| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83435-25ul | |||
HAF016). A specific band was detected for Biliverdin Reductase B/BLVRB at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Biliverdin Reductase B/BLVRB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Biliverdin Reductase B/BLVRB at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6568-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83434-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00081-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90188-50ul | |||
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human colon, liver, small intestine and spleen using Anti-BLVRB antibody NBP1-83435 (A) shows similar protein distribution across tissues to independent antibody NBP1-83434 (B).](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83435-img0013.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human colon, liver, small intestine and spleen using Anti-BLVRB antibody NBP1-83435 (A) shows similar protein distribution across tissues to independent antibody NBP1-83434 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83435-img0007.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83435-img0020.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83435-img0016.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83435-img0017.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83435-img0019.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83435] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83435-img0018.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83434] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83434-img0011.jpg "Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83434] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-83434] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Western-Blot-NBP1-83434-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83434] - Staining of human colon, liver, small intestine and spleen using Anti-BLVRB antibody NBP1-83434 (A) shows similar protein distribution across tissues to independent antibody NBP1-83435 (B).](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83434-img0007.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83434] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83434-img0009.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83434] - Staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83434-img0010.jpg)
![Immunohistochemistry-Paraffin: Biliverdin Reductase B/BLVRB Antibody [NBP1-83434] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunohistochemistry-Paraffin-NBP1-83434-img0012.jpg)
![Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP3-00081] - Anti-Biliverdin Reductase B/BLVRB rabbit polyclonal antibody at 1:500 dilution. Lane A: Biliverdin Reductase B/BLVRB knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Western-Blot-NBP3-00081-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP3-00081] - Anti-Biliverdin Reductase B/BLVRB rabbit polyclonal antibody at 1:500 dilution. Lane A: Biliverdin Reductase B/BLVRB knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa")
![<b>Genetic Strategies Validation. </b>Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP3-00081] - Anti-Biliverdin Reductase B/BLVRB rabbit polyclonal antibody at 1:500 dilution. Lane A: Caco-2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Western-Blot-NBP3-00081-img0006.jpg)
![Immunoprecipitation: Biliverdin Reductase B/BLVRB Antibody [NBP3-00081] - Biliverdin Reductase B/BLVRB was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. Lane D:0.5 mg Caco-2 Whole Cell Lysate. 2 ul anti-Biliverdin Reductase B/BLVRB rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Biliverdin Reductase B/BLVRB rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Immunoprecipitation-NBP3-00081-img0004.jpg)
![Western Blot: Biliverdin Reductase B/BLVRB Antibody (2F4) [H00000645-M09] - Analysis of BLVRB expression in transfected 293T cell line by BLVRB monoclonal antibody (M09), clone 2F4. Lane 1: BLVRB transfected lysatE (22.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-2F4-Western-Blot-H00000645-M09-img0002.jpg "Western Blot: Biliverdin Reductase B/BLVRB Antibody (2F4) [H00000645-M09] - Analysis of BLVRB expression in transfected 293T cell line by BLVRB monoclonal antibody (M09), clone 2F4. Lane 1: BLVRB transfected lysatE (22.1 KDa). Lane 2: Non-transfected lysate.")
![ELISA: Biliverdin Reductase B/BLVRB Antibody (2F4) [H00000645-M09] - Detection limit for recombinant GST tagged BLVRB is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-2F4-ELISA-H00000645-M09-img0001.jpg)
![Western Blot: Biliverdin Reductase B/BLVRB Antibody (009) [NBP2-90188] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-009-Western-Blot-NBP2-90188-img0001.jpg "Western Blot: Biliverdin Reductase B/BLVRB Antibody (009) [NBP2-90188] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa")
![Immunoprecipitation: Biliverdin Reductase B/BLVRB Antibody (009) [NBP2-90188] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg K562 Whole Cell LysateLane D:0.5 mg Caco-2 Whole Cell Lysate2 uL anti-BLVRB rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BLVRB rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-009-Immunoprecipitation-NBP2-90188-img0002.jpg)
![ELISA: Human Biliverdin Reductase B/BLVRB ELISA Kit (Colorimetric) [NBP2-69974] - Samples were spiked with high concentrations of Human Biliverdin Reductase B/BLVRB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69974_human-biliverdin-reductase-b-blvrb-elisa-kit-colorimetric-132202416221071.jpg "ELISA: Human Biliverdin Reductase B/BLVRB ELISA Kit (Colorimetric) [NBP2-69974] - Samples were spiked with high concentrations of Human Biliverdin Reductase B/BLVRB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Biliverdin Reductase B/BLVRB ELISA Kit (Colorimetric) [NBP2-69974] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69974_human-biliverdin-reductase-b-blvrb-elisa-kit-colorimetric-4320249115678.jpg)
![Immunoprecipitation:Biliverdin Reductase B/BLVRB AntibodyNBP3-29053] -Detection of human BLVRB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLVRB antibody NBP3-29053 (lot NBP3-29053-1) used for IP at 6 µg per reaction. BLVRB was also immunoprecipitated by rabbit anti-BLVRB Antibody.. For blotting immunoprecipitated BLVRB, NBP3-29053 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29053_rabbit-biliverdin-reductase-b-blvrb-pab-301220241321390.jpg "Immunoprecipitation:Biliverdin Reductase B/BLVRB AntibodyNBP3-29053] -Detection of human BLVRB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLVRB antibody NBP3-29053 (lot NBP3-29053-1) used for IP at 6 µg per reaction. BLVRB was also immunoprecipitated by rabbit anti-BLVRB Antibody.. For blotting immunoprecipitated BLVRB, NBP3-29053 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:Biliverdin Reductase B/BLVRB AntibodyNBP3-30692] -Detection of human BLVRB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLVRB antibody NBP3-30692 (lot NBP3-30692-1) used for IP at 6 µg per reaction. BLVRB was also immunoprecipitated by rabbit anti-BLVRB.. For blotting immunoprecipitated BLVRB, NBP3-30692 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30692_rabbit-biliverdin-reductase-b-blvrb-pab-301220241229390.jpg "Immunoprecipitation:Biliverdin Reductase B/BLVRB AntibodyNBP3-30692] -Detection of human BLVRB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLVRB antibody NBP3-30692 (lot NBP3-30692-1) used for IP at 6 µg per reaction. BLVRB was also immunoprecipitated by rabbit anti-BLVRB.. For blotting immunoprecipitated BLVRB, NBP3-30692 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-31143] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE Flavin reductase antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Western-Blot-NBP1-31143-img0003.jpg "Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-31143] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE Flavin reductase antibody, antibody diluted at 1:1000.")
![Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-53068] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Biliverdin-Reductase-B-BLVRB-Antibody-Western-Blot-NBP1-53068-img0002.jpg "Western Blot: Biliverdin Reductase B/BLVRB Antibody [NBP1-53068] - Human Liver cell lysate, concentration 0.2-1 ug/ml.")
