HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14-3-3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">
HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14-3-3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromoÂcytoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14-3-3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14-3-3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromoÂcytoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/ Mouse/Rat 14-3-3e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (2 ng/lane) were included. Specific bands for 14-3-3 epsilon was detected at approximately 26 and 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-89827] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-89827-img0009.jpg "Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-89827] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.")
![Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3 epsilon Antibody (CPTC-YWHAE-1).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Western-Blot-NBP2-79810-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3 epsilon Antibody (CPTC-YWHAE-1).")
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Formalin-fixed, paraffin-embedded human Testis stained with 14-3-3 epsilon Antibody (CPTC-YWHAE-1).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Immunohistochemistry-Paraffin-NBP2-79810-img0001.jpg)
![Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. 14-3-3 epsilon Antibody (NBP2-79810) primary antibody at 1:1000, 4C, overnight. Wb image submitted by a verified customer review.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Western-Blot-NBP2-79810-img0004.jpg)
![Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) [NBP2-79810] - Analysis of Protein Array containing more than 19,000 full-length human proteins using 14-3-3 epsilon Antibody (CPTC-YWHAE-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-CPTC-YWHAE-1-Protein-Array-NBP2-79810-img0002.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-32695-img0017.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-32695] - Antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32695-img0001.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-32695] - A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-32695-img0013.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-32695] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-32695-img0015.jpg)
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP1-32695] - IP samples: HeLa whole cell extract A. 40 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of YWHAE protein by 4 ug 14-3-3 epsilon antibody [N1C3] (NBP1-32695) 12 % SDS-PAGE The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] (NBP1-32695) diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP1-32695-img0012.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-33554-img0005.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33554-img0002.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554] - Paraffin-embedded MDA-MB231 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-33554-img0003.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP1-33554-img0004.jpg)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-99633] - Anti-14-3-3 epsilon rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-99633-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-99633] - Anti-14-3-3 epsilon rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-99633] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg SH-SY5Y Whole Cell Lysate. 4 ul anti-14-3-3 epsilon rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 epsilon rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-99633-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-99633] - Immunofluorescence staining of 14-3-3 epsilon in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99633 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-99633_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511444474.png)
![Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Western-Blot-NBP2-97726-img0003.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: U-251 MG Whole Cell LysateLane D: A375 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa")
![Immunoprecipitation: 14-3-3 epsilon Antibody [NBP2-97726] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-YWHAE rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-YWHAE rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :30 kDa](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-Immunoprecipitation-NBP2-97726-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP2-97726] - Immunofluorescence staining of 14-3-3 epsilon in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97726 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97726_rabbit-polyclonal-14-3-3-epsilon-antibody-235202511545662.png)
![Western Blot: 14-3-3 epsilon Antibody (4F8) [NBP2-59395] - Lane 1: 14-3-3 Zeta Recombinant Protein, Lane 2: 14-3-3 Beta Recombinant Protein, Lane 3: 14-3-3 Epsilon Recombinant Protein, Lane 4: 14-3-3 Eta Recombinant Protein, Lane 5: 14-3-3 Gamma Recombinant Protein, Lane 6: 14-3-3 Sigma Recombinant Protein, Lane 7: 14-3-3 Tau Recombinant Protein](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-4F8-Western-Blot-NBP2-59395-img0001.jpg "Western Blot: 14-3-3 epsilon Antibody (4F8) [NBP2-59395] - Lane 1: 14-3-3 Zeta Recombinant Protein, Lane 2: 14-3-3 Beta Recombinant Protein, Lane 3: 14-3-3 Epsilon Recombinant Protein, Lane 4: 14-3-3 Eta Recombinant Protein, Lane 5: 14-3-3 Gamma Recombinant Protein, Lane 6: 14-3-3 Sigma Recombinant Protein, Lane 7: 14-3-3 Tau Recombinant Protein")
![Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody (4F8) [NBP2-59395] - Analysis of 14-3-3 epsilon in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of 14-3-3 epsilon in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 epsilon antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-4F8-Immunocytochemistry-Immunofluorescence-NBP2-59395-img0002.jpg)
![Western Blot: 14-3-3 epsilon Antibody (4X6E1) [NBP3-16515] - Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon Rabbit mAb (NBP3-16515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Antibody-4X6E1-Western-Blot-NBP3-16515-img0001.jpg "Western Blot: 14-3-3 epsilon Antibody (4X6E1) [NBP3-16515] - Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon Rabbit mAb (NBP3-16515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - NBP3-26078 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lane D: A375 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa](http://images.novusbio.com/fullsize/nbp3-26078_mouse-14-3-3-epsilon-mab-02-azide-and-bsa-free-136202422345087.png "Western Blot: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - NBP3-26078 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: U-251 MG Whole Cell Lysate. Lane D: A375 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa")
![Immunoprecipitation: 14-3-3 epsilon Antibody (02) - Azide and BSA Free [NBP3-26078] - 14-3-3 epsilon was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. Lane C: 0.5 mg SH-SY5Y Whole Cell Lysate 4 uL NBP3-26078 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-26078, at 1:100 dilution. Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2 [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa](http://images.novusbio.com/fullsize/nbp3-26078_mouse-14-3-3-epsilon-mab-02-azide-and-bsa-free-136202422345019.png)
![Western Blot: 14-3-3 epsilon Overexpression Lysate (Adult Normal) [NBP2-04318] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 epsilon.](http://images.novusbio.com/fullsize/14-3-3-epsilon-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04318-img0001.jpg "Western Blot: 14-3-3 epsilon Overexpression Lysate (Adult Normal) [NBP2-04318] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 14-3-3 epsilon.")
