| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24911 | |||
| BSA Free | NB100-56559SS | |||
CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hair follicle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="S100A7 was detected in immersion fixed paraffin-embedded sections of human skin (hair follicle) using Mouse Anti-Human S100A7 Monoclonal Antibody (Catalog # MAB4475) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to hair follicle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4475-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4475-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99720-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87205-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89814-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89813-50ul | |||
![Western Blot: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Psoriasin in A) MCF10A and B) MCF7 cell lysate using psoriasin antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Western-Blot-NB100-56559-img0014.jpg "Western Blot: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Psoriasin in A) MCF10A and B) MCF7 cell lysate using psoriasin antibody at 1 ug/mL.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - S100A7/psoriasin detection was performed on (A) untreated and (B) LPS-treated (100 ng/mL, 21 hrs) HOPE-fixed NCI-H727 cells using the monoclonal Psoriasin/HID5/S100A7 antibody (NB100-56559). (C) Positive control is shown by the expression of the nuclear Ki67 antigen with the MIB-1 (2 ug/mL) antibody. (D) Negative control was included omitting the primary antibody.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Immunocytochemistry-Immunofluorescence-NB100-56559-img0005.jpg)
![Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.. Using the PE format of this antibody.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Flow-Intracellular-NB100-56559-img0020.jpg)
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Staining of human skin. FFPE tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/mL.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Immunohistochemistry-Paraffin-NB100-56559-img0006.jpg)
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Tissue section of human esophageal squamous cell carcinoma (SCC) using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone 47C1068). Diffused to granular cytoplasmic immunostaining was observed all over the tissue section with highest expression levels in the areas with cancer cells especially in the developing squamous keratin pearls.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Immunohistochemistry-Paraffin-NB100-56559-img0008.jpg)
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis of FFPE tissue section of human lymph node cancer using 5 ug/mL concentration of Psoriasin/S100A7 antibody (clone 47C1068). Very strong cytoplasmic immunopositivity of Psoriasin/S100A7 protein was observed in the cancer cells.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Immunohistochemistry-Paraffin-NB100-56559-img0009.jpg)
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Human breast tissue stained with psoriasin antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Antigen retrieval with citrate buffer, pH 6.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Immunohistochemistry-Paraffin-NB100-56559-img0011.jpg)
![Flow (Intracellular): S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - An intracellular stain was performed on Jurkat cells with S100A7/Psoriasin antibody (47C1068) NBP2-24911PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Flow-Intracellular-NB100-56559-img0017.jpg)
![Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Image shows a specific band for S100A7/Psoriasin in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Simple-Western-NB100-56559-img0010.jpg)
![Simple Western: S100A7/Psoriasin Antibody (47C1068) [NB100-56559] - Analysis using Azide Free version of NB100-56559. Image shows a specific band for Psoriasin/S100A7 in 0.05 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-47C1068-Simple-Western-NB100-56559-img0015.jpg)
![Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 activated p53 related anti-proliferative pathways. (A) Log2 fold change of mRNA expression of p53 pathway factors after S100A7 overexpression. (B) WB of p53 & S100A7 in MCF7-p53-CKD transfected with S100A7 or vector control. p53 shRNA transcription & p53 knockdown in MCF7-p53-CKD was induced by Doxycyclin (Dox). (C) S100A7 activated p53, ATR, chk-1, chk-2 in MCF7 cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25622979), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56559_mouse-monoclonal-s100a7-psoriasin-antibody-47c1068-31020241529196.jpg)
![Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 induces EMT in cervical cancer cellsWestern Blot showed the protein level of epithelial marker E-cadherin & mesenchymal markers (N-Cadherin, Vimentin, Fibronectin) & EMT transcription factors Snail & Slug after overexpression of S100A7 in C33A cells A. & SiHa cells B. beta -actin is used as a loading control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15329), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56559_mouse-monoclonal-s100a7-psoriasin-antibody-47c1068-310202415334934.jpg)
![Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 interacts with RAGE, stimulates ERK pathway & RAGE mediates S100A7-induced migration & invasion in C33A & SiHa cellsA. HEK293 cells were transiently co-expressed with pcDNA3-Flag & pcDNA3-Flag-RAGE with pcDNA3-myc-S100A7. Cell extracts were immunoprecipitated separately with specific antibody against Flag, & the associated Flag-RAGE & myc-S100A7 were examined by Western blotting using tagged antibodies, respectively. B. S100A7-expressing cells were found to have higher levels of phospho-ERK (without any change in total ERK levels). In comparison, the levels of phospho-Akt & phospho-NF-kappa B had no obvious change. C. qRT-PCR verification of RAGE knockdown by two independent siRNAs in C33A & SiHa cells. D&E. RAGE knockdown suppressed migration & invasion in S100A7-overexpressed C33A & SiHa cells & their corresponding control cells. The stained cells were manually counted from 4 randomly selected fields & normalized with cell proliferation [2-sided t test; * P < 0.05; **P < 0.01]. Representative image & quantitative results of cell migration & invasion were shown (D. C33A cells; E. SiHa cells; ×10, bars: 100μm). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15329), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56559_mouse-monoclonal-s100a7-psoriasin-antibody-47c1068-31020241555244.jpg)
![Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 is secreted & acts as a chemotactic factor of cell migration & invasionA. Cells were cultured in serum-free medium for 2 days, then the medium was collected, fractioned, followed by Western Blot analysis. B&C. Transwell migration & Matrigel invasion assays were performed in C33A & SiHa cells. Cell suspension was placed into the upper chamber in 0.1 ml of DMEM serum-free medium, & conditioned medium from S100A7-overexpressed C33A & SiHa cells & their corresponding control cells was placed in the lower chamber as a chemoattractant. The stained cells were manually counted from 4 randomly selected fields & normalized with cell proliferation [2-sided t test; *P < 0.05; ***P < 0.001]. Representative image & quantitative results of cell migration & invasion were shown (B. C33A cells; C. SiHa cells; ×10, bars:100μm). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15329), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56559_mouse-monoclonal-s100a7-psoriasin-antibody-47c1068-310202415535176.jpg)
![Western Blot: S100A7/Psoriasin Antibody (47C1068) - BSA Free [NB100-56559] - S100A7 promotes cervical cancer cell migration & invasionA&B. Establishment of stable cell lines of ectopic expression of S100A7. C33A & SiHa cells were infected with pLVX-Con & pLVX-S100A7 lentivirus, stable cells were established by Geneticin (G418) selection for about 2 weeks. Cells were harvested, S100A7 expression was detected by qRT-PCR (A: [mean (n=2) ± SD; 2-sided t test; ** P<0.01, normalized to beta -actin]) & Western Blot. C&D. Transwell migration & Matrigel invasion assays were performed in S100A7-overexpressed cells & their corresponding control cells. The stained cells were manually counted from 4 randomly selected fields & normalized with cell proliferation [2-sided t test; **P < 0.01]. Representative image & quantitative results of cell migration & invasion were shown (C. C33A cells; D. SiHa cells; ×10, bars:100μm). E. The effect of S100A7 on cell migration was examined in wound-healing assay. Confluent cells were scratched & photographed at time 0h & 48h (Left panel. C33A cells; Right panel. SiHa cells. ×10, bars:100μm). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.15329), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56559_mouse-monoclonal-s100a7-psoriasin-antibody-47c1068-310202415541988.jpg)
![Western Blot: S100A7/Psoriasin Antibody [NBP2-99720] - Anti-S100A7/Psoriasin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 15 kDa](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-Western-Blot-NBP2-99720-img0004.jpg "Western Blot: S100A7/Psoriasin Antibody [NBP2-99720] - Anti-S100A7/Psoriasin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 15 kDa")
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody [NBP2-99720] - Immunochemical staining of human S100A7/Psoriasin in human tonsil with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to tonsil crypt and epithelium. The left panel: tissue incubated with pr](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-Immunohistochemistry-Paraffin-NBP2-99720-img0003.jpg)
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody [NBP2-99720] - Immunochemical staining of human S100A7/Psoriasin in human skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to sweat gland. The left panel: tissue incubated with primary antibody; Th](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-Immunohistochemistry-Paraffin-NBP2-99720-img0002.jpg)
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody [NBP1-87205] - Staining of human skin shows strong nuclear and cytoplasmic positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-Immunohistochemistry-Paraffin-NBP1-87205-img0001.jpg)
![Immunohistochemistry: S100A7/Psoriasin Antibody [NBP1-87205] - Zn-binding protein immunohistochemical staining in OPSCC. Examples from nanozoomer digitized scans (20X magnification) of low, moderate & high cytoplasmic staining of OPSCC tumor tissue for the Zn-binding proteins AZGP1, Lipocalin-1, serum Albumin, S100A7, S100A8 & S100A9. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31740720), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87205_rabbit-polyclonal-s100a7-psoriasin-antibody-31020241535623.jpg)
![ELISA: Human S100A7/Psoriasin ELISA Kit (Colorimetric) [NBP3-40416] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40416_human-s100a7-psoriasin-elisa-kit-colorimetric-2110202415302938.png "ELISA: Human S100A7/Psoriasin ELISA Kit (Colorimetric) [NBP3-40416] - Standard Curve Reference")
![Western Blot: S100A7/Psoriasin Antibody (1C5-C6) [H00006278-M01] - Analysis of S100A7 expression in transfected 293T cell line by S100A7 monoclonal antibody (M01), clone 1C5-C6.Lane 1: S100A7 transfected lysate(11.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-1C5-C6-Western-Blot-H00006278-M01-img0006.jpg "Western Blot: S100A7/Psoriasin Antibody (1C5-C6) [H00006278-M01] - Analysis of S100A7 expression in transfected 293T cell line by S100A7 monoclonal antibody (M01), clone 1C5-C6.Lane 1: S100A7 transfected lysate(11.5 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: S100A7/Psoriasin Antibody (1C5-C6) [H00006278-M01] - Analysis of monoclonal antibody to S100A7 on HeLa cell . Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-1C5-C6-Immunocytochemistry-Immunofluorescence-H00006278-M01-img0004.jpg)
![ELISA: S100A7/Psoriasin Antibody (1C5-C6) [H00006278-M01] - Detection limit for recombinant GST tagged S100A7 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-1C5-C6-Sandwich-ELISA-H00006278-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (129) [NBP2-89814] - Staining of human S100A7 in human skin (from donor 1) with rabbit monoclonal antibody (1:200). The image showing positive staining of epithelium. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-129-Immunohistochemistry-Paraffin-NBP2-89814-img0004.jpg "Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (129) [NBP2-89814] - Staining of human S100A7 in human skin (from donor 1) with rabbit monoclonal antibody (1:200). The image showing positive staining of epithelium. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).")
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (129) [NBP2-89814] - Staining of human S100A7 in human tonsil with rabbit monoclonal antibody (1:500). The image showing positive staining in crypt.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-129-Immunohistochemistry-Paraffin-NBP2-89814-img0001.jpg)
![Immunohistochemistry-Paraffin: S100A7/Psoriasin Antibody (129) [NBP2-89814] - Staining of human S100A7 in human skin (from donor 2) with rabbit monoclonal antibody (1:500).](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-129-Immunohistochemistry-Paraffin-NBP2-89814-img0003.jpg)
![ELISA: Human S100A7/Psoriasin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40415] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40415_human-s100a7-psoriasin-ready-to-use-elisa-kit-color-2110202415305251.png "ELISA: Human S100A7/Psoriasin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40415] - Standard Curve Reference")
![Flow Cytometry: S100A7/Psoriasin Antibody (128) [NBP2-89813] - Analysis of Human S100A7 expression on HeLa cells. The cells were treated, stained with purified anti-Human S100A7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/S100A7-Psoriasin-Antibody-128-Flow-Cytometry-NBP2-89813-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: S100A7/Psoriasin Antibody (128) [NBP2-89813] - Analysis of Human S100A7 expression on HeLa cells. The cells were treated, stained with purified anti-Human S100A7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: S100A7/Psoriasin Antibody (128) [NBP2-89813] - Analysis of Human S100A7 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89813 antibody (1:60). Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody. Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89813_rabbit-monoclonal-s100a7-psoriasin-antibody-128-235202512205547.png)
![SDS-Page: Recombinant Human S100A7/Psoriasin Protein [NBP1-51061] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-S100A7-Psoriasin-Protein-SDS-Page-NBP1-51061-img0001.jpg "SDS-Page: Recombinant Human S100A7/Psoriasin Protein [NBP1-51061] - 15 % SDS-PAGE (3ug)")
