| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43945 | |||
| BSA Free | NBP2-52908-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43944 | |||
| BSA Free | NBP2-52907-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38630-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33857-25ul | |||
![Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Lane 1: Marker [kDa] Lane 2:Human cell line U-251 MG](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Western-Blot-NBP2-52908-img0016.jpg "Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Lane 1: Marker [kDa] Lane 2:Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in U251 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunocytochemistry-Immunofluorescence-NBP2-52908-img0017.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunohistochemistry-Paraffin-NBP2-52908-img0025.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANLN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Western-Blot-NBP2-52908-img0015.jpg)
![Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Interaction of Anillin with GST-fused protein: MDA-MB-231 cells were lysed in TBS, pH 7.5, containing 0.75% Triton X-100 with inhibitors of proteases and phosphatases. Lysate was sonicated and cleared by centrifugation. for the input, sample buffer was added to part of the lysate and heated fro 10min 95 C. The rest of the lysate was incubated with either GST or GST-fused protein immobilized on agarose beads for 2h in 4 C. the beads were washed three times in lysis buffer, resuspended in sample buffer and heated for 10min 95 C. The samples were resolved using SDS-PAGE and immunoblotted with anti-ANLN antibody. This image was submitted by customer review.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Western-Blot-NBP2-52908-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in A431 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunocytochemistry-Immunofluorescence-NBP2-52908-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in HeLa cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunocytochemistry-Immunofluorescence-NBP2-52908-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in MCF7 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunocytochemistry-Immunofluorescence-NBP2-52908-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in U2OS cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunocytochemistry-Immunofluorescence-NBP2-52908-img0005.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human colorectal cancer shows moderate to strong nuclear positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunohistochemistry-Paraffin-NBP2-52908-img0020.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunohistochemistry-Paraffin-NBP2-52908-img0021.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human rectum shows moderate to strong nuclear positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunohistochemistry-Paraffin-NBP2-52908-img0022.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human skin shows moderate to strong nuclear positivity in a subset of dermal cells.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunohistochemistry-Paraffin-NBP2-52908-img0023.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0303-Immunohistochemistry-Paraffin-NBP2-52908-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of U-251 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0301-Immunocytochemistry-Immunofluorescence-NBP2-52907-img0013.jpg "Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of U-251 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0301-Immunohistochemistry-Paraffin-NBP2-52907-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0301) [NBP2-52907] - Staining of A-431 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0301-Immunocytochemistry-Immunofluorescence-NBP2-52907-img0014.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human lung cancer shows moderate to strong nuclear positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0301-Immunohistochemistry-Paraffin-NBP2-52907-img0007.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0301-Immunohistochemistry-Paraffin-NBP2-52907-img0008.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human skin shows moderate to strong nuclear positivity in a subset of dermal cells.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0301-Immunohistochemistry-Paraffin-NBP2-52907-img0009.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human rectum shows moderate to strong nuclear positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0301-Immunohistochemistry-Paraffin-NBP2-52907-img0010.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody (CL0301) [NBP2-52907] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/Anillin-Antibody-CL0301-Immunohistochemistry-Paraffin-NBP2-52907-img0011.jpg)
![Western Blot: Anillin Antibody [NBP2-38630] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ANLN antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Anillin-Antibody-Western-Blot-NBP2-38630-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Anillin Antibody [NBP2-38630] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ANLN antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Anillin Antibody [NBP2-38630] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38630-img0014.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-38630-img0019.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-38630-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Analysis in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-38630-img0015.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human colon shows moderate to strong nuclear positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-38630-img0016.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human placenta shows moderate to strong nuclear positivity in a subset of trophoblastic cells.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-38630-img0017.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-38630] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-38630-img0018.jpg)
![Western Blot: Anillin Antibody [NBP2-33857] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ANLN antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Anillin-Antibody-Western-Blot-NBP2-33857-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Anillin Antibody [NBP2-33857] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ANLN antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-33857] - Staining in human testis and skeletal muscle tissues using anti-ANLN antibody. Corresponding ANLN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-33857-img0012.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-33857] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-33857-img0010.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody [NBP2-33857] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NBP2-33857-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1314-img0003.jpg "Immunocytochemistry/Immunofluorescence: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: Anillin Antibody [NB100-1314] - 10ug/ml staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NB100-1314-img0002.jpg)
![Flow Cytometry: Anillin Antibody [NB100-1314] - Analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Anillin-Antibody-Flow-Cytometry-NB100-1314-img0004.jpg)
![Western Blot: Anillin Antibody [NB100-68216] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-68216 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Anillin was also immunoprecipitated by NB100-68215, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Anillin-Antibody-Western-Blot-NB100-68216-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Anillin Antibody [NB100-68216] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-68216 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Anillin was also immunoprecipitated by NB100-68215, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: Anillin Antibody [NB100-68216] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Anillin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-NB100-68216-img0004.jpg)
![Immunohistochemistry-Paraffin: Anillin Antibody [NB100-68216] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-Anillin used at a dilution of 1:250.](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunohistochemistry-Paraffin-NB100-68216-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Anillin Antibody [NB100-41099] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41099-img0002.jpg "Immunocytochemistry/Immunofluorescence: Anillin Antibody [NB100-41099] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunocytochemistry/Immunofluorescence: Anillin Antibody [NB100-41099] - Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Anillin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41099-img0001.jpg)
![Western Blot: Anillin Overexpression Lysate (Adult Normal) [NBP2-06787] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Anillin.](http://images.novusbio.com/fullsize/Anillin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06787-img0001.jpg "Western Blot: Anillin Overexpression Lysate (Adult Normal) [NBP2-06787] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Anillin.")
![ELISA: Human Anillin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31086] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31086_human-anillin-ready-to-use-elisa-kit-colorimetric-206202415301527.png "ELISA: Human Anillin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31086] - Standard Curve Reference")
![ELISA: Human Anillin ELISA Kit (Colorimetric) [NBP3-27212] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27212_human-anillin-elisa-kit-colorimetric-114202410251017.png "ELISA: Human Anillin ELISA Kit (Colorimetric) [NBP3-27212] - Standard Curve Reference")