| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1154-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71124 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43313-0.5mg | |||
| BSA Free | NBP1-43313-0.05mg | |||
| BSA Free | NBP1-43313-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03252-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC11542P | |||
| Carrier Free | MAB11542-SP | |||
| Carrier Free | IC11542V-100UG | |||
| Carrier Free | IC11542U-100UG | |||
| Carrier Free | IC11542T-100UG | |||
| Carrier Free | IC11542S-100UG | |||
| Carrier Free | IC11542R-100UG | |||
| Carrier Free | IC11542N-100UG | |||
| Carrier Free | IC11542G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89383-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34881-1000ug | |||
| Carrier-Free | NBP2-34881-100ug | |||
| Carrier-Free | NBP2-34881-500ug | |||
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16590] - Immunohistochemical analysis of paraffin-embedded Gastric carcinoma, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16590-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0001.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysate B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0002.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - A. 50 ug rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16590] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0005.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16590] - Sandwich ELISA detection of recombinant full-length Galectin 3 protein using as detection antibody at concentration of 1 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16590-img0006.jpg)
![Western Blot: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Galectin-3.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Western-Blot-NBP2-45618-img0022.jpg "Western Blot: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Galectin-3.")
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0021.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0001.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0002.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0003.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0004.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0005.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0006.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0007.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0008.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0009.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0010.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0011.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0012.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0013.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0014.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0015.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0016.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0017.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0018.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0019.jpg)
![Immunohistochemistry: Galectin-3 Antibody (1C7) [NBP2-45618] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Galectin-3-Antibody-1C7-Immunohistochemistry-NBP2-45618-img0020.jpg)
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.. Using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0005.jpg "Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis.. Using the Biotin format of this antibody.")
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - An intracellular stain was performed on RAW 246.7 cells with Galectin-3 Antibody (M3/38) NBP1-43313AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0003.jpg)
![Flow (Intracellular): Galectin-3 Antibody (M3/38) [NBP1-43313] - Intracellular staining of the monocyte population in normal human peripheral blood cells (right) or C57BL/6 mouse bone marrow cells (left) with Anti-Mouse CD11b APC and 0.125 ug of Anti-Human/Mouse Galectin-3 Biotin followed by Streptavidin PE. Total viable cells were used for analysis. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-M3-38-Flow-Intracellular-NBP1-43313-img0004.jpg)
![Western Blot: Galectin-3 Antibody [NBP3-03252] - Analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using Galectin-3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Knockout-Validated-NBP3-03252-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP3-03252] - Analysis of extracts from normal (control) and LGALS3 knockout (KO) HeLa cells, using Galectin-3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03252_rabbit-polyclonal-galectin-3-antibody-5220251628098.jpg)
![Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of C6 cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03252_rabbit-polyclonal-galectin-3-antibody-52202516293915.jpg)
![Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody - Azide and BSA Free [Galectin-3] - Immunofluorescence analysis of L-929 cells using [KO Validated] Galectin-3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03252_rabbit-polyclonal-galectin-3-antibody-52202516345261.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining in human colon and liver tissues using NBP1-89795 antibody. Corresponding LGALS3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-89795-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining in human colon and liver tissues using NBP1-89795 antibody. Corresponding LGALS3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-89795-img0011.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-89795-img0013.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-89795-img0012.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-89795-img0014.jpg)
![Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641] - Wild-type (WT) and Galectin-3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-GT12712-Western-Blot-NBP3-13641-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641] - Wild-type (WT) and Galectin-3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (GT12712) [NBP3-13641] - Galectin-3 antibody [GT12712] detects Galectin-3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Galectin-3 stained by Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-GT12712-Immunocytochemistry-Immunofluorescence-NBP3-13641-img0002.jpg)
![Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-GT12712-Western-Blot-NBP3-13641-img0001.jpg)
![Sandwich ELISA: Galectin-3 Antibody (GT12712) [NBP3-13641] - Sandwich ELISA detection of recombinant full-length Galectin-3 protein using NBP3-13641 as capture antibody at concentration of 5 ug/mL and NBP2-16590 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-GT12712-Sandwich-ELISA-NBP3-13641-img0003.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (GT12712) [NBP3-13641] - Galectin-3 antibody [GT12712] detects Galectin-3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon.Galectin-3 stained by Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13641_mouse-galectin-3-mab-gt12712-9102024201143.jpg)
![Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Western-Blot-NBP2-89383-img0003.jpg "Western Blot: Galectin-3 Antibody (024) [NBP2-89383] - Lane A: HeLa Whole Cell Lysate Lane B: SW480 Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:26 kDa Observed band size:30 kDa")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (024) [NBP2-89383] - Staining of Human Galectin3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human Galectin3 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Immunocytochemistry-Immunofluorescence-NBP2-89383-img0001.jpg)
![Flow Cytometry: Galectin-3 Antibody (024) [NBP2-89383] - Analysis of Human Galectin3 expression on HeLa cells. The cells were treated stained with purified anti-Human Galectin3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Flow-Cytometry-NBP2-89383-img0002.jpg)
![Immunoprecipitation: Galectin-3 Antibody (024) [NBP2-89383] - Lane A:0.5 mg mouse pancreas Whole Cell Lysate 4 uL anti-NKX3-1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody: Anti-NKX3-1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique. Performed under reducing conditions.Predicted band size: 27 kDa Observed band size :30 kDa](http://images.novusbio.com/fullsize/Galectin-3-Antibody-024-Immunoprecipitation-NBP2-89383-img0004.jpg)
![SDS-Page: Human Galectin-3 Protein [NBP2-34881]](http://images.novusbio.com/fullsize/Human-Galectin-3-Protein-SDS-Page-NBP2-34881-img0001.jpg "SDS-Page: Human Galectin-3 Protein [NBP2-34881]")