CCL3 Products By Gene Name

HAF018). A specific band was detected for CCL3/MIP-1a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CCL3/MIP-1a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL3/MIP-1a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

6625-MA) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252). The ND₅₀ is typically 0.075-0.45 µg/mL." class="big_lightbox"> 6625-MA) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252). The ND₅₀ is typically 0.075-0.45 µg/mL." class="big_thumb" />

MAB004, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB004, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="U266 cells were stained with Mouse Anti-Human CCL3/CCL4 Monoclonal Antibody (Catalog # MAB2701, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

HAF005). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB4501) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of stimulated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of stimulated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

1061-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Cotton Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB1061). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_lightbox"> 1061-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Cotton Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB1061). The ND₅₀ is typically 0.05-0.3 µg/mL." alt="Recombinant Cotton Rat CCL3/MIP-1a (Catalog # 1061-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Cotton Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB1061). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_thumb" />

270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant HumanCCL3/MIP-1a (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP-1a Polyclonal Antibody (Catalog # AB-270-NA). The ND₅₀ is typically ≤ 6 µg/mL." class="big_lightbox"> 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant HumanCCL3/MIP-1a (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP-1a Polyclonal Antibody (Catalog # AB-270-NA). The ND₅₀ is typically ≤ 6 µg/mL." alt="Recombinant Human CCL3/MIP-1a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant HumanCCL3/MIP-1a (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP-1a Polyclonal Antibody (Catalog # AB-270-NA). The ND₅₀ is typically ≤ 6 µg/mL." class="big_thumb" />

NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="CCL3/MIP-1a was detected in perfusion fixed frozen sections of rat kidney using Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66251) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB6625) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

1061-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1061). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> 1061-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1061). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Cotton Rat CCL3/MIP-1a (Catalog # 1061-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Cotton Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1061). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

HAF016). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6625) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF007). A specific band was detected for CCL3/MIP-1a at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." class="big_lightbox"> HAF007). A specific band was detected for CCL3/MIP-1a at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." alt="Western blot shows lysates of A-72 canine fibroma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Canine CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB7370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL3/MIP-1a at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." class="big_thumb" />

450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB450). The ND₅₀ is typically 0.125-0.75 µg/mL." class="big_lightbox"> 450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB450). The ND₅₀ is typically 0.125-0.75 µg/mL." alt="Recombinant MouseCCL3/MIP-1a (Catalog # 450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB450). The ND₅₀ is typically 0.125-0.75 µg/mL." class="big_thumb" />