HAF016). A specific band was detected for IQGAP1 at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line, and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5069) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP1 at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5069-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21443-25ul | |||
![Immunocytochemistry/Immunofluorescence: IQGAP1 Antibody (2C5) [H00008826-M01] - Analysis of monoclonal antibody to IQGAP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Immunocytochemistry-Immunofluorescence-H00008826-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: IQGAP1 Antibody (2C5) [H00008826-M01] - Analysis of monoclonal antibody to IQGAP1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Immunohistochemistry-Paraffin-H00008826-M01-img0008.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 monoclonal antibody (M01), clone 2C5 Analysis of IQGAP1 expression in C32.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Western-Blot-H00008826-M01-img0010.jpg)
![Sandwich ELISA: IQGAP1 Antibody (2C5) [H00008826-M01] - Detection limit for recombinant GST tagged IQGAP1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-2C5-Sandwich-ELISA-H00008826-M01-img0009.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - indicates that IQGAP1 mRNA & protein levels in HO-8910PM-shIQGAP1, HO-8910PM-shRNA negative & un-transfected HO-8910PM cells were determined by RT-PCR & Western blot analysis, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19036171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415345311.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - shows that IQGAP1 expression & cell invasive phenotype in ovarian cancer cell lines. (a) RT-PCR analysis for IQGAP1 using total RNA form HO-8910, SK-OV-3 & HO-8910PM cells. (b) Western blot analysis for IQGAP1 in whole-cell lysates from the indicated cell lines. (c) High IQGAP1 expression associated with enhanced invasive potential of ovarian cancer cells. HO-8910, SK-OV-3 & HO-8910PM cells were seeded onto a Matrigel-coated invasion chamber & the number of invading cells was determined as described before. *, P < 0.05 versus SK-OV-3 or HO-8910 cells. (d) High IQGAP1 expression correlated with enhanced migratory potential of ovarian cancer cells. HO-8910, SK-OV-3 & HO-8910PM cells were seeded onto a Boyden chamber & the number of migrating cells was determined as described before. *, P < 0.05 versus SK-OV-3 or HO-8910 cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19036171), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415483611.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 induces the epithelial-to-mesenchymal transition, invasiveness, & proliferation of endometrial cancer (EC) cellsA. Reverse transcription quantitative PCR (qPCR) analysis of IQGAP1 mRNA in the immortalized human endometrial cell line EM & the EC cells HEC-1, HEC-50, & HEC-50-HI (HI). The results are presented as the fold-change in expression compared to the EM cells. B. Western blot analysis of the IQGAP1 protein in EC cells. C, E. The expression of IQGAP1, E-cadherin, & N-cadherin proteins in HI cells transfected with control (Ctr) or IQGAP1 siRNA (C) & in HEC-1 cells expressing either the control or IQGAP1 vector (E). D, F. Phase-contrast microscopy shows the morphology of HI cells transfected with control or IQGAP1 siRNA (D) & HEC-1 cells transfected with the control or IQGAP1 vector (F). G-I. Detection of migration (G), invasion (H), & proliferation (I) in HI & HEC-1 cells after the indicated transfection. J, K. qPCR analysis of ZO-1, CK-18, & Vimentin expression in HI (J) & HEC-1 (K) cells, transfected as indicated. L. Representative images from the invasion assays. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-310202415482215.jpg)
![Western Blot: IQGAP1 Antibody (2C5) [H00008826-M01] - IQGAP1 induces the epithelial-to-mesenchymal transition, invasiveness, & proliferation of endometrial cancer (EC) cellsA. Reverse transcription quantitative PCR (qPCR) analysis of IQGAP1 mRNA in the immortalized human endometrial cell line EM & the EC cells HEC-1, HEC-50, & HEC-50-HI (HI). The results are presented as the fold-change in expression compared to the EM cells. B. Western blot analysis of the IQGAP1 protein in EC cells. C, E. The expression of IQGAP1, E-cadherin, & N-cadherin proteins in HI cells transfected with control (Ctr) or IQGAP1 siRNA (C) & in HEC-1 cells expressing either the control or IQGAP1 vector (E). D, F. Phase-contrast microscopy shows the morphology of HI cells transfected with control or IQGAP1 siRNA (D) & HEC-1 cells transfected with the control or IQGAP1 vector (F). G-I. Detection of migration (G), invasion (H), & proliferation (I) in HI & HEC-1 cells after the indicated transfection. J, K. qPCR analysis of ZO-1, CK-18, & Vimentin expression in HI (J) & HEC-1 (K) cells, transfected as indicated. L. Representative images from the invasion assays. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00008826-m01_mouse-monoclonal-iqgap1-antibody-2c5-31020241548516.jpg)
![Western Blot: IQGAP1 Antibody [NBP1-06529] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Western-Blot-NBP1-06529-img0011.jpg "Western Blot: IQGAP1 Antibody [NBP1-06529] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IQGAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-06529] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IQGAP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-06529-img0009.jpg)
![Immunoprecipitation: IQGAP1 Antibody [NBP1-06529] - Detection of human IQGAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IQGAP1 antibody NBP1-06529 (lot NBP1-06529-2) used for IP at 3 ug per reaction. IQGAP1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06529-1). For blotting immunoprecipitated IQGAP1, NBP1-06529 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunoprecipitation-NBP1-06529-img0010.jpg)
![Immunohistochemistry: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/4,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32479) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32479_rabbit-iqgap1-mab-je50-66-29820241492650.jpg "Immunohistochemistry: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/4,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32479) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Immunocytochemistry analysis of HeLa cells labeling IQGAP1 with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IQGAP1 antibody (NBP3-32479) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32479_rabbit-iqgap1-mab-je50-66-29202410484868.jpg)
![Flow Cytometry: IQGAP1 Antibody (JE50-66) [NBP3-32479] - Flow cytometric analysis of HeLa cells labeling IQGAP1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32479, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32479_rabbit-iqgap1-mab-je50-66-29202416372144.jpg)
![Western blot analysis on different lysates with NBP3-32479 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 189 kDa Observed band size: 189 kDa Exposure time: 1 minute 40 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32479 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32479_-western-blot-639204597146551409.jpg)
![Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Western-Blot-NBP1-83143-img0008.jpg "Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining in human endometrium and skeletal muscle tissues using anti-IQGAP1 antibody. Corresponding IQGAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83143-img0014.jpg)
![Western Blot: IQGAP1 Antibody [NBP1-83143] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Western-Blot-NBP1-83143-img0007.jpg)
![Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining of human endometrium shows high expression.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83143-img0012.jpg)
![Immunohistochemistry-Paraffin: IQGAP1 Antibody [NBP1-83143] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83143-img0013.jpg)
![Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Western blot analysis of extracts of various cell lines, using IQGAP1 Rabbit mAb (NBP3-16879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-7I6X1-Western-Blot-NBP3-16879-img0002.jpg "Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Western blot analysis of extracts of various cell lines, using IQGAP1 Rabbit mAb (NBP3-16879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Immunohistochemistry of paraffin-embedded human liver using IQGAP1 Rabbit mAb (NBP3-16879) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-7I6X1-Immunohistochemistry-Paraffin-NBP3-16879-img0001.jpg)
![SDS-Page: IQGAP1 Partial Recombinant Protein [H00008826-Q01]](http://images.novusbio.com/fullsize/IQGAP1-Partial-Recombinant-Protein-SDS-Page-H00008826-Q01-img0001.jpg "SDS-Page: IQGAP1 Partial Recombinant Protein [H00008826-Q01]")
![Western Blot: IQGAP1 Antibody [NBP1-51980] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/IQGAP1-Antibody-Western-Blot-NBP1-51980-img0002.jpg "Western Blot: IQGAP1 Antibody [NBP1-51980] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: IQGAP1 Overexpression Lysate (Adult Normal) [NBP2-07941] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IQGAP1.](http://images.novusbio.com/fullsize/IQGAP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07941-img0001.jpg "Western Blot: IQGAP1 Overexpression Lysate (Adult Normal) [NBP2-07941] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IQGAP1.")
