AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (
BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (
MAG999) and DNA was purified using chelating resin solution. The
B2Rpromoter was detected by standard PCR." class="big_lightbox">

AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (
BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (
MAG999) and DNA was purified using chelating resin solution. The
B2Rpromoter was detected by standard PCR." alt="D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (
AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (
BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (
MAG999) and DNA was purified using chelating resin solution. The
B2Rpromoter was detected by standard PCR." class="big_thumb" />