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![Western Blot: PAPSS1 Antibody [NBP2-97681] - Anti-PAPSS1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize2/PAPSS1-Antibody-Western-Blot-NBP2-97681-img0003.jpg "Western Blot: PAPSS1 Antibody [NBP2-97681] - Anti-PAPSS1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 72 kDa")
![Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody [NBP2-97681] - Immunofluorescence staining of PAPSS1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PAPSS1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize2/PAPSS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97681-img0002.jpg)
![Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-97681] - Immunochemical staining of human PAPSS1 in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize2/PAPSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-97681-img0004.jpg)
![Immunoprecipitation: PAPSS1 Antibody [NBP2-97681] - PAPSS1 was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate 4 ul anti-PAPSS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PAPSS1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize2/PAPSS1-Antibody-Immunoprecipitation-NBP2-97681-img0001.jpg)
![Western Blot: PAPSS1 Antibody [NBP1-33470] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Western Blot-NBP1-33470-img0005.jpg "Western Blot: PAPSS1 Antibody [NBP1-33470] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody [NBP1-33470] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33470-img0002.jpg)
![Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP1-33470] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Immunohistochemistry-Paraffin-NBP1-33470-img0003.jpg)
![Western Blot: PAPSS1 Antibody [NBP1-33470] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Western Blot-NBP1-33470-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody [NBP2-13730] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize2/PAPSS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13730-img0005.jpg "Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody [NBP2-13730] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.")
![Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-13730] - Staining of human smooth muscle shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Immunohistochemistry-Paraffin-NBP2-13730-img0006.jpg)
![Simple Western: PAPSS1 Antibody [NBP2-13730] - Lane view shows a specific band for PAPSS1 using 200 ug/mL of HepG2 cell lysate and 1:25 antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 65 kDa. Image from an internal validation.](http://images.novusbio.com/fullsize2/PAPSS1-Antibody-Simple-Western-NBP2-13730-img0008.jpg)
![Western Blot: PAPSS1 Antibody [NBP2-19692] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Western Blot-NBP2-19692-img0001.jpg "Western Blot: PAPSS1 Antibody [NBP2-19692] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-19692] - PAPSS1 antibody [N1C1] detects PAPSS1 protein at cytosol on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded gastric carcinoma. PAPSS1 antibody [N1C1] dilution: 1:500.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Immunohistochemistry-Paraffin-NBP2-19692-img0003.jpg)
![Immunohistochemistry-Paraffin: PAPSS1 Antibody [NBP2-19692] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Immunohistochemistry-Paraffin-NBP2-19692-img0002.jpg)
![Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05] - Analysis of PAPSS1 expression in transfected 293T cell line by PAPSS1 monoclonal antibody (M05), clone 1F4. Lane 1: PAPSS1 transfected lysatE (68.5 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody (1F4)-Western Blot-H00009061-M05-img0004.jpg "Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05] - Analysis of PAPSS1 expression in transfected 293T cell line by PAPSS1 monoclonal antibody (M05), clone 1F4. Lane 1: PAPSS1 transfected lysatE (68.5 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PAPSS1 Antibody (1F4) [H00009061-M05] - Analysis of monoclonal antibody to PAPSS1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize2/PAPSS1-Antibody-(1F4)-Immunocytochemistry-Immunofluorescence-H00009061-M05-img0003.jpg)
![Western Blot: PAPSS1 Antibody (1F4) [H00009061-M05] - Western blot analysis of PAPSS1 over-expressed 293 cell line, cotransfected with PAPSS1 Validated Chimera RNAi or non-transfected control. Blot probed with H00009061-M05. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize2/PAPSS1-Antibody-(1F4)-Western-Blot-H00009061-M05-img0002.jpg)
![Western Blot: PAPSS1 Antibody [NBP2-94650] - Analysis of extracts of various cell lines, using PAPSS1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize2/PAPSS1 Antibody-Western Blot-NBP2-94650-img0001.jpg "Western Blot: PAPSS1 Antibody [NBP2-94650] - Analysis of extracts of various cell lines, using PAPSS1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: PAPSS1 Overexpression Lysate (Adult Normal) [NBP2-07749] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAPSS1.](http://images.novusbio.com/fullsize/PAPSS1 Overexpression Lysate (Adult Normal)-Western Blot-NBP2-07749-img0001.jpg "Western Blot: PAPSS1 Overexpression Lysate (Adult Normal) [NBP2-07749] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAPSS1.")
![SDS-Page: Recombinant Human PAPSS1 Protein [NBP1-78889] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize2/Recombinant-Human-PAPSS1-Protein-SDS-Page-NBP1-78889-img0001.jpg "SDS-Page: Recombinant Human PAPSS1 Protein [NBP1-78889] - 15% SDS-PAGE (3ug)")