| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56446SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98810-50ul | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="p38 beta /MAPK11 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human p38 beta /MAPK11 Monoclonal Antibody (Catalog # MAB3274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3274-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48826-25ul | |||
![Western Blot: p38 beta/MAPK11 Antibody [NB100-56446] - Analysis of p38 beta/MAPK11 in 15 ug of A) human brain and B) mouse brain cell lysate using this antibody.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-Western-Blot-NB100-56446-img0003.jpg "Western Blot: p38 beta/MAPK11 Antibody [NB100-56446] - Analysis of p38 beta/MAPK11 in 15 ug of A) human brain and B) mouse brain cell lysate using this antibody.")
![Western Blot: p38 beta/MAPK11 Antibody [NBP2-98810] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:36 kDa](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-Western-Blot-NBP2-98810-img0004.jpg "Western Blot: p38 beta/MAPK11 Antibody [NBP2-98810] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:36 kDa")
![Immunohistochemistry-Paraffin: p38 beta/MAPK11 Antibody [NBP2-98810] - Immunochemical staining of human p38 beta/MAPK11 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-Immunohistochemistry-Paraffin-NBP2-98810-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: p38 beta/MAPK11 Antibody [NBP2-98810] - Staining of MAPK11 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-98810 antibody (dilution ratio: 1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98810_rabbit-polyclonal-p38-beta-mapk11-antibody-235202521451726.png)
![Immunohistochemistry-Paraffin: p38 beta/p38 beta/MAPK11 Antibody [NBP2-98810] - Immunochemical staining of human p38 beta/MAPK11 in human liver with NBP2-98810 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-98810_rabbit-polyclonal-p38-beta-mapk11-antibody-23520251129357.png)
![Western Blot: p38 beta/MAPK11 Antibody (E13-Q) [NBP3-08206] - Western blot analysis of p38 beta/MAPK11. Lane A - human recombinant p38-alpha (260-360a.a., N-terminal GST tag; H00001432-Q01), lane B - human recombinant p38 beta/MAPK11 (with C-terminal DDK tag, NBL1-12870), lane C - human recombinant PKC-gamma (1-368a.a., N-terminal GST tag, H00006300-P01), lane D - human recombinant p38-delta (1-366a.a., N-terminal GST tag, H00005603-P01). 500ng of recombinant protein was loaded in each well.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-E13-Q-Western-Blot-NBP3-08206-img0001.jpg "Western Blot: p38 beta/MAPK11 Antibody (E13-Q) [NBP3-08206] - Western blot analysis of p38 beta/MAPK11. Lane A - human recombinant p38-alpha (260-360a.a., N-terminal GST tag; H00001432-Q01), lane B - human recombinant p38 beta/MAPK11 (with C-terminal DDK tag, NBL1-12870), lane C - human recombinant PKC-gamma (1-368a.a., N-terminal GST tag, H00006300-P01), lane D - human recombinant p38-delta (1-366a.a., N-terminal GST tag, H00005603-P01). 500ng of recombinant protein was loaded in each well.")
![Immunocytochemistry/Immunofluorescence: p38 beta/MAPK11 Antibody (E13-Q) [NBP3-08206] - Representative picture of p38 beta/MAPK11 expression in HEK293 cells, visualized with clonal rabbit anti-p38 beta/MAPK11 monospecific antibody. Primary antibody dilution - 1:500.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-E13-Q-Immunocytochemistry-Immunofluorescence-NBP3-08206-img0002.jpg)
![Western Blot: p38 beta/MAPK11 Antibody (1F9) [H00005600-M03] - Analysis of MAPK11 expression in transfected 293T cell line by MAPK11 monoclonal antibody (M03), clone 1F9. Lane 1: MAPK11 transfected lysatE (41.4 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-1F9-Western-Blot-H00005600-M03-img0004.jpg "Western Blot: p38 beta/MAPK11 Antibody (1F9) [H00005600-M03] - Analysis of MAPK11 expression in transfected 293T cell line by MAPK11 monoclonal antibody (M03), clone 1F9. Lane 1: MAPK11 transfected lysatE (41.4 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: p38 beta/MAPK11 Antibody (1F9) [H00005600-M03] - Western blot analysis of MAPK11 over-expressed 293 cell line, cotransfected with MAPK11 Validated Chimera RNAi or non-transfected control. Blot probed with H00005600-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-1F9-Western-Blot-H00005600-M03-img0002.jpg)
![ELISA: p38 beta/MAPK11 Antibody (1F9) [H00005600-M03] - Detection limit for recombinant GST tagged MAPK11 is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-1F9-ELISA-H00005600-M03-img0003.jpg)
![Western blot analysis on different lysates with NBP3-33145 at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: K-562 cell lysate (30 µg/Lane) Lane 5: MDA-MB-231 cell lysate (30 µg/Lane) Lane 6: COS-1 cell lysate (30 µg/Lane) Lane 7: RAW264.7 cell lysate (30 µg/Lane) Lane 8: Neuro-2a cell lysate (30 µg/Lane) Lane 9: PC-12 cell lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33145 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33145_-western-blot-639204597482435026.jpg "Western blot analysis on different lysates with NBP3-33145 at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: HeLa cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: K-562 cell lysate (30 µg/Lane) Lane 5: MDA-MB-231 cell lysate (30 µg/Lane) Lane 6: COS-1 cell lysate (30 µg/Lane) Lane 7: RAW264.7 cell lysate (30 µg/Lane) Lane 8: Neuro-2a cell lysate (30 µg/Lane) Lane 9: PC-12 cell lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33145 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 beta/MAPK11 Antibody [NBP2-48826] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells, subset of glial cells and in neuropil.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-Immunohistochemistry-NBP2-48826-img0001.jpg "Immunohistochemistry: p38 beta/MAPK11 Antibody [NBP2-48826] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells, subset of glial cells and in neuropil.")
![Western Blot: MAPK11 Overexpression Lysate (Adult Normal) [NBL1-12870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAPK11.](http://images.novusbio.com/fullsize/MAPK11-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12870-img0002.jpg "Western Blot: MAPK11 Overexpression Lysate (Adult Normal) [NBL1-12870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAPK11.")
![SDS-Page: Recombinant Human p38 beta/MAPK11 His Protein [NBP1-72538] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human p38 beta-MAPK11 His Protein-SDS-Page-NBP1-72538-img0002.jpg "SDS-Page: Recombinant Human p38 beta/MAPK11 His Protein [NBP1-72538] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
