| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7186-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70173 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89600-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-20029-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26509-50ul | |||
![Western Blot: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Western blot analysis of Angiopoietin-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: human liver tissue lysate Lane 3: human placenta tissue lysate Lane 4: mouse liver tissue lysate](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-JM71-34-Western-Blot-NBP2-67154-img0006.jpg "Western Blot: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Western blot analysis of Angiopoietin-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: human liver tissue lysate Lane 3: human placenta tissue lysate Lane 4: mouse liver tissue lysate")
![Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Analysis of paraffin-embedded human placenta tissue using anti-Angiopoietin 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-JM71-34-Immunohistochemistry-Paraffin-NBP2-67154-img0005.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Analysis of paraffin-embedded human colon cancer tissue using anti-Angiopoietin 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-JM71-34-Immunohistochemistry-Paraffin-NBP2-67154-img0002.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Analysis of paraffin-embedded human placenta tissue using anti-Angiopoietin 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-JM71-34-Immunohistochemistry-Paraffin-NBP2-67154-img0003.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody (JM71-34) [NBP2-67154] - Analysis of paraffin-embedded mouse liver tissue using anti-Angiopoietin 2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-JM71-34-Immunohistochemistry-Paraffin-NBP2-67154-img0004.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody [NBP2-15385] - Human colon carcinoma, using Angiopoietin 2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-15385-img0001.jpg "Immunohistochemistry-Paraffin: Angiopoietin-2 Antibody [NBP2-15385] - Human colon carcinoma, using Angiopoietin 2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Angiopoietin-2 Antibody [NBP2-15385] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-Western-Blot-NBP2-15385-img0003.jpg)
![Western Blot: Angiopoietin-2 Antibody [NBP2-15385] - HepG2 conditioned medium (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Angiopoietin 2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15385_rabbit-polyclonal-angiopoietin-2-antibody-western-blot-1142024823..jpg)
![Western Blot: Angiopoietin-2 Antibody (3H7) [NBP2-46520] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Angiopoietin 2.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-3H7-Western-Blot-NBP2-46520-img0003.jpg "Western Blot: Angiopoietin-2 Antibody (3H7) [NBP2-46520] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Angiopoietin 2.")
![Immunohistochemistry: Angiopoietin-2 Antibody (3H7) [NBP2-46520] - Analysis of Human lymphoma tissue](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-3H7-Immunohistochemistry-NBP2-46520-img0002.jpg)
![Immunohistochemistry: Angiopoietin-2 Antibody (3H7) [NBP2-46520] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-3H7-Immunohistochemistry-NBP2-46520-img0001.jpg)
![Western Blot: Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free [NBP2-70173] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Angiopoietin 2.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-OTI3H7-Azide-and-BSA-Free-Western-Blot-NBP2-70173-img0001.jpg "Western Blot: Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free [NBP2-70173] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Angiopoietin 2.")
![Immunohistochemistry: Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free [NBP2-70173] - Analysis of Human lymphoma tissue](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70173-img0002.jpg)
![Immunohistochemistry: Angiopoietin-2 Antibody (OTI3H7) - Azide and BSA Free [NBP2-70173] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70173-img0003.jpg)
![Western Blot: Angiopoietin-2 Antibody (117) [NBP2-89600] - Lane A: HepG2 Whole Cell Lysate Lane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:70 kDa](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-117-Western-Blot-NBP2-89600-img0002.jpg "Western Blot: Angiopoietin-2 Antibody (117) [NBP2-89600] - Lane A: HepG2 Whole Cell Lysate Lane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:57 kDa Observed band size:70 kDa")
![Immunoprecipitation: Angiopoietin-2 Antibody (117) [NBP2-89600] - Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 0.5 uL anti-Ang2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Ang2 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 57 kDaObserved band size: 57 kDa](http://images.novusbio.com/fullsize/Angiopoietin-2-Antibody-117-Immunoprecipitation-NBP2-89600-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Angiopoietin-2 Antibody (117) [NBP2-89600] - Staining of Human Ang2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89600 antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 549-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-89600_rabbit-monoclonal-angiopoietin-2-antibody-117-235202516335034.png)
![Western Blot: Angiopoietin-2 Antibody (S04-7E6) [NBP3-20029] - All lanes use NBP3-20029 at 1:6000 dilution for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-20029_rabbit-angiopoietin-2-mab-s04-7e6-234202415232518.jpg "Western Blot: Angiopoietin-2 Antibody (S04-7E6) [NBP3-20029] - All lanes use NBP3-20029 at 1:6000 dilution for 1 hour at room temperature.")
![Western Blot: Angiopoietin-2 Antibody (S04-7E6) - BSA Free [NBP3-20029] - All lanes use NBP3-20029 at 1: 6000 dilution for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-20029_rabbit-angiopoietin-2-mab-s04-7e6-103202523472615.jpg)
![SDS-Page: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-Angiopoietin-2-His-Protein-SDS-Page-NBP2-77507-img0001.jpg "SDS-Page: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![Bioactivity: Recombinant Human Angiopoietin-2 His Protein [NBP2-77507] - Human Angiopoietin-2 is coated at 5 ug/ml (100 ul/well) and can bind Human Tie-2 in a Functional ELISA assay](http://images.novusbio.com/fullsize/nbp2-77507_recombinant-human-angiopoietin-2-protein-1192023161301.jpg)
![Immunohistochemistry: Angiopoietin-2 Antibody (1F10) [NBP3-26509] - Image of Angiopoietin-2 Antibody (1F10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26509_rabbit-angiopoietin-2-mab-1f10-262202414471528.jpg "Immunohistochemistry: Angiopoietin-2 Antibody (1F10) [NBP3-26509] - Image of Angiopoietin-2 Antibody (1F10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Angiopoietin-2 Antibody (1F10) [NBP3-26509] - Image of Angiopoietin-2 Antibody (1F10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26509_rabbit-angiopoietin-2-mab-1f10-262202414471537.jpg)
![Western Blot: Angiopoietin 2 Overexpression Lysate (Adult Normal) [NBL1-07522] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Angiopoietin 2.](http://images.novusbio.com/fullsize/Angiopoietin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07522-img0002.jpg "Western Blot: Angiopoietin 2 Overexpression Lysate (Adult Normal) [NBL1-07522] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Angiopoietin 2.")