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![Western Blot: GRASP1 Antibody (1E3) [NBP2-02277] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRASP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRASP1.](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Western-Blot-NBP2-02277-img0002.jpg "Western Blot: GRASP1 Antibody (1E3) [NBP2-02277] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRASP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRASP1.")
![Immunocytochemistry/Immunofluorescence: GRASP1 Antibody (1E3) [NBP2-02277] Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRASP1.](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Immunocytochemistry-Immunofluorescence-NBP2-02277-img0009.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody (1E3) [NBP2-02277] - Staining of paraffin-embedded prostate tissue using anti-GRASP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Immunohistochemistry-Paraffin-NBP2-02277-img0008.jpg)
![Flow Cytometry: GRASP1 Antibody (1E3) [NBP2-02277] - Analysis of Hela cells, using anti-GRASP1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Flow-Cytometry-NBP2-02277-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP1 Antibody (1E3) [NBP2-02277] - Immunofluorescent staining of HepG2 cells using anti-GRASP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Immunocytochemistry-Immunofluorescence-NBP2-02277-img0003.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody (1E3) [NBP2-02277] - Staining of paraffin-embedded breast tissue using anti-GRASP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Immunohistochemistry-Paraffin-NBP2-02277-img0004.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody (1E3) [NBP2-02277] - Staining of paraffin-embedded endometrium tissue using anti-GRASP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Immunohistochemistry-Paraffin-NBP2-02277-img0005.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody (1E3) [NBP2-02277] - Staining of paraffin-embedded Kidney tissue using anti-GRASP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Immunohistochemistry-Paraffin-NBP2-02277-img0006.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody (1E3) [NBP2-02277] - Staining of paraffin-embedded Ovary tissue using anti-GRASP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRASP1-Antibody-1E3-Immunohistochemistry-Paraffin-NBP2-02277-img0007.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85103] - Staining in human cerebral cortex and skeletal muscle tissues using anti-GRIPAP1 antibody. Corresponding GRIPAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Immunohistochemistry-Paraffin-NBP1-85103-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85103] - Staining in human cerebral cortex and skeletal muscle tissues using anti-GRIPAP1 antibody. Corresponding GRIPAP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GRASP1 Antibody [NBP1-85103] - Analysis in control (vector only transfected HEK293T lysate) and GRIPAP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/GRASP1-Antibody-Western-Blot-NBP1-85103-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GRASP1 Antibody [NBP1-85103] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85103-img0005.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85103] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Immunohistochemistry-Paraffin-NBP1-85103-img0007.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85103] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Immunohistochemistry-Paraffin-NBP1-85103-img0008.jpg)
![Immunohistochemistry-Paraffin: GRASP1 Antibody [NB100-1432] - staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Immunohistochemistry-Paraffin-NB100-1432-img0002.jpg "Immunohistochemistry-Paraffin: GRASP1 Antibody [NB100-1432] - staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: GRASP1 Antibody [NB100-1432] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Immunohistochemistry-Paraffin-NB100-1432-img0001.jpg)
![Western Blot: GRASP1 Antibody [NBP2-16739] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Western-Blot-NBP2-16739-img0002.jpg "Western Blot: GRASP1 Antibody [NBP2-16739] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: GRASP1 Antibody [NBP2-16739] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Western-Blot-NBP2-16739-img0001.jpg)
![Immunoprecipitation:GRASP1 AntibodyNBP3-30494] -Detection of human GRIPAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRIPAP1 antibody NBP3-30494 (lot NBP3-30494-1) used for IP at 6 µg per reaction. GRIPAP1 was also immunoprecipitated by rabbit anti-GRIPAP1.. For blotting immunoprecipitated GRIPAP1, NBP3-30494 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30494_rabbit-grasp1-pab-301220241531190.jpg "Immunoprecipitation:GRASP1 AntibodyNBP3-30494] -Detection of human GRIPAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRIPAP1 antibody NBP3-30494 (lot NBP3-30494-1) used for IP at 6 µg per reaction. GRIPAP1 was also immunoprecipitated by rabbit anti-GRIPAP1.. For blotting immunoprecipitated GRIPAP1, NBP3-30494 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: General Receptor for phosphoinositides 1 Antibody [NB300-310] - Rat cerebellar lysate showing the specific immunolabeling of the ~95k GRASP protein, using NB300-310.](http://images.novusbio.com/fullsize/General-Receptor-for-phosphoinositides-1-Antibody-Western-Blot-NB300-310-img0001.jpg "Western Blot: General Receptor for phosphoinositides 1 Antibody [NB300-310] - Rat cerebellar lysate showing the specific immunolabeling of the ~95k GRASP protein, using NB300-310.")
![Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85102] - Staining of human gall bladder shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Immunohistochemistry-Paraffin-NBP1-85102-img0002.jpg "Immunohistochemistry-Paraffin: GRASP1 Antibody [NBP1-85102] - Staining of human gall bladder shows cytoplasmic positivity in glandular cells.")
![Western Blot: GRASP1 Antibody [NBP1-31077] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Western-Blot-NBP1-31077-img0003.jpg "Western Blot: GRASP1 Antibody [NBP1-31077] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE, antibody diluted at 1:500.")
![Western Blot: GRASP1 Antibody [NBP2-16738] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/GRASP1-Antibody-Western-Blot-NBP2-16738-img0001.jpg "Western Blot: GRASP1 Antibody [NBP2-16738] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: GRASP1 Overexpression Lysate (Adult Normal) [NBL1-11341] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRASP1.](http://images.novusbio.com/fullsize/GRASP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11341-img0002.jpg "Western Blot: GRASP1 Overexpression Lysate (Adult Normal) [NBL1-11341] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRASP1.")
![Immunoprecipitation:GRASP1 AntibodyNBP3-30165] -Detection of human GRIPAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRIPAP1 antibody NBP3-30165 (lot NBP3-30165-1) used for IP at 6 µg per reaction. GRIPAP1 was also immunoprecipitated by rabbit anti-GRIPAP1.. For blotting immunoprecipitated GRIPAP1, NBP3-30165 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30165_rabbit-grasp1-pab-3012202413324230.jpg "Immunoprecipitation:GRASP1 AntibodyNBP3-30165] -Detection of human GRIPAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GRIPAP1 antibody NBP3-30165 (lot NBP3-30165-1) used for IP at 6 µg per reaction. GRIPAP1 was also immunoprecipitated by rabbit anti-GRIPAP1.. For blotting immunoprecipitated GRIPAP1, NBP3-30165 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")