| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1669SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-126-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14061 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47439-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14060 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26237-50ul | |||
![Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NB100-1669-img0002.jpg "Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 preferably binds to nuclear Htt with long Qs. HDAC3 associates exclusively with nuclear inclusion bodies. E3 or N3 cells were fixed and stained with anti-HDAC3 antibodies and visualized by Alexa 546 conjugated secondary antibodies. Arrowheads: inclusion bodies with no HDAC3 signals associated. Arrows: HDAC3 signal-associated inclusion bodies. Bar=20 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111277), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1669-img0008.jpg)
![Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-1669] - Analysis human colon cancer using 1:50 conc. of HDAC3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of HDAC3 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-Paraffin-NB100-1669-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was used. Image objective 40x.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1669-img0004.jpg)
![Simple Western: HDAC3 Antibody [NB100-1669] - Lane view shows a specific band for HDAC3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Simple-Western-NB100-1669-img0005.jpg)
![Western Blot: HDAC3 Antibody - BSA Free [NB500-126] - Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK](http://images.novusbio.com/fullsize/HDAC3-Antibody---BSA-Free-Western-Blot-NB500-126-img0006.jpg "Western Blot: HDAC3 Antibody - BSA Free [NB500-126] - Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK")
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody - BSA Free [NB500-126] - HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.](http://images.novusbio.com/fullsize/HDAC3-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB500-126-img0004.jpg)
![Immunohistochemistry: HDAC3 Antibody - BSA Free [NB500-126] - Formalin-fixed, paraffin-embedded section of lymph node stained with 1:500 Anti-Histone Deacetylase 3 (HDAC3) antibody produced in Rabbit followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by ExtrAvidin(R)-Peroxidase](http://images.novusbio.com/fullsize/HDAC3-Antibody---BSA-Free-Immunohistochemistry-NB500-126-img0005.jpg)
![Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196] - SW480 xenograft, using HDAC3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-Paraffin-NBP1-31196-img0008.jpg "Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196] - SW480 xenograft, using HDAC3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Chromatin Immunoprecipitation: HDAC3 Antibody [NBP1-31196] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC3 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Chromatin-Immunoprecipitation-NBP1-31196-img0006.jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Analysis of on: A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP1-31196-img0009.jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC3 protein by 2 ug of HDAC3 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP1-31196-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC3 Antibody [NBP1-31196] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP1-31196-img0011.jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP1-31196-img0019.jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31196_rabbit-polyclonal-hdac3-antibody-western-blot-168202492658..jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31196_rabbit-polyclonal-hdac3-antibody-western-blot-168202492824..jpg)
![Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31196_rabbit-polyclonal-hdac3-antibody-910202420122079.jpg)
![HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:400.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-31196_rabbit-polyclonal-hdac3-antibody-immunocytochemistry-immunofluorescence-141120251838416.jpg)
![HDAC3 antibody [C3], C-term detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term (NBP1-31196) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-31196_rabbit-polyclonal-hdac3-antibody-immunocytochemistry-immunofluorescence-1411202518373815.jpg)
![Western Blot: HDAC3 Antibody [NBP2-16791] - HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0015.jpg "Western Blot: HDAC3 Antibody [NBP2-16791] - HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000.")
![Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP2-16791] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-Paraffin-NBP2-16791-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC3 Antibody [NBP2-16791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0004.jpg)
![Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NBP2-16791-img0014.jpg)
![HDAC3 antibody detects HDAC3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HDAC3 protein stained by HDAC3 antibody (NBP2-16791) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16791_rabbit-polyclonal-hdac3-antibody-immunocytochemistry-immunofluorescence-261120251962718.jpg)
![Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25497_rabbit-hdac3-mab-hl1749-azide-and-bsa-free-2120249383142.jpg "Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25497_rabbit-hdac3-mab-hl1749-azide-and-bsa-free-2120249403825.jpg)
![Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25497_rabbit-hdac3-mab-hl1749-azide-and-bsa-free-212024941412.jpg)
![Western Blot: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25497_rabbit-hdac3-mab-hl1749-azide-and-bsa-free-2120249393544.jpg)
![Immunohistochemistry-Paraffin: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - HDAC3 antibody [HL1749] detects HDAC3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. HDAC3 stained by HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25497_rabbit-hdac3-mab-hl1749-azide-and-bsa-free-2120249362779.jpg)
![Immunohistochemistry-Paraffin: HDAC3 Antibody (HL1749) - Azide and BSA Free [NBP3-25497] - HDAC3 antibody [HL1749] detects HDAC3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. HDAC3 stained by HDAC3 antibody [HL1749] (NBP3-25497) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25497_rabbit-hdac3-mab-hl1749-azide-and-bsa-free-2120249425424.jpg)
![Western Blot: HDAC3 Antibody [NB100-79798] - A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 mcg), Ramos (R; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC3 Antibody NB100-79798 used at 0.02 mcg/ml for WB (A) and at 3 mcg/mg lysate for IP (B).](http://images.novusbio.com/fullsize/HDAC3-Antibody-Western-Blot-NB100-79798-img0002.jpg "Western Blot: HDAC3 Antibody [NB100-79798] - A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 mcg), Ramos (R; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC3 Antibody NB100-79798 used at 0.02 mcg/ml for WB (A) and at 3 mcg/mg lysate for IP (B).")
![Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-79798] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HDAC3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-Paraffin-NB100-79798-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-3C9) [NBP3-13810] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/HDAC3-Antibody-PCRP-HDAC3-3C9-Immunocytochemistry-Immunofluorescence-NBP3-13810-img0002.jpg "Immunocytochemistry/Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-3C9) [NBP3-13810] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).")
![Flow Cytometry: HDAC3 Antibody (PCRP-HDAC3-3C9) [NBP3-13810] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC3 antibody (PCRP-HDAC3-3C9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/HDAC3-Antibody-PCRP-HDAC3-3C9-Flow-Cytometry-NBP3-13810-img0001.jpg)
![Protein Array: HDAC3 Antibody (PCRP-HDAC3-3C9) [NBP3-13810] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC3 Antibody (PCRP-HDAC3-3C9).](http://images.novusbio.com/fullsize/HDAC3-Antibody-PCRP-HDAC3-3C9-Protein-Array-NBP3-13810-img0004.jpg)
![Immunohistochemistry: HDAC3 Antibody [NBP2-47439] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/HDAC3-Antibody-Immunohistochemistry-NBP2-47439-img0002.jpg "Immunohistochemistry: HDAC3 Antibody [NBP2-47439] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.")
![Immunocytochemistry/Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Antibody (PCRP-HDAC3-2D4) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/HDAC3-Antibody-PCRP-HDAC3-2D4-Immunocytochemistry-Immunofluorescence-NBP3-13809-img0002.jpg "Immunocytochemistry/Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Antibody (PCRP-HDAC3-2D4) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).")
![Flow Cytometry: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC3 antibody (PCRP-HDAC3-2D4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/HDAC3-Antibody-PCRP-HDAC3-2D4-Flow-Cytometry-NBP3-13809-img0001.jpg)
![Protein Array: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC3 Antibody (PCRP-HDAC3-2D4).](http://images.novusbio.com/fullsize/HDAC3-Antibody-PCRP-HDAC3-2D4-Protein-Array-NBP3-13809-img0004.jpg)
![Immunohistochemistry-Paraffin: HDAC3 Antibody (RM439) [NBP3-23463] - Immunohistochemical staining of formalin fixed and paraffin embedded human brain cancer tissue sections using Clone RM439) at 1:200 dilution.](http://images.novusbio.com/fullsize/nbp3-23463_rabbit-hdac3-mab-24620241245911.png "Immunohistochemistry-Paraffin: HDAC3 Antibody (RM439) [NBP3-23463] - Immunohistochemical staining of formalin fixed and paraffin embedded human brain cancer tissue sections using Clone RM439) at 1:200 dilution.")
![Immunohistochemistry-Paraffin: HDAC3 Antibody (RM439) [NBP3-23463] - Immunohistochemical staining of formalin fixed and paraffin embedded human lung squamous carcinoma tissue sections using Clone RM439) at 1:200 dilution.](http://images.novusbio.com/fullsize/nbp3-23463_rabbit-hdac3-mab-226202422174415.png)
![Western Blot: HDAC3 Antibody (RM439) [NBP3-23463] - Western Blot of Jurkat cell lysate using RM439 at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-23463_rabbit-hdac3-mab-western-blot-189202523435620.png%252522)
![Immunohistochemistry: HDAC3 Antibody (4D4) [NBP3-26237] - Image of HDAC3 Antibody (4D4) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26237_rabbit-hdac3-mab-4d4-26220241510611.jpg "Immunohistochemistry: HDAC3 Antibody (4D4) [NBP3-26237] - Image of HDAC3 Antibody (4D4) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: HDAC3 Antibody (4D4) [NBP3-26237] - Image of HDAC3 Antibody (4D4) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26237_rabbit-hdac3-mab-4d4-2722024856314.jpg)
![Western Blot: HDAC3 Antibody (4D4) [NBP3-26237] - Positive Western Blot detected in: Hela whole cell lysate, HL60 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate, 293T whole cell lysate. All lanes: HDAC3 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 49, 50 kDa Observed band size: 49 kDa](http://images.novusbio.com/fullsize/nbp3-26237_rabbit-hdac3-mab-4d4-282202411442645.jpg)
![SDS-Page: Recombinant Human HDAC3 Protein [H00008841-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00008841-P01-1.jpg "SDS-Page: Recombinant Human HDAC3 Protein [H00008841-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")