| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1230-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1230-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71233 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02974-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF12301-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87943-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | DYC1230C-5 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | DYC1230C-5 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60506-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26150-50ul | |||
![Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Western-Blot-NBP1-47842-img0011.jpg "Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERK2 monoclonal antibody.")
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Endometrium tissue within the normal limits using antiMAPK1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,Dilution 1:50)](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47842-img0019.jpg)
![Western Blot: ERK2 Antibody (6E5) [NBP1-47842] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERK2.](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Western-Blot-NBP1-47842-img0010.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47842-img0003.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Staining of paraffin-embedded breast tissue using anti-ERK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47842-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-ERK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47842-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Staining of paraffin-embedded endometrium tissue using anti-ERK2mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47842-img0006.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Staining of paraffin-embedded pancreas tissue using anti-ERK2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Immunohistochemistry-Paraffin-NBP1-47842-img0007.jpg)
![Immunoprecipitation: ERK2 Antibody (6E5) [NBP1-47842] - TrueMab monoclonal anti-ERK2 antibody (Negative control: IP without adding anti-ERK2 antibody). For each experiment, 500ul of DDK tagged ERK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of antibody.](http://images.novusbio.com/fullsize/ERK2-Antibody-6E5-Immunoprecipitation-NBP1-47842-img0023.jpg)
![Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ERK2-Antibody-Western-Blot-NBP3-02974-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ERK2 Antibody [NBP3-02974] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02974-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP3-02974] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP3-02974-img0003.jpg)
![Western Blot: ERK2 Antibody [NBP3-02974] - Western blot analysis of extracts of various cell lines, using ERK2 antibody (NBP3-02974) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ERK2 Antibody-Western Blot-NBP3-02974-img0005.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP3-02974] - Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] ERK2 Rabbit pAb (NBP3-02974) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP3-02974-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] - Western blot analysis of lysates from wild type (WT) and ERK2 knockout (KO) HeLa cells using [KO Validated] ERK2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 90 s.](http://images.novusbio.com/fullsize/nbp3-02974_rabbit-polyclonal-erk2-antibody-5220251636384.jpg)
![Western Blot: ERK2 Antibody - Azide and BSA Free [ERK2] - Western blot analysis of various lysates using [KO Validated] ERK2 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 30 s.](http://images.novusbio.com/fullsize/nbp3-02974_rabbit-polyclonal-erk2-antibody-5220251632146.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP1-87943-img0011.jpg "Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP1-87943-img0009.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP1-87943-img0010.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP1-87943-img0012.jpg)
![Simple Western: ERK2 Antibody [NBP1-87943] - Simple Western lane view shows a specific band for ERK2 in 0.2 mg/ml of U-2 OS (left) and h. Pancreas (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ERK2-Antibody-Simple-Western-NBP1-87943-img0007.jpg)
![Simple Western: ERK2 Antibody [NBP1-87943] - Electropherogram image of the corresponding Simple Western lane view. ERK2 antibody was used at 1:5 dilution on U-2 OS and h. Pancreas lysate(s) respectively.](http://images.novusbio.com/fullsize/ERK2-Antibody-Simple-Western-NBP1-87943-img0008.jpg)
![ELISA ERK2 [Biotin]](http://images.novusbio.com/fullsize2/dyc1230c-2_human-mouse-rat-total-erk2-duoset-ic-elisa-2-plate-elisa-169202013352.jpg "ELISA ERK2 [Biotin]")
![Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using ERK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15826_rabbit-erk2-mab-2k9n9-5220251718970.jpg "Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using ERK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using ERK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15826_rabbit-erk2-mab-2k9n9-52202517162369.jpg)
![Immunohistochemistry: ERK2 Antibody (2K9N9) [ERK2] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using ERK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15826_rabbit-erk2-mab-2k9n9-5220251726023.jpg)
![Western Blot: ERK2 Antibody (4C11) [NB110-60506] - Analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.](http://images.novusbio.com/fullsize/ERK2-Antibody-4C11-Western-Blot-NB110-60506-img0010.jpg "Western Blot: ERK2 Antibody (4C11) [NB110-60506] - Analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (4C11) [NB110-60506] - Analysis of Eca-109 cells using ERK2 mouse mAb (green).](http://images.novusbio.com/fullsize/ERK2-Antibody-4C11-Immunocytochemistry-Immunofluorescence-NB110-60506-img0007.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (4C11) [NB110-60506] - Analysis of human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization using ERK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ERK2-Antibody-4C11-Immunohistochemistry-Paraffin-NB110-60506-img0009.jpg)
![Immunohistochemistry: ERK2 Antibody (10C7) [NBP3-26150] - Image of ERK2 Antibody (10C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-262202415475643.jpg "Immunohistochemistry: ERK2 Antibody (10C7) [NBP3-26150] - Image of ERK2 Antibody (10C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: ERK2 Antibody (10C7) [NBP3-26150] - Positive Western Blot detected in: Jurkat whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A375 whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: ERK2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42, 37 kDa Observed band size: 42 kDa](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-2822024114091.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (10C7) [NBP3-26150] - Staining of Hela Cells with ERK2 Antibody (10C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26150_rabbit-erk2-mab-10c7-28220241249366.jpg)
![Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.](http://images.novusbio.com/fullsize/ERK2-Antibody-Western-Blot-NBP1-19102-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/ERK2-Antibody-Immunohistochemistry-Paraffin-NBP1-19102-img0003.jpg)