Alexa Fluor 488	FAB660G-100UG
Alexa Fluor 532	FAB660X-100UG
Alexa Fluor 594	FAB660T-100UG
Alexa Fluor 647	FAB660R-100UG
Alexa Fluor 700	FAB660N-100UG
Alexa Fluor 750	FAB660S-100UG
Alexa Fluor Plus 405	FAB660AFP405-100UG
Alexa Fluor Plus 488	FAB660AFP488-100UG
Alexa Fluor Plus 555	FAB660AFP555-100UG
Alexa Fluor Plus 594	FAB660AFP594-100UG
Alexa Fluor Plus 647	FAB660AFP647-100UG
Alexa Fluor Plus 680	FAB660AFP680-100UG
Allophycocyanin	FAB660A
Biotin	FAB660B
CoraFluor 1	FAB660CL1
DyLight 350	FAB660D
DyLight 405	FAB660E
DyLight 488	FAB660K
DyLight 550	FAB660L
DyLight 594	FAB660M
DyLight 650	FAB660W
DyLight 680	FAB660Y
DyLight 755	FAB660Z
FITC	FAB660F
HRP	FAB660H
Janelia Fluor 525	FAB660JF525
Janelia Fluor 549	FAB660I
Janelia Fluor 646	FAB660J
Janelia Fluor 669	FAB660JF669
PE	FAB660P
PerCP	FAB660C
mFluor Violet 450 SE	FAB660MFV450
mFluor Violet 500 SE	FAB660MFV500
mFluor Violet 610 SE	FAB660MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB660-SP
Carrier Free	MAB660-500

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AF660

CTGF/CCN2 Antibody [Unconjugated] - C-terminus

(7 Publications)

100 μg

AF660

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

<b>Genetic Strategies Validation. </b>Effect of KIAA1199 knock-down and over-expression on the expression of PLXNB3, SEMA5A and CTGF in fibroblast-like synoviocyte (FLS) cells. (A). Real-time PCR of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 24 h in FLS cells; beta -actin was used as a loading control. (B). Western blotting of KIAA1199, PLXNB3, SEMA5A and CTGF transfected with scrambled siRNA, KIAA1199 siRNA duplex, pcDNA3.2DEST and pcDNA3.2DEST-KIAA1199 for 48 h in FLS cells; beta -actin was used as a loading control. All experiments were performed at least in triplicates, the data are presented as mean ± SD; *P <0.05, compared with control (*significant differences). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26022278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF660U-100UG
Alexa Fluor 405	AF660V-100UG
Alexa Fluor 488	AF660G-100UG
Alexa Fluor 532	AF660X-100UG
Alexa Fluor 594	AF660T-100UG
Alexa Fluor 647	AF660R-100UG
Alexa Fluor 700	AF660N-100UG
Alexa Fluor 750	AF660S-100UG
Alexa Fluor Plus 405	AF660AFP405-100UG
Alexa Fluor Plus 488	AF660AFP488-100UG
Alexa Fluor Plus 555	AF660AFP555-100UG
Alexa Fluor Plus 594	AF660AFP594-100UG
Alexa Fluor Plus 647	AF660AFP647-100UG
Alexa Fluor Plus 680	AF660AFP680-100UG
Allophycocyanin	AF660A
Biotin	BAF660
CoraFluor 1	AF660CL1
DyLight 350	AF660D
DyLight 405	AF660E
DyLight 488	AF660K
DyLight 550	AF660L
DyLight 594	AF660M
DyLight 650	AF660W
DyLight 680	AF660Y
DyLight 755	AF660Z
FITC	AF660F
HRP	AF660H
Janelia Fluor 525	AF660JF525
Janelia Fluor 549	AF660I
Janelia Fluor 646	AF660J
Janelia Fluor 669	AF660JF669
PE	AF660P
PerCP	AF660C
mFluor Violet 450 SE	AF660MFV450
mFluor Violet 500 SE	AF660MFV500
mFluor Violet 610 SE	AF660MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF660-SP

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NBP2-16025

CTGF/CCN2 Antibody

(1 Review)

(4 Publications)

0.1 ml

NBP2-16025

HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (NBP2-16025) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Pm, Po, Ch, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Pm, Po, Ch, Pm, Sh

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-61415

CTGF/CCN2 Antibody (CL5339) - BSA Free

100 ul

NBP2-61415

Staining of mouse cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Staining of rat cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Staining of human cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43982AF350
Alexa Fluor 405	NBP3-43982AF405
Alexa Fluor 488	NBP3-43982AF488
Alexa Fluor 532	NBP3-43982AF532
Alexa Fluor 594	NBP3-43982AF594
Alexa Fluor 647	NBP3-43982AF647
Alexa Fluor 700	NBP3-43982AF700
Alexa Fluor 750	NBP3-43982AF750
Alexa Fluor Plus 405	NBP3-43982AFP405
Alexa Fluor Plus 488	NBP3-43982AFP488
Alexa Fluor Plus 555	NBP3-43982AFP555
Alexa Fluor Plus 594	NBP3-43982AFP594
Alexa Fluor Plus 647	NBP3-43982AFP647
Alexa Fluor Plus 680	NBP3-43982AFP680
Allophycocyanin	NBP3-43982APC
Biotin	NBP3-43982B
CoraFluor 1	NBP3-43982CL1
DyLight 350	NBP3-43982UV
DyLight 405	NBP3-43982V
DyLight 488	NBP3-43982G
DyLight 550	NBP3-43982R
DyLight 594	NBP3-43982DL594
DyLight 650	NBP3-43982C
DyLight 680	NBP3-43982FR
DyLight 755	NBP3-43982IR
FITC	NBP3-43982F
HRP	NBP3-43982H
Janelia Fluor 525	NBP3-43982JF525
Janelia Fluor 549	NBP3-43982JF549
Janelia Fluor 646	NBP3-43982JF646
Janelia Fluor 669	NBP3-43982JF669
PE	NBP3-43982PE
PerCP	NBP3-43982PCP
mFluor Violet 450 SE	NBP3-43982MFV450
mFluor Violet 500 SE	NBP3-43982MFV500
mFluor Violet 610 SE	NBP3-43982MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43982
BSA Free	NBP2-61415-25ul

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NBP2-75010

Mouse CTGF/CCN2 ELISA Kit (Colorimetric)

(1 Publication)

1 Kit

NBP2-75010

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

0.16 - 10 ng/mL

Sensitivity:

0.1 ng/mL

Sample Type:

Serum, Plasma, Urine,Tissue Homogenate

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NBP2-75009

Human CTGF/CCN2 ELISA Kit (Colorimetric)

(1 Publication)

1 Kit

NBP2-75009

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

62.50 - 4000 pg/mL

Sensitivity:

37.5 pg/mL

Sample Type:

Serum, Plasma, Saliva, Urine, Milk, Cell Supernatant

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NBP2-16026

CTGF/CCN2 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP2-16026

HepG2 conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (NBP2-16026) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, Sh

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-69990

CTGF/CCN2 Antibody

100 μg

NBP2-69990

Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NBP2-69990] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Control: CTGF/CCN2 Antibody [NBP2-69990] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP2-66426

CTGF/CCN2 Antibody

100 μg

NBP2-66426

Control: CTGF/CCN2 Antibody [NBP2-66426] - Negative Control showing staining of paraffin embedded Rat Liver, with no primary antibody.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP3-13686

CTGF/CCN2 Antibody (28711) - Azide and BSA Free

100 ul

NBP3-13686

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB100-724PEP

CTGF/CCN2 Antibody Blocking Peptide

0.05 mg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

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Species Glossary

CCN2 Products By Gene Name

CTGF/CCN2 Antibody - BSA Free

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Mouse CTGF/CCN2 ELISA Kit (Colorimetric)

Human CTGF/CCN2 ELISA Kit (Colorimetric)

CTGF/CCN2 Antibody - BSA Free

CTGF/CCN2 Antibody

CTGF/CCN2 Antibody

CTGF/CCN2 Antibody (28711) - Azide and BSA Free

CTGF/CCN2 Antibody Blocking Peptide