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![Immunocytochemistry/Immunofluorescence: BRCA2 Antibody [NBP1-88361] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BRCA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88361-img0011.jpg "Immunocytochemistry/Immunofluorescence: BRCA2 Antibody [NBP1-88361] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Flow Cytometry: BRCA2 Antibody [NBP1-88361] - DNA damage induced by low-dose radiation and cfDNAox and cfDNAoxR fragments activates reparation of nuclear DNA. (a) (1) Flow cytometry detection of BRCA2 in irradiated (10cGy) and control cells stained with anti-BRCA2 antibodies and secondary FITC-conjugated antibodies. Gate R encircles the fraction of MSCs with elevated values of FL1- BRCA2; (2) distribution of cells with varying BRCA2 contents. Image collected and cropped by CiteAb from the following publication (hindawi.com/journals/omcl/2017/9515809/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BRCA2-Antibody-Flow-Cytometry-NBP1-88361-img0012.jpg)
![Western Blot: BRCA2 Antibody (5F6) [NBP1-41189] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA2 antibody [5F6] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp1-41189_mouse-monoclonal-brca2-antibody-5f6-western-blot-2962023104449..jpg "Western Blot: BRCA2 Antibody (5F6) [NBP1-41189] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA2 antibody [5F6] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![SDS-Page: BRCA2 Recombinant Protein [H00000675-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/BRCA2-Recombinant-Protein-SDS-Page-H00000675-Q01-img0001.jpg "SDS-Page: BRCA2 Recombinant Protein [H00000675-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunoprecipitation:BRCA2 AntibodyNBP3-30709] -Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRCA2 antibody NBP3-30709 (lot NBP3-30709-4) used for IP at 6 µg per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30709-3). For blotting immunoprecipitated BRCA2, NBP3-30709 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30709_rabbit-brca2-pab-301220241836171.jpg "Immunoprecipitation:BRCA2 AntibodyNBP3-30709] -Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRCA2 antibody NBP3-30709 (lot NBP3-30709-4) used for IP at 6 µg per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30709-3). For blotting immunoprecipitated BRCA2, NBP3-30709 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![ELISA: Human BRCA2 ELISA Kit (Chemiluminescence) [NBP2-70019] - Samples were spiked with high concentrations of Human BRCA2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70019_human-brca2-elisa-kit-chemiluminescence-132202416143023.jpg "ELISA: Human BRCA2 ELISA Kit (Chemiluminescence) [NBP2-70019] - Samples were spiked with high concentrations of Human BRCA2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human BRCA2 ELISA Kit (Chemiluminescence) [NBP2-70019] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70019_human-brca2-elisa-kit-chemiluminescence-4320249115654.jpg)
![Immunoprecipitation:BRCA2 AntibodyNBP3-28966] -Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRCA2 antibody NBP3-28966 (lot NBP3-28966-3) used for IP at 6 µg per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-28966-2). For blotting immunoprecipitated BRCA2, NBP3-28966 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-28966_rabbit-brca2-pab-301220241536291.jpg "Immunoprecipitation:BRCA2 AntibodyNBP3-28966] -Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRCA2 antibody NBP3-28966 (lot NBP3-28966-3) used for IP at 6 µg per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-28966-2). For blotting immunoprecipitated BRCA2, NBP3-28966 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: BRCA2 Antibody (BRCA2/2158) [NBP3-23660] - Formalin-fixed, paraffin-embedded human salivary gland stained with BRCA2 Antibody (BRCA2/2158). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23660_mouse-brca2-mab-brca2-2158-2412025872340.jpg "Immunohistochemistry-Paraffin: BRCA2 Antibody (BRCA2/2158) [NBP3-23660] - Formalin-fixed, paraffin-embedded human salivary gland stained with BRCA2 Antibody (BRCA2/2158). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.")
![Immunoprecipitation:BRCA2 AntibodyNBP3-29158] -Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRCA2 antibody NBP3-29158 used for IP at 6 µg/mg lysate. BRCA2 was also immunoprecipitated by rabbit anti-BRCA2., which recognizes an upstream epitope. For blotting immunoprecipitated BRCA2, was used at 1 µg/ml,. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29158_rabbit-brca2-pab-301220241822253.jpg "Immunoprecipitation:BRCA2 AntibodyNBP3-29158] -Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRCA2 antibody NBP3-29158 used for IP at 6 µg/mg lysate. BRCA2 was also immunoprecipitated by rabbit anti-BRCA2., which recognizes an upstream epitope. For blotting immunoprecipitated BRCA2, was used at 1 µg/ml,. Detection: Chemiluminescence with an exposure time of 10 seconds.")