CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcolemma of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Nidogen‑1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Nidogen‑1/Entactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2570) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcolemma of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2570-SP | |||
HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Nidogen‑1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Nidogen‑1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2570-SP | |||
| Carrier Free | MAB2570-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08237 | |||
2570-ND) was serially diluted and captured by Mouse Anti-Human Nidogen‑1/Entactin Monoclonal Antibody (Catalog # 
NL007) and counterstained with DAPI (blue). View our protocol for 
2570-ND) was serially diluted and captured by Mouse Anti-Human Nidogen‑1/Entactin Monoclonal Antibody (Catalog # MAB2570) coated on a Clear Polystyrene Microplate (Catalog # ![N/A Nidogen-1/Entactin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Nidogen1_DY2570_ELISA_1849.jpg "N/A Nidogen-1/Entactin [Biotin]")
![Western Blot: Nidogen-1/Entactin Antibody [NBP2-16341] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Entactin-Antibody-Western-Blot-NBP2-16341-img0001.jpg "Western Blot: Nidogen-1/Entactin Antibody [NBP2-16341] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Nidogen-1/Entactin Antibody [NBP2-16341] - Human liver carcinoma, using Nidogen antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Entactin-Antibody-Immunohistochemistry-Paraffin-NBP2-16341-img0002.jpg)
![Western Blot: Nidogen-1/Entactin Antibody (S08-3H3) [NBP3-19860] - Western blot detection of Nidogen-1/Entactin in Hela (Lane 1), A549 (Lane 2), HL-60 (Lane 3), U251 (Lane 4), U87-MG (Lane 5) cell lysates using NBP3-19860 (1:1000 diluted). Predicted band size: 136kDa. Observed band size: 136kDa.](http://images.novusbio.com/fullsize/Nidogen-1-Entactin-Antibody-S08-3H3-Western-Blot-NBP3-19860-img0002.jpg "Western Blot: Nidogen-1/Entactin Antibody (S08-3H3) [NBP3-19860] - Western blot detection of Nidogen-1/Entactin in Hela (Lane 1), A549 (Lane 2), HL-60 (Lane 3), U251 (Lane 4), U87-MG (Lane 5) cell lysates using NBP3-19860 (1:1000 diluted). Predicted band size: 136kDa. Observed band size: 136kDa.")
![Immunohistochemistry-Paraffin: Nidogen-1/Entactin Antibody (S08-3H3) [NBP3-19860] - Immunohistochemistry of Nidogen-1/Entactin in paraffin-embedded Human colon cancer tissue using NBP3-19860 at dilution 1/50](http://images.novusbio.com/fullsize/Nidogen-1-Entactin-Antibody-S08-3H3-Immunohistochemistry-Paraffin-NBP3-19860-img0001.jpg)
![Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01] - Analysis of NID1 expression in transfected 293T cell line by NID1 monoclonal antibody (M01), clone 1G3.Lane 1: NID1 transfected lysate(122.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Nidogen-1-Entactin-Antibody-1G3-Western-Blot-H00004811-M01-img0003.jpg "Western Blot: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01] - Analysis of NID1 expression in transfected 293T cell line by NID1 monoclonal antibody (M01), clone 1G3.Lane 1: NID1 transfected lysate(122.1 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: Nidogen-1/Entactin Antibody (1G3) [H00004811-M01] - Detection limit for recombinant GST tagged NID1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nidogen-1-Entactin-Antibody-1G3-Sandwich-ELISA-H00004811-M01-img0002.jpg)
![Western Blot: Entactin Overexpression Lysate (Adult Normal) [NBL1-13641] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Entactin.](http://images.novusbio.com/fullsize/Entactin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13641-img0002.jpg "Western Blot: Entactin Overexpression Lysate (Adult Normal) [NBL1-13641] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Entactin.")
