Products Related to Gene-ID: 6772

HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox"> HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-a2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_lightbox"> 11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol." class="big_thumb" />

11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT1 (Y701) at approximately 86 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT1 (Y701) at approximately 86 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF109). A specific band was detected for STAT1 p91 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF109). A specific band was detected for STAT1 p91 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for STAT1 p91 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB14901). View our recommended buffer recipes for immunoprecipitation." class="big_lightbox"> MAB14901). View our recommended buffer recipes for immunoprecipitation." class="big_thumb" />

HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

11100-1) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human Phospho-STAT1 (Y701) Monoclonal Antibody (Catalog # MAB2894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 11100-1) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human Phospho-STAT1 (Y701) Monoclonal Antibody (Catalog # MAB2894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human Phospho-STAT1 (Y701) Monoclonal Antibody (Catalog # MAB2894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="STAT1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human STAT1 Monoclonal Antibody (Catalog # MAB1490) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB0061, open histogram) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB0061, open histogram) followed by APC-conjugated Goat anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

HAF007). A specific band was detected for STAT1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for STAT1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse STAT1 Monoclonal Antibody (Catalog # MAB14091) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for STAT1 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />