| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54539-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21161-25ul | |||
| Azide and BSA Free | NBP3-44319 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54486-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08274 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08275 | |||
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs). Schematic description of the two-stage protocol applied to iPSC-renal commitment. Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. Pictured are the IM and MM marker expressions as Wt1, Pax8, Pax2, Six2 and Sall1. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32440-img0020.jpg "Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs). Schematic description of the two-stage protocol applied to iPSC-renal commitment. Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. Pictured are the IM and MM marker expressions as Wt1, Pax8, Pax2, Six2 and Sall1. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Pax8 immunoreactivity in canine renal carcinoma. Primary antibody incubated with tissue for 1h at room temperature. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0021.jpg)
![Chromatin Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of PAX8 antibody The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.](http://images.novusbio.com/fullsize/PAX8-Antibody-Chromatin-Immunoprecipitation-NBP1-32440-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32440-img0011.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Rat thyroid gland. PAX8 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0015.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - DLD1 xenograft . PAX8 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0016.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Mouse liver. PAX8 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0018.jpg)
![Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunoprecipitation-NBP1-32440-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP1-32440] - Differentiation of human iPSC clone IV towards renal commitment.(a) Representative immunofluorescence images of co-staining for Lhx1/Osr1 up to day 6. (b) Images of co-staining of Pax2/Six2, Pax8/Six2 & Six2/Wt1 from day 6 to 12. (c) Expression of renal progenitor markers such as CD24, Claudin1 & GGT1 from day 0 to 19. Nuclei are stained with DAPI (blue). Scale bars: 50 μm (a, b), 20 μm (c). (d) Gene expression analysis for renal progenitor markers at different points in time. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-310202415345341.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - PDX model establishment from ccRCC injection. a Hematoxylin & Eosin staining of human tumor engrafted in murine models. A epresentative image of kidney PDX reported. Microscope Nikon Eclipse E1000 10X & 20X (b) Graph reporting % of patients who engrafted when orthotopically injected in mice & distributed following grading (3 G1; 7 G2; 13 G3; 7 G4). Eight mice for each patient injected & all 18 tumors on 30 which evaluated as engrafted developed tumor masses on ≥ of 6 mice. Tumors declared unable to engraft did not produce, at all, tumor masses. c Representative images (1 × 0.63) of PDXs excised 90 days after injection by Stereomicroscope (Olympus SZX10,XCX50). One representative image for G2, G3 & G4 types reported. d Representative images (1 × 0.63 & 1.25) of aberrant neo-angiogenesis formation in PDXs by Stereomicroscope (Olympus SZX10, XCX50 camera). e Hematoxylin & Eosin staining of PDXs versus parental primary patients. One representative tumor for each grade (G2, G3, G4) reported. Staining executed on OCT frozen samples. f Hematoxylin & Eosin & anti-PAX8, CD10, Vimentin & EMA staining reported on formalin-fixed & paraffin- embedded parental primary, metastatic tissues & PDXs. Primary, metastatic & PDX tissues obtained & shown from one representative patient. Microscope Nikon Eclipse 55i, magnification 20×. g Histogram showing number of engrafting tumor populations evaluated over 30 injected patient samples & correlated w/ patient recurrence frequency calculated as development of metastases after surgery. Orange color represents recurrent (n = 7) & metastatic (n = 3) patients in engrafted group (n = 18). Pink color represents recurrent patients (n = 2) in non-engrafted group (n = 12) for a total of 30 injected samples Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/30185225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-310202416165612.jpg)
![Western Blot: PAX8 Antibody [NBP1-32440] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (NBP1-32440) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-910202420134072.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - PAX8 antibody detects PAX8 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.PAX8 stained by PAX8 antibody (NBP1-32440) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-910202419593589.jpg)
![Western Blot: PAX8 Antibody [NBP1-32440] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (NBP1-32440) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-9102024201466.jpg)
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0011.jpg "Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.")
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunocytochemistry-Immunofluorescence-NBP2-29903-img0005.jpg)
![Immunohistochemistry: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Staining in mouse thyroid tissue at pre-tumor stage (red). Dilution is 1:100. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunocytochemistry-NBP2-29903-img0012.jpg)
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 60ug of SKOV-3, COS7, and negative control HeLa whole cell lysates, and 10ul of PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0009.jpg)
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 25ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane), and 10ul PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0010.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of mouse kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0006.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0007.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0008.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of HEK293 lysate overexpressing PAX8 (right lane) or empty vector control (left lane) and 10ul PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0011.jpg "Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of HEK293 lysate overexpressing PAX8 (right lane) or empty vector control (left lane) and 10ul PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP2-29907] - Analysis showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29907-img0007.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 75 ug of various whole cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0009.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 60ug of COS7 whole cell lysate (left lane), negative control HeLa lysate (right lane), and 10ul PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0010.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP2-29907] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 polyclonal antibody at a dilution of 1:400 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29907-img0006.jpg)
![Immunoprecipitation: PAX8 Antibody [NBP2-29907] - Analysis of PAX8 was performed using COS7 whole cell lysate. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of a PAX8 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with a PAX8 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C. Membranes were washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunoprecipitation-NBP2-29907-img0008.jpg)
![Western Blot: PAX8 Antibody (PAX8/1492) [NBP2-53371] - Western Blot Analysis (A) Recombinant Protein (B) Raji Cell Lysate Using PAX8 Monoclonal Antibody (PAX8/1492).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1492-Western-Blot-NBP2-53371-img0004.jpg "Western Blot: PAX8 Antibody (PAX8/1492) [NBP2-53371] - Western Blot Analysis (A) Recombinant Protein (B) Raji Cell Lysate Using PAX8 Monoclonal Antibody (PAX8/1492).")
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/1492) [NBP2-53371] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with PAX8 Monoclonal Antibody (PAX8/1492).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1492-Immunohistochemistry-Paraffin-NBP2-53371-img0003.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/1492) [NBP2-53371] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with PAX8 Monoclonal Antibody (PAX8/1492).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1492-Immunohistochemistry-Paraffin-NBP2-53371-img0001.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/1492) [NBP2-53371] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with PAX8 Monoclonal Antibody (PAX8/1492).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1492-Immunohistochemistry-Paraffin-NBP2-53371-img0002.jpg)
![Protein Array: PAX8 Antibody (PAX8/1492) [NBP2-53371] - Analysis of Protein Array containing >19,000 full-length human proteins using PAX8 Antibody (PAX8/1492) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1492-Protein-Array-NBP2-53371-img0006.jpg)
![Western Blot: PAX8 Antibody (PAX8/1491) [NBP2-53260] - Western Blot Analysis (A) Recombinant Protein (B) Raji Cell Lysate Using PAX8 Monoclonal Antibody (PAX8/1491).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1491-Western-Blot-NBP2-53260-img0001.jpg "Western Blot: PAX8 Antibody (PAX8/1491) [NBP2-53260] - Western Blot Analysis (A) Recombinant Protein (B) Raji Cell Lysate Using PAX8 Monoclonal Antibody (PAX8/1491).")
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/1491) [NBP2-53260] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with PAX8 Antibody (PAX8/1491).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1491-Immunohistochemistry-Paraffin-NBP2-53260-img0003.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/1491) [NBP2-53260] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with PAX8 Antibody (PAX8/1491).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1491-Immunohistochemistry-Paraffin-NBP2-53260-img0002.jpg)
![Protein Array: PAX8 Antibody (PAX8/1491) [NBP2-53260] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PAX8 Antibody (PAX8/1491) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1491-Protein-Array-NBP2-53260-img0004.jpg)
![Western Blot: PAX8 Antibody (6G5-R) [NBP3-32719] - Western blot analysis of PAX8 on different lysates with Mouse anti-PAX8 antibody (NBP3-32719) at 1/1,000 dilution. Lane 1: NIH:OVCAR-3 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32719) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32719_mouse-pax8-mab-6g5-r-278202416331286.jpg "Western Blot: PAX8 Antibody (6G5-R) [NBP3-32719] - Western blot analysis of PAX8 on different lysates with Mouse anti-PAX8 antibody (NBP3-32719) at 1/1,000 dilution. Lane 1: NIH:OVCAR-3 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32719) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PAX8 Antibody (6G5-R) [NBP3-32719] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-PAX8 antibody (NBP3-32719) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32719) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32719_mouse-pax8-mab-6g5-r-298202414262698.jpg)
![Immunocytochemistry/ Immunofluorescence: PAX8 Antibody (6G5-R) [NBP3-32719] - Immunocytochemistry analysis of NIH:OVCAR-3 (positive) and HeLa (negative) labeling PAX8 with Mouse anti-PAX8 antibody (NBP3-32719) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PAX8 antibody (NBP3-32719) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32719_mouse-pax8-mab-6g5-r-2920241023427.jpg)
![Western Blot: PAX8 Antibody (PAX8/1491 + PAX8/1492) - Azide and BSA Free [NBP3-08274] - Western Blot Analysis (A) Recombinant Protein (B) Raji cell lysate using PAX8Monoclonal Antibody (PAX8/1491 + PAX8/1492).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1491-+-PAX8-1492-Azide-and-BSA-Free-Western-Blot-NBP3-08274-img0002.jpg "Western Blot: PAX8 Antibody (PAX8/1491 + PAX8/1492) - Azide and BSA Free [NBP3-08274] - Western Blot Analysis (A) Recombinant Protein (B) Raji cell lysate using PAX8Monoclonal Antibody (PAX8/1491 + PAX8/1492).")
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/1491 + PAX8/1492) - Azide and BSA Free [NBP3-08274] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with PAX8 Monoclonal Antibody (PAX8/1491 + PAX8/1492).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1491-+-PAX8-1492-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08274-img0001.jpg)
![Western Blot: PAX8 Antibody (PAX8/1491 + PAX8/1492) [NBP3-07355] - Western Blot Analysis (A) Recombinant Protein (B) Raji cell lysate using PAX8Monoclonal Antibody (PAX8/1491 + PAX8/1492).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1491-+-PAX8-1492-Western-Blot-NBP3-07355-img0002.jpg "Western Blot: PAX8 Antibody (PAX8/1491 + PAX8/1492) [NBP3-07355] - Western Blot Analysis (A) Recombinant Protein (B) Raji cell lysate using PAX8Monoclonal Antibody (PAX8/1491 + PAX8/1492).")
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/1491 + PAX8/1492) [NBP3-07355] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with PAX8 Monoclonal Antibody (PAX8/1491 + PAX8/1492).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-1491-+-PAX8-1492-Immunohistochemistry-Paraffin-NBP3-07355-img0001.jpg)
![Western Blot: PAX8 Antibody [NB100-1065] - Staining of Human Kidney (A) and HeLa cell (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NB100-1065-img0008.jpg "Western Blot: PAX8 Antibody [NB100-1065] - Staining of Human Kidney (A) and HeLa cell (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PAX8 Antibody [NB100-1065] - Staining of paraffin embedded Human Kidney Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NB100-1065-img0010.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/2774R) [NBP3-07356] - Formalin-fixed, paraffin-embedded human Thyroid stained with PAX8 Recombinant Rabbit Monoclonal Antibody (PAX8/2774R).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-2774R-Immunohistochemistry-Paraffin-NBP3-07356-img0002.jpg "Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/2774R) [NBP3-07356] - Formalin-fixed, paraffin-embedded human Thyroid stained with PAX8 Recombinant Rabbit Monoclonal Antibody (PAX8/2774R).")
![Immunohistochemistry-Paraffin: PAX8 Antibody (PAX8/2774R) [NBP3-07356] - Formalin-fixed, paraffin-embedded human Kidney stained with PAX8 Recombinant Rabbit Monoclonal Antibody (PAX8/2774R).](http://images.novusbio.com/fullsize/PAX8-Antibody-PAX8-2774R-Immunohistochemistry-Paraffin-NBP3-07356-img0001.jpg)
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![Immunohistochemistry-Paraffin: PAX8 Antibody (RM436) [NBP3-18831] - Immunohistochemical staining of formalin fixed and paraffin embedded human thyroid tissue section using RM436 at a 1:400 dilution.](http://images.novusbio.com/fullsize/nbp3-18831_rabbit-pax8-mab-rm436-immunohistochemistry-paraffin-18920252337402.png)