F0108). Quadrant markers were set based Goat IgG Control Antibody staining (Catalog #
AB-108-C, data not shown). View our protocol for
Staining Membrane-associated Proteins." class="big_lightbox">

F0108). Quadrant markers were set based Goat IgG Control Antibody staining (Catalog #
AB-108-C, data not shown). View our protocol for
Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) Fas Ligand/TNFSF6 or (B) irrelevant protein, and eGFP were stained with Goat Anti-Human Fas Ligand/TNFSF6 Affinity Purified Polyclonal Antibody (Catalog # AF126) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
F0108). Quadrant markers were set based Goat IgG Control Antibody staining (Catalog #
AB-108-C, data not shown). View our protocol for
Staining Membrane-associated Proteins." class="big_thumb" />
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013)). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013)). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
MAB050), Recombinant Human Fas Ligand/TNFSF6 (Catalog #
126-FL) induces apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Apoptosis elicited by Recombinant Human Fas Ligand/TNFSF6 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Fas Ligand/TNFSF6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126). The ND
50 is typically 0.012-0.072 µg/mL." class="big_lightbox">

MAB050), Recombinant Human Fas Ligand/TNFSF6 (Catalog #
126-FL) induces apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Apoptosis elicited by Recombinant Human Fas Ligand/TNFSF6 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human Fas Ligand/TNFSF6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126). The ND
50 is typically 0.012-0.072 µg/mL." class="big_thumb" />
126-FL) was serially diluted and captured by Mouse Anti-Human Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog #
MAB126) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human Fas Ligand/TNFSF6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

126-FL) was serially diluted and captured by Mouse Anti-Human Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog #
MAB126) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human Fas Ligand/TNFSF6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />