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![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining in human adrenal gland and kidney tissues using anti-PENK antibody. Corresponding PENK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining in human adrenal gland and kidney tissues using anti-PENK antibody. Corresponding PENK RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Met-enkephalin Antibody [NBP1-90944] - Analysis in control (vector only transfected HEK293T lysate) and PENK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Met-enkephalin-Antibody-Western-Blot-NBP1-90944-img0011.jpg)
![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining of human adrenal gland shows strong cytoplasmic positivity in medullary cells.](http://images.novusbio.com/fullsize/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0008.jpg)
![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0009.jpg)
![Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Human prostate using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241252820.jpg "Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Human prostate using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: Proenkephalin Antibody [NBP3-38239] - Immunofluorescence analysis of paraffin-embedded Human adrenal gland using Proenkephalin Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241252847.jpg)
![Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241224152.jpg)
![Western Blot: Proenkephalin Antibody [NBP3-38239] - Western blot analysis of lysates from THP-1 cells, using Proenkephalin Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-10122024126238.jpg)
![Western Blot: Proenkephalin Antibody (9E7) [H00005179-M04] - Analysis of PENK expression in transfected 293T cell line by PENK monoclonal antibody (M04), clone 9E7. Lane 1: PENK transfected lysate (Predicted MW: 29.48 kDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-9E7-Western-Blot-H00005179-M04-img0002.jpg "Western Blot: Proenkephalin Antibody (9E7) [H00005179-M04] - Analysis of PENK expression in transfected 293T cell line by PENK monoclonal antibody (M04), clone 9E7. Lane 1: PENK transfected lysate (Predicted MW: 29.48 kDa). Lane 2: Non-transfected lysate.")
![Sandwich ELISA: Proenkephalin Antibody (9E7) [H00005179-M04] - Detection limit for recombinant GST tagged PENK is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-9E7-Sandwich-ELISA-H00005179-M04-img0001.jpg)
![Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-Immunohistochemistry-NBP1-20963-img0003.jpg "Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.")
![Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Mouse Stria terminalis. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-Immunohistochemistry-NBP1-20963-img0001.jpg)
![Western Blot: Proenkephalin Antibody [NBP1-06066] - (0.3ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-Western-Blot-NBP1-06066-img0002.jpg "Western Blot: Proenkephalin Antibody [NBP1-06066] - (0.3ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-06066] - Section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-Immunohistochemistry-Frozen-NBP1-06066-img0003.jpg)
![Western Blot: Proenkephalin Antibody [NBP1-59326] - Jurkat cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-Western-Blot-NBP1-59326-img0002.jpg "Western Blot: Proenkephalin Antibody [NBP1-59326] - Jurkat cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Proenkephalin Overexpression Lysate (Adult Normal) [NBL1-14289] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proenkephalin.](http://images.novusbio.com/fullsize/Proenkephalin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14289-img0001.jpg "Western Blot: Proenkephalin Overexpression Lysate (Adult Normal) [NBL1-14289] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proenkephalin.")
![Western Blot: Proenkephalin Overexpression Lysate (Adult Normal) [NBP2-11091] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proenkephalin.](http://images.novusbio.com/fullsize/Proenkephalin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11091-img0001.jpg "Western Blot: Proenkephalin Overexpression Lysate (Adult Normal) [NBP2-11091] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proenkephalin.")
![Immunohistochemistry-Paraffin: Proenkephalin Antibody [NBP3-14448] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Proenkephalin Antibody-Immunohistochemistry-Paraffin-NBP3-14448-img0001.jpg "Immunohistochemistry-Paraffin: Proenkephalin Antibody [NBP3-14448] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Immunohistochemistry-Paraffin: Proenkephalin Antibody [NBP3-14447] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Proenkephalin Antibody-Immunohistochemistry-Paraffin-NBP3-14447-img0001.jpg "Immunohistochemistry-Paraffin: Proenkephalin Antibody [NBP3-14447] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)")
