VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Smoothened was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Smoothened Monoclonal Antibody (Catalog # MAB10276) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10276-SP | |||
![Immunohistochemistry-Paraffin: Smoothened Antibody [NLS2666] - Analysis of anti-Smoothened antibody with human kidney.](http://images.novusbio.com/fullsize/Smoothened-Antibody-Immunohistochemistry-Paraffin-NLS2666-img0003.jpg "Immunohistochemistry-Paraffin: Smoothened Antibody [NLS2666] - Analysis of anti-Smoothened antibody with human kidney.")
![Western Blot: Smoothened Antibody [NBP3-06699] - Western blot analysis of lysates from HUVEC cells, using Smoothened Antibody. The lane on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/Smoothened-Antibody-Western-Blot-NBP3-06699-img0003.jpg "Western Blot: Smoothened Antibody [NBP3-06699] - Western blot analysis of lysates from HUVEC cells, using Smoothened Antibody. The lane on the right is blocked with the synthesized peptide.")
![Immunohistochemistry-Paraffin: Smoothened Antibody [NBP3-06699] - Human Breast, Lobule: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Smoothened-Antibody-Immunohistochemistry-Paraffin-NBP3-06699-img0008.jpg)
![SDS-Page: Recombinant Human Smoothened Protein [H00006608-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006608-Q01-1.jpg "SDS-Page: Recombinant Human Smoothened Protein [H00006608-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Smoothened Overexpression Lysate (Adult Normal) [NBP2-10451] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Smoothened.](http://images.novusbio.com/fullsize/Smoothened-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10451-img0001.jpg "Western Blot: Smoothened Overexpression Lysate (Adult Normal) [NBP2-10451] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Smoothened.")
![Western Blot: Rabbit Smoothened pAb [NBP3-30939] - Analysis using Smoothened pAb on human brain lysate. Lysate loaded at 15µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP, used at 1:150K dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-30939_rabbit-smoothened-pab-western-blot-16122024141619..jpg "Western Blot: Rabbit Smoothened pAb [NBP3-30939] - Analysis using Smoothened pAb on human brain lysate. Lysate loaded at 15µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP, used at 1:150K dilution.")
![Western Blot: Smoothened Antibody [NBP3-30919] - Western blot using SMO antibody on human brain lysate. Lysate loaded at 15ug/lane. Antibody used at 10 ug/ml (1) and 5 ug/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP , used at 1:150K dilution.](http://images.novusbio.com/fullsize/nbp3-30919_rabbit-smoothened-pab-811202420592185.jpg "Western Blot: Smoothened Antibody [NBP3-30919] - Western blot using SMO antibody on human brain lysate. Lysate loaded at 15ug/lane. Antibody used at 10 ug/ml (1) and 5 ug/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP , used at 1:150K dilution.")
![Western Blot: Smoothened Antibody (1D9) [H00006608-M11] - Analysis of SMO expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/Smoothened-Antibody-1D9-Western-Blot-H00006608-M11-img0002.jpg "Western Blot: Smoothened Antibody (1D9) [H00006608-M11] - Analysis of SMO expression in A-431 (Cat # L015V1).")
![Sandwich ELISA: Smoothened Antibody (3E5) [H00006608-M31] - Detection limit for recombinant GST tagged SMO is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smoothened-Antibody-3E5-Sandwich-ELISA-H00006608-M31-img0001.jpg "Sandwich ELISA: Smoothened Antibody (3E5) [H00006608-M31] - Detection limit for recombinant GST tagged SMO is 0.3 ng/ml as a capture antibody.")
![Sandwich ELISA: Smoothened Antibody (3D10) [H00006608-M14] - Detection limit for recombinant GST tagged SMO is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Smoothened-Antibody-3D10-Sandwich-ELISA-H00006608-M14-img0001.jpg "Sandwich ELISA: Smoothened Antibody (3D10) [H00006608-M14] - Detection limit for recombinant GST tagged SMO is 0.1 ng/ml as a capture antibody.")
