Products Related to Gene-ID: 80380

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="PD-L2/B7-DC was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB1224) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="PD-L2 was detected in perfusion fixed frozen sections of mouse thymus using 15 µg/mL Goat Anti-Mouse PD-L2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1022) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF017). Specific bands were detected for PD-L2/B7-DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for PD-L2/B7-DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-L2/B7-DC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1224) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-L2/B7-DC at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.274 uM. " class="big_lightbox"> 8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.274 uM. " class="big_thumb" />

F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either mouse PD-L2/B7-DC (filled histogram) or irrelevant transfectants (open histogram) was stained with Rat Anti-Mouse PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB1022), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

1086-PD) binds with an ED₅₀ of 0.08-0.4 µg/mL." class="big_lightbox"> 1086-PD) binds with an ED₅₀ of 0.08-0.4 µg/mL." alt="When Recombinant Human PD-L2/B7-DC is immobilized at 1 µg/mL, Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) binds with an ED₅₀ of 0.08-0.4 µg/mL." class="big_thumb" />

AVI8986) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human PD-L2 His protein (Catalog # 9075-PL) was measured at a concentration range between 5.07 nM and 2.60 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.173 µM." class="big_lightbox"> AVI8986) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human PD-L2 His protein (Catalog # 9075-PL) was measured at a concentration range between 5.07 nM and 2.60 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.173 µM." class="big_thumb" />