| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1568-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1568-HP-050/CF | |||
8908-SH) in C3H10T1/2 mouse embryonic fibroblasts with an ED50 = 0.1-0.6 µg/mL (orange line)." alt="Recombinant Human Sonic Hedgehog/Shh (Catalog # 8908-SH) induces alkaline phosphatase production in C3H10T1/2 cells (green line). Recombinant Human Hip (Catalog # 9280-HP) inhibits a constant dose of 20 ng/mL of Recombinant Human Sonic Hedgehog/Shh Protein, High Activity (Catalog # 8908-SH) in C3H10T1/2 mouse embryonic fibroblasts with an ED50 = 0.1-0.6 µg/mL (orange line)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1568-SP | |||
![Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-2120249362721.jpg "Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1969] (NBP3-25506) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-212024937498.jpg)
![Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-212024943556.jpg)
![Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-212024941444.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1969] (NBP3-25506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-2120249435526.jpg)
![Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HHIP stained by HHIP antibody [HL1737] (NBP3-25505) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25505_rabbit-hip-mab-hl1737-azide-and-bsa-free-2120249351335.jpg "Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HHIP stained by HHIP antibody [HL1737] (NBP3-25505) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1737] (NBP3-25505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25505_rabbit-hip-mab-hl1737-azide-and-bsa-free-2120249371557.jpg)
![Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HHIP stained by HHIP antibody [HL1737] (NBP3-25505) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25505_rabbit-hip-mab-hl1737-azide-and-bsa-free-2120249355559.jpg)
![Western Blot: Hip Antibody [H00064399-D01P] - Analysis of HHIP expression in transfected 293T cell line by HHIP polyclonal antibody.Lane 1: HHIP transfected lysate(78.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Hip-Antibody-Western-Blot-H00064399-D01P-img0001.jpg "Western Blot: Hip Antibody [H00064399-D01P] - Analysis of HHIP expression in transfected 293T cell line by HHIP polyclonal antibody.Lane 1: HHIP transfected lysate(78.90 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Hip Antibody [NBP2-16821] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [C1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hip-Antibody-Western-Blot-NBP2-16821-img0003.jpg "Western Blot: Hip Antibody [NBP2-16821] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [C1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Hip Antibody [NBP2-16821] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hip-Antibody-Western-Blot-NBP2-16821-img0002.jpg)
![Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11. Analysis of HHIP expression in MCF-7.](http://images.novusbio.com/fullsize/Hip-Antibody-5D11-Western-Blot-H00064399-M01-img0018.jpg "Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11. Analysis of HHIP expression in MCF-7.")
![Immunohistochemistry-Paraffin: Hip Antibody (5D11) [H00064399-M01] - Analysis of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Hip-Antibody-5D11-Immunohistochemistry-Paraffin-H00064399-M01-img0014.jpg)
![Western Blot: Hip Antibody (5D11) [H00064399-M01] - HHIP monoclonal antibody (M01), clone 5D11 Analysis of HHIP expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Hip-Antibody-5D11-Western-Blot-H00064399-M01-img0017.jpg)
![Western Blot: Hip Antibody (5D11) [H00064399-M01] - Analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody (M01), clone 5D11.Lane 1: HHIP transfected lysate(78.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Hip-Antibody-5D11-Western-Blot-H00064399-M01-img0019.jpg)
![Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01] - HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HHIP MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Hip-Antibody-5D11-Immunoprecipitation-H00064399-M01-img0009.jpg)
![Immunoprecipitation: Hip Antibody (5D11) [H00064399-M01] - Analysis of HHIP transfected lysate using anti-HHIP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HHIP MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Hip-Antibody-5D11-Immunoprecipitation-H00064399-M01-img0015.jpg)
![ELISA: Hip Antibody (5D11) [H00064399-M01] - Detection limit for recombinant GST tagged HHIP is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hip-Antibody-5D11-ELISA-H00064399-M01-img0020.jpg)
