| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38632-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1827-SF-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81866-25ul | |||
HAF019). A specific band was detected for sFRP-4 at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of OVCAR-3 human ovarian carcinoma cell line, human ovarian cancer tissue, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human sFRP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for sFRP-4 at approximately 53-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1827-SP | |||
1324-WN)." alt="Recombinant Mouse sFRP-4 (Catalog # 9806-SF) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.2-1.2 µg/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN)." class="big_thumb" />
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium, kidney, liver and placenta using Anti-SFRP4 antibody (A) shows similar protein distribution across tissues to independent antibody (B).](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0017.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium, kidney, liver and placenta using Anti-SFRP4 antibody (A) shows similar protein distribution across tissues to independent antibody (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: sFRP-4 Antibody [NBP2-38632] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Western-Blot-NBP2-38632-img0002.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium shows moderate secreted positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0012.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0013.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0014.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP2-38632-img0015.jpg)
![Western Blot: sFRP-4 Antibody [NBP1-81866] - Analysis in control (vector only transfected HEK293T lysate) and sFRP4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Western-Blot-NBP1-81866-img0009.jpg "Western Blot: sFRP-4 Antibody [NBP1-81866] - Analysis in control (vector only transfected HEK293T lysate) and sFRP4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP1-81866-img0023.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866] - Staining of human endometrium shows moderate secreted positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP1-81866-img0020.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP1-81866-img0021.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-81866] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP1-81866-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunofluorescence analysis of NIH-3T3 cells using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-7Z6Q1-Immunocytochemistry-Immunofluorescence-NBP3-16352-img0002.jpg "Immunocytochemistry/Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunofluorescence analysis of NIH-3T3 cells using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Immunohistochemistry of paraffin-embedded human colon using sFRP-4 Rabbit mAb (NBP3-16352) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-7Z6Q1-Immunohistochemistry-Paraffin-NBP3-16352-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Confocal imaging of U-2 OS cells using sFRP-4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-5220251215490.jpg)
![Immunocytochemistry/ Immunofluorescence: sFRP-4 Antibody (7Z6Q1) [NBP3-16352] - Confocal imaging of NIH/3T3 cells using sFRP-4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-5220251242529.jpg)
![Western Blot: sFRP-4 Antibody (7Z6Q1) [sFRP-4] - Western blot analysis of various lysates using sFRP-4 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16352_rabbit-sfrp-4-mab-7z6q1-52202517192946.jpg)
![Western Blot: sFRP-4 Antibody [NBP1-36985] - Western blot analysis of SFRP4 in Human Uterus lysate (35ug protein in RIPA buffer) using NBP1-36985 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Western-Blot-NBP1-36985-img0006.jpg "Western Blot: sFRP-4 Antibody [NBP1-36985] - Western blot analysis of SFRP4 in Human Uterus lysate (35ug protein in RIPA buffer) using NBP1-36985 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-36985] - Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Immunohistochemistry-Paraffin-NBP1-36985-img0007.jpg)
![Immunohistochemistry-Paraffin: sFRP-4 Antibody (5#) [NBP3-41642] - DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 10ug/ml NBP3-41642; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41642_mouse-sfrp-4-mab-5-6122024212721.jpg "Immunohistochemistry-Paraffin: sFRP-4 Antibody (5#) [NBP3-41642] - DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 10ug/ml NBP3-41642; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: sFRP-4 Antibody (5#) [NBP3-41642] - Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Porcine Heart lysate; Lane4: SKOV3 cell lysate Primary Ab: 2ug/ml NBP3-41642; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41642_mouse-sfrp-4-mab-5-61220242195744.jpg)
![Western Blot: sFRP-4 Antibody (5#) [NBP3-41642] - Sample: Recombinant sFRP-4, Human.](http://images.novusbio.com/fullsize/nbp3-41642_mouse-sfrp-4-mab-5-6122024212769.jpg)
![Western Blot: sFRP-4 Antibody (S01-2C2) [NBP3-14974] - Western blot detection of sFRP-4 in K562, 3T3, Hela cell lysates using sFRP-4 Rabbit mAb (1:1000 diluted). Predicted band size: 40kDa. Observed band size: 53-55kDa.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-S01-2C2-Western-Blot-NBP3-14974-img0001.jpg "Western Blot: sFRP-4 Antibody (S01-2C2) [NBP3-14974] - Western blot detection of sFRP-4 in K562, 3T3, Hela cell lysates using sFRP-4 Rabbit mAb (1:1000 diluted). Predicted band size: 40kDa. Observed band size: 53-55kDa.")
![Western Blot: sFRP-4 Antibody [NBP2-20330] - Sample (30 ug of whole cell lysate) A: A549 B: Hep G2 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/sFRP-4-Antibody-Western-Blot-NBP2-20330-img0002.jpg "Western Blot: sFRP-4 Antibody [NBP2-20330] - Sample (30 ug of whole cell lysate) A: A549 B: Hep G2 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: SFRP4 Overexpression Lysate (Adult Normal) [NBL1-15885] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SFRP4.](http://images.novusbio.com/fullsize/SFRP4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15885-img0002.jpg "Western Blot: SFRP4 Overexpression Lysate (Adult Normal) [NBL1-15885] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SFRP4.")
