HTRA1/PRSS11: Novus Biologicals

MAB2916

HTRA1/PRSS11 Antibody (275615) [Unconjugated]

100 μg

MAB2916

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB2916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA1/PRSS11 at approximately 51 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

$HtrA1 Protein Expression in hBC cell lines.Cells (as indicated) were extracted into nuclear (N) and cytoplasmic (C) fractions as described (Materials & Methods), and proteins were analyzed by Immunoblot analyses using the polyclonal antibody against human HtrA1. beta -actin was used as a loading control, and DEK was used to assess the nuclear/cytoplasmic fractionation (DEK is exclusively nuclear). The larger Mr bands seen in the MCF12A and MCF10A/Flp cell lines are consistent with dimers, trimers, etc., although this was not confirmed. MCF10A/Flp is the parental Flp-in cell line which was used to produce the various MCF10A/siRNA or Htra1 cell lines. Results are from a representative experiment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22761798), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$Characterization of MCF10A cell lines carrying HtrA1 siRNA and overexpression vectors.A random antisense oligonucleotide library was used to identify optimally accessible sites in HtrA1 mRNA. SiRNAs (short hairpin RNAs) were designed to target these sites, and stably transfected cell lines were developed from MCF10A cells (4 independent cell lines, designated MCF10A/siRNA1-4). A control cell line expressing an irrelevant siRNA (designated MCF10A/HPVsh) was also developed. In parallel, we also developed a cell line over-expressing HtrA1 (designated MCF10A/HtrA1). (A). Target sites empirically identified in HtrA1 mRNA. Identified domains within HtrA1 include: SS, signal sequence; IGFBP, IGF binding site; KI, Kazal Type I protease inhibitor domain; Trypsin-like protease domain; PDZ, PDZ binding domain. (B) Immunoblot analysis for HtrA1 protein. Cytoplasmic and nuclear protein fractions were prepared from the developed cell lines (as indicated), and were probed with a polyclonal antibody preparation directed against a region in the trypsin-like protease domain. As is evident, the reductions in HtrA1 protein levels were >90% compared with the various MCF10A and MCF10A/HPVsh cells. Right panel shows HtrA1 in concentrated culture medium. (C) Cells were plated and growth was measured over a 6 day period. Both of the MCF10A cell lines tested (MCF10A/siRNA1 and siRNA4) grew significantly faster than the control cells (p<0.01 at days 4 and 6). Over-expression of HtrA1 in the MCF10A/HtrA1 cells had no effect on cell growth rate. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22761798), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2916-SP

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NBP2-23869

HTRA1/PRSS11 Antibody - BSA Free

(2 Publications)

0.1 ml

NBP2-23869

Reactivity:

Hu, Mu, Rt, Bv, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6518A

Alt Catalog# IMG-6518A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-23869SS

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2916-SE

Recombinant Human HTRA1 Protein, CF

(5 Publications)

20 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB29161

HTRA1/PRSS11 Antibody (275603) [Unconjugated]

100 μg

MAB29161

HTRA1/PRSS11 was detected in immersion fixed paraffin-embedded sections of human fibrolamellar hepatocellular carcinoma and IBS intestine (positive staining; upper panels) and human cervical squamous cell carcinoma and smooth muscle (negative staining; lower panels) using Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB29161) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane (luminal surface of intestinal gland). View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB29161-SP

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1670-SE

Recombinant E. coli HTRA/Protease Do Protein, CF

20 μg

Reactivity:

Ec

Species Glossary

Reactivity: Ec

Applications:

Applications:

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NBP2-23869PEP

HTRA1/PRSS11 Antibody Blocking Peptide

0.1 mg

Applications:

Applications:

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NBP1-81654

HTRA1/PRSS11 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-81654

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-81654-25ul

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NBP2-76585

Human HTRA1/PRSS11 ELISA Kit (Colorimetric)

(1 Publication)

1 Kit

NBP2-76585

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.313 - 20ng/mL

Sensitivity:

0.188ng/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP3-27848

HTRA1/PRSS11 Antibody (SR4329) - BSA Free

100 ul

NBP3-27848

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-27848-50ul

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NBL1-11785

HTRA1/PRSS11 Overexpression Lysate

0.1 mg

NBL1-11785

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-28037

HTRA1/PRSS11 Antibody (FHTR2163) - Low Endotoxin, Azide and BSA Free

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28037AF350
Alexa Fluor 405	NBP3-28037AF405
Alexa Fluor 488	NBP3-28037AF488
Alexa Fluor 532	NBP3-28037AF532
Alexa Fluor 594	NBP3-28037AF594
Alexa Fluor 647	NBP3-28037AF647
Alexa Fluor 700	NBP3-28037AF700
Alexa Fluor 750	NBP3-28037AF750
Allophycocyanin	NBP3-28037APC
Biotin	NBP3-28037B
CoraFluor 1	NBP3-28037CL1
DyLight 350	NBP3-28037UV
DyLight 405	NBP3-28037V
DyLight 488	NBP3-28037G
DyLight 550	NBP3-28037R
DyLight 594	NBP3-28037DL594
DyLight 650	NBP3-28037C
DyLight 680	NBP3-28037FR
DyLight 755	NBP3-28037IR
FITC	NBP3-28037F
HRP	NBP3-28037H
Janelia Fluor 525	NBP3-28037JF525
Janelia Fluor 549	NBP3-28037JF549
Janelia Fluor 646	NBP3-28037JF646
Janelia Fluor 669	NBP3-28037JF669
PE	NBP3-28037PE
PerCP	NBP3-28037PCP
mFluor Violet 450 SE	NBP3-28037MFV450
mFluor Violet 500 SE	NBP3-28037MFV500
mFluor Violet 610 SE	NBP3-28037MFV610

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NBP1-81654PEP

HTRA1/PRSS11 Recombinant Protein Antigen

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 5654

HTRA1/PRSS11 Antibody (275615) [Unconjugated]

HTRA1/PRSS11 Antibody - BSA Free

Recombinant Human HTRA1 Protein, CF

HTRA1/PRSS11 Antibody (275603) [Unconjugated]

Recombinant E. coli HTRA/Protease Do Protein, CF

HTRA1/PRSS11 Antibody Blocking Peptide

HTRA1/PRSS11 Antibody - BSA Free

Human HTRA1/PRSS11 ELISA Kit (Colorimetric)

HTRA1/PRSS11 Antibody (SR4329) - BSA Free

HTRA1/PRSS11 Overexpression Lysate

HTRA1/PRSS11 Antibody (FHTR2163) - Low Endotoxin, Azide and BSA Free

HTRA1/PRSS11 Recombinant Protein Antigen