| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-56893SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27739-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32477-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-56893SS | |||
| BSA Free | NB110-56893 | |||
![Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Western-Blot-NB110-56893-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody [NB110-56893] - Detection of ATG9A protein in HEK293 lysates using NB110-56893. (1) siRNA ATG9A knockdown and (2) wildtype ATG9A.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody [NB110-56893] - After stimulation with the NOD1 ligand, macrophages increased their expression of autophagy proteins Atg9, LC3 and IRGM. Alveolar macrophages (AMs), monocyte-derived macrophages (MDMs) and monocytes (MNs) were incubated in the presence of 5 ug/ml of Tri-DAP for 24 h. Cells were pre-incubated with Rip2/p38 inhibitor SB203580 (SB) for 30 min prior to Tri-DAP stimulation to block NOD1-mediated responses, as indicated. Atg9 and LC3 proteins were measured in the cytosolic fractions by western blot analysis. Image collected and cropped by CiteAb from the following publication (//bmcpulmmed.biomedcentral.com/articles/10.1186/1471-2466-14-152), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Western-Blot-NB110-56893-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NB110-56893] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-ATG9A at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunocytochemistry-Immunofluorescence-NB110-56893-img0008.jpg)
![Immunohistochemistry: ATG9A Antibody [NB110-56893] - Staining in mouse intestine.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunohistochemistry-NB110-56893-img0005.jpg)
![Flow Cytometry: ATG9A Antibody [NB110-56893] - An intracellular stain was performed on Hela Cells with NB110-56893 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).](http://images.novusbio.com/fullsize/ATG9A-Antibody-Flow-Cytometry-NB110-56893-img0007.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of chloroquine, spautin-1 & MG132 on ATG protein levels in A549 cells. (A) A549 cells were treated with 50 μM chloroquine, 10 μM spautin-1 or 5 μM MG132 in the presence or absence of 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3, mean±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415371977.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - CD133 promoted glucose uptake.A. Levels of glucose uptake in Huh-7con & Huh-7sh133 cells. Mean fluorescence intensity of 2-NBDG uptake was measured by FASC analysis after incubating for 6 h. B. Time course of 2-NBDG uptake in these two cell lines. Statistical significance indicated as *p<0.05; **p<0.01. C. CD133 or empty vector was transfected respectively into LM3 cells. Glucose uptake was measured at the indicated times upon the medium added with 100 µM 2-NBDG. *p<0.05. D. Detection of relative activities of signaling pathways in Huh-7con & Huh-7sh133 cells by Western blotting. con: Huh-7con cells; sh: Huh-7sh133 cells. E. Detection of autophagic genes in Huh-7con & Huh-7sh133 cells by Western blotting. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437259), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415382433.jpg)
![Western Blot: ATG9A Antibody - BSA Free [NB110-56893] - The effect of TGF beta 1 on ATG protein levels & LC3B lipidation in A549 cells. (A) A549 cells were treated with 250 pM TGF beta 1 for 24 h. Cells were lysed & subjected to SDS-PAGE & immunoblotting anti-ATG3, anti-ATG5, anti-ATG7, anti-ATG9, anti-ATG12, anti-ATG12-ATG5 complex, anti-ATG16L1, anti-BECN1, anti-ULK1, anti-LC3B, anti-P-Smad2, anti-Smad2 & anti-GAPDH (loading control) antibodies. (B) The steady state levels of ATG3, ATG5, ATG7, ATG9, ATG12, ATG12-ATG5, ATG16L1, BECN1, ULK1, & LC3B were quantitated using QuantityOne software & graphed (n=3±s.e.m.). Significance is indicated as *=P<0.05, **P<0.01 & ****=P<0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33168592), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-56893_rabbit-polyclonal-atg9a-antibody-310202415384151.jpg)
![Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0002.jpg "Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.")
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Staining of PFA fixed HEK293 cells labelled with hamster anti-Atg9 (Left) and rabbit anti-Atg9.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Immunofluorescence-NBP2-50109-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of siRNA knockdown of human Atg9 (si) in Hela and Hek293 cells compared to siControl siRNA (siC) reveals specicity of hamster anti-Atg9 antibody.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG9A Antibody (SR3028) [NBP3-27739] - Immunohistochemical analysis of paraffin-embedded human lung cancer](http://images.novusbio.com/fullsize/nbp3-27739_rabbit-atg9a-mab-sr3028-752024939136.jpg "Immunohistochemistry-Paraffin: ATG9A Antibody (SR3028) [NBP3-27739] - Immunohistochemical analysis of paraffin-embedded human lung cancer")
![Western Blot: ATG9A Antibody (SR3028) [NBP3-27739] - Western blot analysis of ATG9A expression in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27739_rabbit-atg9a-mab-sr3028-7520241015115.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG9A Antibody (SR3028) [NBP3-27739] - Immunofluorescent analysis of Hepg2 cells](http://images.novusbio.com/fullsize/nbp3-27739_rabbit-atg9a-mab-sr3028-75202410133477.jpg)
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NBP2-32477] - Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32477-img0008.jpg "Immunocytochemistry/Immunofluorescence: ATG9A Antibody [NBP2-32477] - Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.")
![Immunohistochemistry-Paraffin: ATG9A Antibody [NBP2-32477] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Immunohistochemistry-Paraffin-NBP2-32477-img0009.jpg)
![Immunoprecipitation:[NBP3-30437]-Detection of human by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated , was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30437_rabbit-atg9a-pab-2312025128818.jpg "Immunoprecipitation:[NBP3-30437]-Detection of human by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated , was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: ATG9A Overexpression Lysate (Adult Normal) [NBP2-09258] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG9A.](http://images.novusbio.com/fullsize/ATG9A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09258-img0001.jpg "Western Blot: ATG9A Overexpression Lysate (Adult Normal) [NBP2-09258] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG9A.")
![Western Blot: ATG9A Overexpression Lysate (Adult Normal) [NBP2-08449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG9A.](http://images.novusbio.com/fullsize/ATG9A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08449-img0001.jpg "Western Blot: ATG9A Overexpression Lysate (Adult Normal) [NBP2-08449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG9A.")
![Western Blot: ATG9A Overexpression Lysate (Adult Normal) [NBP2-06353] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG9A.](http://images.novusbio.com/fullsize/ATG9A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06353-img0001.jpg "Western Blot: ATG9A Overexpression Lysate (Adult Normal) [NBP2-06353] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATG9A.")
![Immunoprecipitation: ATG9A Antibody - Azide and BSA Free [NBP3-26074] - ATG9A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 uL NBP3-26074 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-26074, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 94 kDa](http://images.novusbio.com/fullsize/nbp3-26074_rabbit-atg9a-pab-azide-and-bsa-free-13620242234114.png "Immunoprecipitation: ATG9A Antibody - Azide and BSA Free [NBP3-26074] - ATG9A was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 uL NBP3-26074 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-26074, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 94 kDa")