HAF109). A specific band was detected for Peroxiredoxin 6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Peroxiredoxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3490) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Peroxiredoxin 6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3490-SP | |||
HAF018). A specific band was detected for Peroxiredoxin 6 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Peroxiredoxin 6 Monoclonal Antibody (Catalog # MAB3490R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Peroxiredoxin 6 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3490R-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59436-50ul | |||
![Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone S1. Analysis of PRDX6 expression in MCF-7.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Western-Blot-H00009588-M01-img0002.jpg "Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone S1. Analysis of PRDX6 expression in MCF-7.")
![Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone 3A10-2A11. Analysis of PRDX6 expression in human liver.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Western-Blot-H00009588-M01-img0003.jpg)
![ELISA: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - Detection limit for recombinant GST tagged PRDX6 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-3A10-2A11-Sandwich-ELISA-H00009588-M01-img0001.jpg)
![Western Blot: Peroxiredoxin-6 Antibody [NBP2-19779] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-Western-Blot-NBP2-19779-img0002.jpg "Western Blot: Peroxiredoxin-6 Antibody [NBP2-19779] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Peroxiredoxin 6 Antibody (22E7) [NBP2-59436] - The recombinant human PRDX6 protein (50ng), Cell and Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 6 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant PRDX6 protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: HepG2 cell lysate Lane 5.: U87MG cell lysate Lane 6.: Ramos cell lysate](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-22E7-Western-Blot-NBP2-59436-img0001.jpg "Western Blot: Peroxiredoxin 6 Antibody (22E7) [NBP2-59436] - The recombinant human PRDX6 protein (50ng), Cell and Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin 6 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant PRDX6 protein Lane 2.: HeLa cell lysate Lane 3.: 293T cell lysate Lane 4.: HepG2 cell lysate Lane 5.: U87MG cell lysate Lane 6.: Ramos cell lysate")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 6 Antibody (22E7) [NBP2-59436] - Analysis of PRDX6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-22E7-Immunocytochemistry-Immunofluorescence-NBP2-59436-img0002.jpg)
![SDS-Page: Recombinant Human Peroxiredoxin 6 Protein [NBP1-30188] - Peroxiredoxin 6, 27.1 kDa (244aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Protein-SDS-Page-NBP1-30188-img0001.jpg "SDS-Page: Recombinant Human Peroxiredoxin 6 Protein [NBP1-30188] - Peroxiredoxin 6, 27.1 kDa (244aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
![Western Blot: Peroxiredoxin 6 Antibody [NBP1-55159] - HepG2 cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/Peroxiredoxin-6-Antibody-Western-Blot-NBP1-55159-img0002.jpg "Western Blot: Peroxiredoxin 6 Antibody [NBP1-55159] - HepG2 cell lysate, concentration 1.25ug/ml.")
![Western Blot: Peroxiredoxin 6 Antibody (7G1-R) [NBP3-32741] - Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (NBP3-32741) at 1/1,000 dilution.<br/><br/> Lane 1: PC-3M cell lysate<br/> Lane 2: RAW264.7 cell lysate<br/> Lane 3: PC-12 cell lysate<br/> Lane 4: Mouse testis tissue lysate<br/> Lane 5: Rat lung tissue lysate<br/> Lane 6: Rat liver tissue lysate<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 25 kDa<br/> Observed band size: 25 kDa<br/><br/> Exposure time: 1 minute 2 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32741) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32741_mouse-peroxiredoxin-6-mab-7g1-r-278202415295578.jpg "Western Blot: Peroxiredoxin 6 Antibody (7G1-R) [NBP3-32741] - Western blot analysis of Peroxiredoxin 6 on different lysates with Mouse anti-Peroxiredoxin 6 antibody (NBP3-32741) at 1/1,000 dilution.<br/><br/> Lane 1: PC-3M cell lysate<br/> Lane 2: RAW264.7 cell lysate<br/> Lane 3: PC-12 cell lysate<br/> Lane 4: Mouse testis tissue lysate<br/> Lane 5: Rat lung tissue lysate<br/> Lane 6: Rat liver tissue lysate<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 25 kDa<br/> Observed band size: 25 kDa<br/><br/> Exposure time: 1 minute 2 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32741) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western blot analysis on different lysates with NBP3-32741 at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat lung tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 27 kDa Exposure time: 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32741 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32741_-western-blot-639204594545020505.jpg)
![Immunoprecipitation:Peroxiredoxin 6 AntibodyNBP3-29960] -Detection of human PRDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRDX6 antibody NBP3-29960 (lot NBP3-29960-1) used for IP at 6 µg per reaction. PRDX6 was also immunoprecipitated by rabbit anti-PRDX6.. For blotting immunoprecipitated PRDX6, NBP3-29960 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29960_rabbit-peroxiredoxin-6-pab-3012202416204511.jpg "Immunoprecipitation:Peroxiredoxin 6 AntibodyNBP3-29960] -Detection of human PRDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRDX6 antibody NBP3-29960 (lot NBP3-29960-1) used for IP at 6 µg per reaction. PRDX6 was also immunoprecipitated by rabbit anti-PRDX6.. For blotting immunoprecipitated PRDX6, NBP3-29960 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation:Peroxiredoxin 6 AntibodyNBP3-29614] -Detection of human PRDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRDX6 antibody NBP3-29614 (lot NBP3-29614-1) used for IP at 6 µg per reaction. PRDX6 was also immunoprecipitated by rabbit anti-PRDX6.. For blotting immunoprecipitated PRDX6, NBP3-29614 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29614_rabbit-peroxiredoxin-6-pab-3012202420021.jpg "Immunoprecipitation:Peroxiredoxin 6 AntibodyNBP3-29614] -Detection of human PRDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PRDX6 antibody NBP3-29614 (lot NBP3-29614-1) used for IP at 6 µg per reaction. PRDX6 was also immunoprecipitated by rabbit anti-PRDX6.. For blotting immunoprecipitated PRDX6, NBP3-29614 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
