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![Immunohistochemistry-Paraffin: LASP1 Antibody [NBP1-82844] - Staining in human small intestine and skeletal muscle tissues using anti-LASP1 antibody. Corresponding LASP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LASP1-Antibody-Immunohistochemistry-Paraffin-NBP1-82844-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LASP1 Antibody [NBP1-82844] - Staining in human small intestine and skeletal muscle tissues using anti-LASP1 antibody. Corresponding LASP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LASP1 Antibody [NBP1-82844] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/LASP1-Antibody-Western-Blot-NBP1-82844-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: LASP1 Antibody [NBP1-82844] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LASP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82844-img0009.jpg)
![Immunohistochemistry-Paraffin: LASP1 Antibody [NBP1-82844] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/LASP1-Antibody-Immunohistochemistry-Paraffin-NBP1-82844-img0011.jpg)
![Western Blot: LASP1 Antibody [NBP1-82844] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/LASP1-Antibody-Western-Blot-NBP1-82844-img0007.jpg)
![Immunohistochemistry-Paraffin: LASP1 Antibody [NBP1-82844] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/LASP1-Antibody-Immunohistochemistry-Paraffin-NBP1-82844-img0010.jpg)
![Western Blot: LASP1 Antibody [NBP3-38061] - Western blot analysis of various lysates using LASP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38061_rabbit-lasp1-pab-91220241519570.jpg "Western Blot: LASP1 Antibody [NBP3-38061] - Western blot analysis of various lysates using LASP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: LASP1 Antibody [NBP3-38061] - Immunofluorescence analysis of U-2 OS cells using LASP1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38061_rabbit-lasp1-pab-912202414163026.jpg)
![Immunoprecipitation: LASP1 Antibody [NBP3-38061] - Immunoprecipitation analysis of 300 ug extracts of A-549 cells using 3 ug LASP1 antibody. Western blot was performed from the immunoprecipitate using LASP1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38061_rabbit-lasp1-pab-912202414123888.jpg)
![Immunocytochemistry/ Immunofluorescence: LASP1 Antibody [NBP3-38061] - Immunofluorescence analysis of L929 cells using LASP1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38061_rabbit-lasp1-pab-912202415175273.jpg)
![Immunocytochemistry/ Immunofluorescence: LASP1 Antibody [NBP3-38061] - Immunofluorescence analysis of C6 cells using LASP1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38061_rabbit-lasp1-pab-912202415133462.jpg)
![Western Blot: LASP1 Antibody [NB100-77338] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/LASP1-Antibody-Western-Blot-NB100-77338-img0005.jpg "Western Blot: LASP1 Antibody [NB100-77338] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/LASP1-Antibody-Immunohistochemistry-Paraffin-NB100-77338-img0006.jpg)
![Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338] - (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/LASP1-Antibody-Immunohistochemistry-Paraffin-NB100-77338-img0004.jpg)
![Immunohistochemistry:[NBP3-29558]-Detection of humSP1 by immunohistochemistry. Sl sections of humst c. ffinity purified rnti-Lt. No. Lot1 used dilution of 1: 1,000 Lot1 used dilution of 1: 200. Detection: Din: Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-29558_rabbit-lasp1-pab-2312025128847.jpg "Immunohistochemistry:[NBP3-29558]-Detection of humSP1 by immunohistochemistry. Sl sections of humst c. ffinity purified rnti-Lt. No. Lot1 used dilution of 1: 1,000 Lot1 used dilution of 1: 200. Detection: Din: Hematoxylin.")
![Immunohistochemistry:[NBP3-29651]-Detection of human LASP1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-LASP1 used at a dilution of 1: 1,000 used at a dilution of 1: 200. Detection: DAB. Counterstain: Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-29651_rabbit-lasp1-pab-23120251204018.jpg "Immunohistochemistry:[NBP3-29651]-Detection of human LASP1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-LASP1 used at a dilution of 1: 1,000 used at a dilution of 1: 200. Detection: DAB. Counterstain: Hematoxylin.")
![Western Blot: LASP1 Antibody [NB100-854] - (0.1ug/ml) of Human Brain (Cerebellum) RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/LASP1-Antibody-Western-Blot-NB100-854-img0001.jpg "Western Blot: LASP1 Antibody [NB100-854] - (0.1ug/ml) of Human Brain (Cerebellum) RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: LASP1 Antibody [NBP3-09329] - Immunohistochemical analysis of formalin-fixed paraffin-embedded human heart tissue.](http://images.novusbio.com/fullsize/LASP1-Antibody-Immunohistochemistry-Paraffin-NBP3-09329-img0001.jpg "Immunohistochemistry-Paraffin: LASP1 Antibody [NBP3-09329] - Immunohistochemical analysis of formalin-fixed paraffin-embedded human heart tissue.")
![Western Blot: LASP1 Antibody [NBP2-19334] - Western Blot analysis of LASP1 expression in transfected 293T cell line by LASP1 polyclonal antibody. A: Non-transfected lysate. B: LASP1 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/LASP1-Antibody-Western-Blot-NBP2-19334-img0001.jpg "Western Blot: LASP1 Antibody [NBP2-19334] - Western Blot analysis of LASP1 expression in transfected 293T cell line by LASP1 polyclonal antibody. A: Non-transfected lysate. B: LASP1 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Western Blot: LASP1 Antibody [NBP1-53148] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/LASP1-Antibody-Western-Blot-NBP1-53148-img0002.jpg "Western Blot: LASP1 Antibody [NBP1-53148] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.")
![Western Blot: LASP1 Overexpression Lysate (Adult Normal) [NBP2-07659] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LASP1.](http://images.novusbio.com/fullsize/LASP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07659-img0001.jpg "Western Blot: LASP1 Overexpression Lysate (Adult Normal) [NBP2-07659] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LASP1.")
![Western Blot: LASP1 Antibody (4F5) [H00003927-M05] - analysis of LASP1 expression in transfected 293T cell line by LASP1 monoclonal antibody (M05), clone 4F5. Lane 1: LASP1 transfected lysate (29.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LASP1-Antibody-4F5-Western-Blot-H00003927-M05-img0001.jpg "Western Blot: LASP1 Antibody (4F5) [H00003927-M05] - analysis of LASP1 expression in transfected 293T cell line by LASP1 monoclonal antibody (M05), clone 4F5. Lane 1: LASP1 transfected lysate (29.7 KDa). Lane 2: Non-transfected lysate.")
![SDS-Page: Recombinant Human LASP1 Protein [NBP1-78885] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-LASP1-Protein-SDS-Page-NBP1-78885-img0001.jpg "SDS-Page: Recombinant Human LASP1 Protein [NBP1-78885] - 15% SDS-PAGE (3ug)")