| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71703SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89400-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89399-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58516-25ul | |||
![Western Blot: NBR1 Antibody (6B11) [H00004077-M01] - Western Blot analysis of NBR1 expression in PC-12](http://images.novusbio.com/fullsize/NBR1-Antibody-6B11-Western-Blot-H00004077-M01-img0001.jpg "Western Blot: NBR1 Antibody (6B11) [H00004077-M01] - Western Blot analysis of NBR1 expression in PC-12")
![Immunocytochemistry/Immunofluorescence: NBR1 Antibody (6B11) [H00004077-M01] - Top panel: HeLa cells stably expressing EGFP-Parkin were treated with 10 uM G-TPP and fixed 8 h after treatment. Cells were stained with antibodies against the autophagy adapter protein NBR1 (red), mitochondria were counterstained with TOM20 antibodies (cyan), nuclei with Hoechst (blue). EGFP-Parkin epifluorescence is shown in green. Scale bar corresponds to 10 uM. Bottom panel: Human fibroblasts were treated with 15 uM G-TPP for 16 h and fixed and stained with antibodies against the autophagy adapter NBR (green), mitochondria were stained with antibodies against TOM20 (red), nuclei were visualized with Hoechst (blue). Scale bars indicate 10 uM. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NBR1-Antibody-6B11-Immunocytochemistry-Immunofluorescence-H00004077-M01-img0007.jpg)
![ELISA: NBR1 Antibody (6B11) [H00004077-M01] - Detection limit for recombinant GST tagged NBR1 is approximately 0.1 ng/mL as a capture antibody.](http://images.novusbio.com/fullsize/NBR1-Antibody-6B11-ELISA-H00004077-M01-img0004.jpg)
![Simple Western: NBR1 Antibody (6B11) [H00004077-M01] - This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown) by Simple Western. Results from two hybridoma clones are shown. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NBR1-Antibody-6B11-Simple-Western-H00004077-M01-img0005.jpg)
![Western Blot: NBR1 Antibody (6B11) [H00004077-M01] - G-TPP activity is conserved in primary fibroblasts(A, C) Fibroblasts were treated with 15 µM G-TPP for the indicated time points. Cells were harvested & western blots were probed with antibodies against (A) PINK1, pS65-Ub & total Ub or (C) autophagy adapter proteins. GAPDH & Vinculin served as loading control. G-TPP treatment led to PINK1 stabilization & pS65-Ub induction in primary skin fibroblasts. p62 levels were induced upon G-TPP treatment, while other adapters seemed decreased. (B, D) Human fibroblasts were treated with 15 µM G-TPP for 16 h & fixed & stained with antibodies against (B) pS65-Ub (green) or (D) the autophagy adapters NBR1, NDP52, p62, OPTN & TAX1BP1 (green). Mitochondria were stained with antibodies against TOM20 (red), nuclei were visualized with Hoechst (blue). Scale bars indicate 10 µM. A magnified image of the boxed region, the fluorescence profile along the arrow & the Pearson’s correlation coefficient of adapter protein & mitochondrial stainingare shown to the right. Shown is the mean ± SEM of at least five randomly selected images (unpaired, two-sided t-test, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004077-m01_mouse-monoclonal-nbr1-antibody-6b11-310202415485138.jpg)
![Immunocytochemistry/ Immunofluorescence: NBR1 Antibody (6B11) [H00004077-M01] - G-TPP leads to recruitment of autophagy adapters & degradation of mitochondria(A) HeLa cells stably expressing untagged Parkin were treated with 10 µM G-TPP for 8 h. Western blots were prepared from cell lysates & probed with antibodies against LC3, phospho-TBK1 (Ser172) & TBK1. GAPDH was used as a loading control. Upon 8 h the levels of LC3-I & LC3-II were both increased. At 8 h after treatment with G-TPP but not at 4 or 24 h, TBK1 was phosphorylated. (B) HeLa cells stably expressing EGFP-Parkin were treated with 10 µM G-TPP & fixed 8 h after treatment. Cells were stained with antibodies against the autophagy adapter proteins NBR1, NDP52, OPTN, p62, & TAX1BP1 (red). Mitochondria were counterstained with TOM20 antibodies (cyan), nuclei with Hoechst (blue). EGFP-Parkin epifluorescence is shown in green. Scale bar corresponds to 10 µM. (C) HeLa cells stably expressing EGFP-Parkin & the reporter protein mitoKeima were treated with 10 µM CCCP or G-TPP & imaged over time. The ratio of ‘neutral’ mitoKeima to ‘acidic’ mitoKeima was calculated as readout for mitophagy. Parkin translocation was monitored at the same time. Values for Parkin translocation & mitophagy were normalized to 12 h treatment with 10 µM CCCP as positive control & DMSO as negative control (two-way ANOVA with Tukey’s post-hoc test, **p < 0.005, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004077-m01_mouse-monoclonal-nbr1-antibody-6b11-310202415482269.jpg)
![Immunocytochemistry/ Immunofluorescence: NBR1 Antibody (6B11) [H00004077-M01] - G-TPP activity is conserved in primary fibroblasts(A, C) Fibroblasts were treated with 15 µM G-TPP for the indicated time points. Cells were harvested & western blots were probed with antibodies against (A) PINK1, pS65-Ub & total Ub or (C) autophagy adapter proteins. GAPDH & Vinculin served as loading control. G-TPP treatment led to PINK1 stabilization & pS65-Ub induction in primary skin fibroblasts. p62 levels were induced upon G-TPP treatment, while other adapters seemed decreased. (B, D) Human fibroblasts were treated with 15 µM G-TPP for 16 h & fixed & stained with antibodies against (B) pS65-Ub (green) or (D) the autophagy adapters NBR1, NDP52, p62, OPTN & TAX1BP1 (green). Mitochondria were stained with antibodies against TOM20 (red), nuclei were visualized with Hoechst (blue). Scale bars indicate 10 µM. A magnified image of the boxed region, the fluorescence profile along the arrow & the Pearson’s correlation coefficient of adapter protein & mitochondrial stainingare shown to the right. Shown is the mean ± SEM of at least five randomly selected images (unpaired, two-sided t-test, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004077-m01_mouse-monoclonal-nbr1-antibody-6b11-310202415482210.jpg)
![Western Blot: NBR1 Antibody [NBP1-71703] - The antibody detects HeLa cell NBR1 (about 130 kDa). It detects a 100 kDa band in mouse brain. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NBR1-Antibody-Western-Blot-NBP1-71703-img0011.jpg "Western Blot: NBR1 Antibody [NBP1-71703] - The antibody detects HeLa cell NBR1 (about 130 kDa). It detects a 100 kDa band in mouse brain. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: NBR1 Antibody [NBP1-71703] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with NBR1 Antibody (NBP1-71703) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71703-img0012.jpg)
![Immunohistochemistry: NBR1 Antibody [NBP1-71703] - Staining of NBRI in mouse prostate.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunohistochemistry-NBP1-71703-img0006.jpg)
![Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of NBR1 in HepG2 cell lysates.](http://images.novusbio.com/fullsize/NBR1-Antibody-Western-Blot-NBP1-71703-img0007.jpg)
![Western Blot: NBR1 Antibody [NBP1-71703] - WB analysis of 30 ug whole cell lysate of HeLa cells using NBR1 antibody.](http://images.novusbio.com/fullsize/NBR1-Antibody-Western-Blot-NBP1-71703-img0010.jpg)
![Simple Western: NBR1 Antibody [NBP1-71703] - Simple Western lane view shows a specific band for NBR1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NBR1-Antibody-Simple-Western-NBP1-71703-img0008.jpg)
![Immunohistochemistry: NBR1 Antibody [NBP1-89399] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunohistochemistry-NBP1-89399-img0002.jpg "Immunohistochemistry: NBR1 Antibody [NBP1-89399] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.")
![Immunocytochemistry/Immunofluorescence: NBR1 Antibody [NBP2-58516] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/NBR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58516-img0001.jpg "Immunocytochemistry/Immunofluorescence: NBR1 Antibody [NBP2-58516] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles.")
![Immunoprecipitation:NBR1 AntibodyNBP3-30062] -Detection of human NBR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NBR1 antibody NBP3-30062 (lot NBP3-30062-1) used for IP at 6 µg per reaction. NBR1 was also immunoprecipitated by rabbit anti-NBR1 antibody . For blotting immunoprecipitated NBR1, NBP3-30062 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30062_rabbit-nbr1-pab-301220241630344.jpg "Immunoprecipitation:NBR1 AntibodyNBP3-30062] -Detection of human NBR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NBR1 antibody NBP3-30062 (lot NBP3-30062-1) used for IP at 6 µg per reaction. NBR1 was also immunoprecipitated by rabbit anti-NBR1 antibody . For blotting immunoprecipitated NBR1, NBP3-30062 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: NBR1 Overexpression Lysate (Adult Normal) [NBP2-06115] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NBR1.](http://images.novusbio.com/fullsize/NBR1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06115-img0001.jpg "Western Blot: NBR1 Overexpression Lysate (Adult Normal) [NBP2-06115] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NBR1.")
![Western Blot: NBR1 Overexpression Lysate (Adult Normal) [NBP2-06113] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NBR1.](http://images.novusbio.com/fullsize/NBR1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06113-img0001.jpg "Western Blot: NBR1 Overexpression Lysate (Adult Normal) [NBP2-06113] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NBR1.")
![Western Blot: NBR1 Overexpression Lysate (Adult Normal) [NBP2-07691] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NBR1.](http://images.novusbio.com/fullsize/NBR1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07691-img0001.jpg "Western Blot: NBR1 Overexpression Lysate (Adult Normal) [NBP2-07691] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NBR1.")