Hu,
Mu,
Rt,
Po,
Am,
Bv,
RbSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Am, Bv, Rb
WB, ELISA, Flow, ICC/IF, IHC, Mycoplasma
Applications: WB, ELISA, Flow, ICC/IF, IHC, Mycoplasma
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.](http://images.novusbio.com/fullsize/PMCA4-Antibody-JA9-Immunocytochemistry-Immunofluorescence-NB300-569-img0005.jpg "Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.")
![Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569] - Normal biopsies of deparaffinized Human brain tissue.](http://images.novusbio.com/fullsize/PMCA4-Antibody-JA9-Immunohistochemistry-Paraffin-NB300-569-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Figure 1 illustrates immunolocalization of PMCA4 and CaSRO in human corneal epithelium. (A) Immunolocalization of PMCA4 (green) using NB300-569 on cell membranes of all cells in each layer of hCE. (B) Immunolocalization of CaSRO (red)on cell membranes of hCE. (C) Merged image. Figure 2 illustrates immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.](http://images.novusbio.com/fullsize/PMCA4-Antibody-JA9-Immunocytochemistry-Immunofluorescence-NB300-569-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.](http://images.novusbio.com/fullsize/PMCA4-Antibody-JA9-Immunocytochemistry-Immunofluorescence-NB300-569-img0004.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. Endogenous expression of PMCA4b in WT and renalase KO mice, western immunoblot using an anti-renalase monoclonal antibody; 10 ug protein loaded in each lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Western-Blot-H00000493-M07-img0005.jpg "Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. Endogenous expression of PMCA4b in WT and renalase KO mice, western immunoblot using an anti-renalase monoclonal antibody; 10 ug protein loaded in each lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (2G8) [H00000493-M07] - Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein. Co-immunolocalization of PMCA4b and renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 um; arrow = plasma membrane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Immunocytochemistry-Immunofluorescence-H00000493-M07-img0006.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein. Co-Immunoprecipitation of PMCA4b and renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Western-Blot-H00000493-M07-img0002.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. siRNA mediated inhibition of PMCA4b expression downregulates RP-220 mediated MAPK signaling; left panel: RP-220 mediated ERK and p38 activation in HK-2 cells transfected with non-targeting siRNA, p = phosphorylated, representative immunoblot; middle panel: Inhibition of RP-220 mediated ERK and p38 activation in HK-2 cells transfected with PMCA4b siRNA, representative blot; right panel: quantification of phosphorylated ERK (p-ERK), signals normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control; n = 3, * = P<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Western-Blot-H00000493-M07-img0003.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. Lack of effect of siRNA mediated inhibition of PMCA4b expression on epidermal growth factor (EGF)- mediated MAPK signaling; left panel: EGF (100 ng/ml) mediated ERK, p38 activation and c-Jun N-Terminal Kinase (JNK) in HK-2 cells transfected with non-targeting siRNA, p = phosphorylated, representative blot; right panel: EGF-mediated ERK, p38 and JNK activation in HK-2 cells unaffected by transfection with PMCA4b siRNA and downregulation of PMCA4b expression; representative blot (n = 3). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Western-Blot-H00000493-M07-img0004.jpg)
![Sandwich ELISA: PMCA4 Antibody (2G8) [H00000493-M07] - Detection limit for recombinant GST tagged ATP2B4 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Sandwich-ELISA-H00000493-M07-img0001.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein.A, HK-2 cells incubated with either labeled RP-Scr220 or RP-220, biotin-labeled proteins purified using streptavidin column, separated by SDS-PAGE & visualized by western blot using streptavidin-HRP; * = regions evaluated by mass spectrometry in samples labeled with either RP-Scr220 or RP-220; # = RP-220 band containing the plasma membrane calcium ATPase isoform PMCA4b. B, Endogenous expression of PMCA4b in HK-2 cells, western immunoblot using isoform specific monoclonal; CCL-119: human leukemic cell line; thyroid tumor = human thyroid tumor cell line (ATCC, CRL-1803) 10 μg protein loaded in each lane. C, co-immunolocalization of PMCA4b & renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 μm; arrow = plasma membrane. D, Co-Immunoprecipitation of PMCA4b & renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25906147), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000493-m07_mouse-monoclonal-pmca4-antibody-2g8-310202415482242.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody [NBP2-31984] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31984-img0005.jpg "Immunocytochemistry/Immunofluorescence: PMCA4 Antibody [NBP2-31984] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMCA4 Antibody [NBP2-31984] - Staining in human fallopian tube and pancreas tissues using anti-ATP2B4 antibody. Corresponding ATP2B4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Immunohistochemistry-Paraffin-NBP2-31984-img0008.jpg)
![Immunohistochemistry-Paraffin: PMCA4 Antibody [NBP2-31984] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Immunohistochemistry-Paraffin-NBP2-31984-img0006.jpg)
![Immunohistochemistry-Paraffin: PMCA4 Antibody [NBP2-31984] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Immunohistochemistry-Paraffin-NBP2-31984-img0007.jpg)
![Western Blot: PMCA4 Antibody [NBP1-59481] - 721_B cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Western-Blot-NBP1-59481-img0002.jpg "Western Blot: PMCA4 Antibody [NBP1-59481] - 721_B cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: PMCA4 Overexpression Lysate (Adult Normal) [NBP2-10766] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PMCA4.](http://images.novusbio.com/fullsize/PMCA4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10766-img0001.jpg "Western Blot: PMCA4 Overexpression Lysate (Adult Normal) [NBP2-10766] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PMCA4.")
![Western Blot: PMCA4 Antibody [H00000493-D01P] - Analysis of ATP2B4 expression in human colon.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Western-Blot-H00000493-D01P-img0001.jpg "Western Blot: PMCA4 Antibody [H00000493-D01P] - Analysis of ATP2B4 expression in human colon.")