HAF016). A specific band was detected for p67phox at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and human immature dendritic cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human p67phox Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7830) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for p67phox at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7830-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94271-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97097-50ul | |||
![Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Analysis in human bone marrow and skeletal muscle tissues. Corresponding p67phox/NOXA2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP1-82542-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Analysis in human bone marrow and skeletal muscle tissues. Corresponding p67phox/NOXA2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP1-82542-img0017.jpg)
![Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP1-82542-img0014.jpg)
![Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP1-82542-img0015.jpg)
![Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82542] - Staining of human placenta shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP1-82542-img0016.jpg)
![Western Blot: p67phox/NOXA2 Antibody [NBP1-82543] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Western-Blot-NBP1-82543-img0004.jpg "Western Blot: p67phox/NOXA2 Antibody [NBP1-82543] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP1-82543-img0005.jpg)
![Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP1-82543-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP1-82543] - Staining in human bone marrow and skeletal muscle tissues using anti-NCF2 antibody. Corresponding NCF2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP1-82543-img0006.jpg)
![Western Blot: p67phox/NOXA2 Antibody [NBP2-94271] - Analysis of extracts of various cell lines, using p67phox/NOXA2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Western-Blot-NBP2-94271-img0003.jpg "Western Blot: p67phox/NOXA2 Antibody [NBP2-94271] - Analysis of extracts of various cell lines, using p67phox/NOXA2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody [NBP2-94271] - Immunohistochemistry of paraffin-embedded human esophageal cancer using p67phox/NOXA2 Rabbit pAb (NBP2-94271) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunohistochemistry-Paraffin-NBP2-94271-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: p67phox/NOXA2 Antibody - BSA Free [p67phox/NOXA2] - Immunofluorescence analysis of Human spleen tissue using p67phox/NOXA2 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp2-94271_rabbit-polyclonal-p67phox-noxa2-antibody-5220251640238.jpg)
![Western Blot: p67phox/NOXA2 Antibody (7J7N3) [NBP3-16257] - Western blot analysis of extracts of various cell lines, using p67phox/NOXA2 Rabbit mAb (NBP3-16257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-7J7N3-Western-Blot-NBP3-16257-img0002.jpg "Western Blot: p67phox/NOXA2 Antibody (7J7N3) [NBP3-16257] - Western blot analysis of extracts of various cell lines, using p67phox/NOXA2 Rabbit mAb (NBP3-16257) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: p67phox/NOXA2 Antibody (7J7N3) [NBP3-16257] - Immunohistochemistry of paraffin-embedded mouse kidney using p67phox/NOXA2 Rabbit mAb (NBP3-16257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-7J7N3-Immunohistochemistry-Paraffin-NBP3-16257-img0001.jpg)
![Western Blot: p67phox/NOXA2 Antibody [NBP2-97097] - Anti-p67phox/NOXA2 rabbit polyclonal antibody at 1:500 dilution. Lane A: THP1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Western-Blot-NBP2-97097-img0004.jpg "Western Blot: p67phox/NOXA2 Antibody [NBP2-97097] - Anti-p67phox/NOXA2 rabbit polyclonal antibody at 1:500 dilution. Lane A: THP1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa")
![Immunoprecipitation: p67phox/NOXA2 Antibody [NBP2-97097] - p67phox/NOXA2 was immunoprecipitated using: Lane A: 0.5 mg THP-1 Whole Cell Lysate. 2 ul anti-p67phox/NOXA2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-p67phox/NOXA2 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa](http://images.novusbio.com/fullsize/p67phox-NOXA2-Antibody-Immunoprecipitation-NBP2-97097-img0003.jpg)
![SDS-Page: Recombinant Human p67phox/NOXA2 GST (N-Term) Protein [H00004688-P01] - Recombinant Human p67phox/NOXA2 Protein [H00004688-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004688-P01-1.jpg "SDS-Page: Recombinant Human p67phox/NOXA2 GST (N-Term) Protein [H00004688-P01] - Recombinant Human p67phox/NOXA2 Protein [H00004688-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: NOXA2 Overexpression Lysate (Adult Normal) [NBL1-13510] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOXA2.](http://images.novusbio.com/fullsize/NOXA2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13510-img0002.jpg "Western Blot: NOXA2 Overexpression Lysate (Adult Normal) [NBL1-13510] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOXA2.")
![Western Blot: NOXA2 Overexpression Lysate (Adult Normal) [NBP2-11127] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOXA2.](http://images.novusbio.com/fullsize/NOXA2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11127-img0001.jpg "Western Blot: NOXA2 Overexpression Lysate (Adult Normal) [NBP2-11127] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOXA2.")
