Products Related to Gene-ID: 3558

1815-CL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-2 (Cys147Ser) (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815). The ND₅₀ is typically 0.25-1.5 µg/mL." class="big_lightbox"> 1815-CL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-2 (Cys147Ser) (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815). The ND₅₀ is typically 0.25-1.5 µg/mL." alt="Recombinant Canine IL-2 (Cys147Ser) (Catalog # 1815-CL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-2 (Cys147Ser) (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815). The ND₅₀ is typically 0.25-1.5 µg/mL." class="big_thumb" />

1815-CL) was serially diluted and captured by Goat Anti-Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1815-CL) was serially diluted and captured by Goat Anti-Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1815-CL) was serially diluted and captured by Goat Anti-Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1815-CL) was serially diluted and captured by Goat Anti-Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202R) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202). The ND₅₀ is typically 0.015-0.03 µg/mL." class="big_lightbox"> 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202). The ND₅₀ is typically 0.015-0.03 µg/mL." class="big_thumb" />

5476) for 5 hours or (B) resting were stained with Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202R) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Quadrant markers were set based on Mouse IgG1 isotype control antibody (MAB002) . To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_lightbox"> 5476) for 5 hours or (B) resting were stained with Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202R) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Quadrant markers were set based on Mouse IgG1 isotype control antibody (MAB002) . To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_thumb" />

PPK-002-GMP) stimulates proliferation of CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.0300-0.250 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of ProPak™ GMP IL-2." class="big_lightbox"> PPK-002-GMP) stimulates proliferation of CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.0300-0.250 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of ProPak™ GMP IL-2." alt="ProPak™ GMP-grade Recombinant Human IL-2 (PPK-002-GMP) stimulates proliferation of CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.0300-0.250 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of ProPak™ GMP IL-2." class="big_thumb" />

BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). After cytokine addition, the concentration of IL-2 (SPCKB-PS-000295) was determined by the Ella automated immunoassay system. Data is the average of 4 independent experiments with error bars ±SD. " class="big_lightbox"> BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). After cytokine addition, the concentration of IL-2 (SPCKB-PS-000295) was determined by the Ella automated immunoassay system. Data is the average of 4 independent experiments with error bars ±SD. " class="big_thumb" />

BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). T cells from 3 donors were activated and expanded in G-Rex bioreactors for 7 days and assessed for cells/cm² and cell viability. Data is the average of 4 independent experiments with 3 donors per experiment, error bars ±SD. " class="big_lightbox"> BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). T cells from 3 donors were activated and expanded in G-Rex bioreactors for 7 days and assessed for cells/cm² and cell viability. Data is the average of 4 independent experiments with 3 donors per experiment, error bars ±SD. " class="big_thumb" />

BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). T cells from 3 donors were activated for 1 day then transduced with eF1a-CD19-DHFR-eGFP lentivirus. Cells were expanded in a G-Rex® bioreactor for a total of 7 days, then assessed for fold expansion and cell viability. Data is the average of 3 donors, error bars ±SD. " class="big_lightbox"> BT-002-GMP) was added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). T cells from 3 donors were activated for 1 day then transduced with eF1a-CD19-DHFR-eGFP lentivirus. Cells were expanded in a G-Rex® bioreactor for a total of 7 days, then assessed for fold expansion and cell viability. Data is the average of 3 donors, error bars ±SD. " class="big_thumb" />

10453-IL) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is 0.05-0.3 ng/mL. Mammalian-expressed Recombinant Human IL-2 has similar activity to E.coli-derived Recombinant Human IL-2 (202-IL)." class="big_lightbox"> 10453-IL) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is 0.05-0.3 ng/mL. Mammalian-expressed Recombinant Human IL-2 has similar activity to E.coli-derived Recombinant Human IL-2 (202-IL)." class="big_thumb" />

10453-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa." class="big_lightbox"> 10453-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 14-18 kDa." class="big_thumb" />

BT-002-AFL) as the standard IL-2 protein or engineered Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days. The IL-2 (CD122-Directed) Protein supports greater than 6x more CD4+/CD8+ T cell growth than the standard IL-2 protein." class="big_lightbox"> BT-002-AFL) as the standard IL-2 protein or engineered Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days. The IL-2 (CD122-Directed) Protein supports greater than 6x more CD4+/CD8+ T cell growth than the standard IL-2 protein." alt="CD4⁺ and CD8⁺ T cells from two different donors were activated with anti-CD3-, anti-CD28-conjugated beads, and grown for 28 days in media containing either 3.6 or 14.3 ng/mL of Animal-free Recombinant Human IL-2 Protein (BT-002-AFL) as the standard IL-2 protein or engineered Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days. The IL-2 (CD122-Directed) Protein supports greater than 6x more CD4+/CD8+ T cell growth than the standard IL-2 protein." class="big_thumb" />

BT-002-AFL) as the standard IL-2 protein or Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. A) After 28 days, the phenotypes of the cells were compared by flow cytometry using a Brilliant Violet 711™ anti-human CD197 (CCR7) antibody and PE/Dazzle™ 594 anti-human CD45RA antibody to determine the number of CD4⁺ and CD8⁺ naive (CD45RA⁺/CCR7⁺) Tscm cells. The percentage of Tscm cells was found to be higher when the cells were expanded with the IL-2 (CD122-Directed) Protein at 14.3 ng/mL. B) The number of cells expressing the T cell exhaustion markers, PD-1, LAG-3, and TIM-3 was determined by flow cytometry. The results showed that the percentages of CD4⁺/CD8⁺ T cells that were also PD-1, LAG-3, or TIM-3 positive were equivalent or reduced with the IL-2 (CD122-Directed) Protein. These results demonstrate that the IL-2 (CD122-Directed) Protein promotes increased T cell expansion with enhanced expression of naive phenotype markers and equivalent or reduced expression of cell exhaustion markers. " class="big_lightbox"> BT-002-AFL) as the standard IL-2 protein or Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR), refreshed every 2-3 days. A) After 28 days, the phenotypes of the cells were compared by flow cytometry using a Brilliant Violet 711™ anti-human CD197 (CCR7) antibody and PE/Dazzle™ 594 anti-human CD45RA antibody to determine the number of CD4⁺ and CD8⁺ naive (CD45RA⁺/CCR7⁺) Tscm cells. The percentage of Tscm cells was found to be higher when the cells were expanded with the IL-2 (CD122-Directed) Protein at 14.3 ng/mL. B) The number of cells expressing the T cell exhaustion markers, PD-1, LAG-3, and TIM-3 was determined by flow cytometry. The results showed that the percentages of CD4⁺/CD8⁺ T cells that were also PD-1, LAG-3, or TIM-3 positive were equivalent or reduced with the IL-2 (CD122-Directed) Protein. These results demonstrate that the IL-2 (CD122-Directed) Protein promotes increased T cell expansion with enhanced expression of naive phenotype markers and equivalent or reduced expression of cell exhaustion markers. " class="big_thumb" />

10919-2B) was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR) was measured at a concentration range between 0.097nM and 200 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_d=49.3 nM. Note: The IL-2 (CD122-Directed) Protein was modeled to engage only the IL-2 dimeric beta gamma receptor complex. In comparison, wild type recombinant human IL-2 (BT-002) affinity to Recombinant Human IL 2 R beta Fc Chimera (10919-2B) has a binding constant K_d=544 nm." class="big_lightbox"> 10919-2B) was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human IL-2 (CD122-Directed) Protein (BT-002DBR) was measured at a concentration range between 0.097nM and 200 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_d=49.3 nM. Note: The IL-2 (CD122-Directed) Protein was modeled to engage only the IL-2 dimeric beta gamma receptor complex. In comparison, wild type recombinant human IL-2 (BT-002) affinity to Recombinant Human IL 2 R beta Fc Chimera (10919-2B) has a binding constant K_d=544 nm." class="big_thumb" />

BT-002-GMP) Animal-Free (Catalog # BT-002-AFL) and RUO (BT-002) grades of Recombinant Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively)." class="big_lightbox"> BT-002-GMP) Animal-Free (Catalog # BT-002-AFL) and RUO (BT-002) grades of Recombinant Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively)." class="big_thumb" />

402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑2 Polyclonal Antibody (Catalog # AB-402-NA). The ND₅₀ is typically 2-8 µg/mL." class="big_lightbox"> 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑2 Polyclonal Antibody (Catalog # AB-402-NA). The ND₅₀ is typically 2-8 µg/mL." alt="Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑2 Polyclonal Antibody (Catalog # AB-402-NA). The ND₅₀ is typically 2-8 µg/mL." class="big_thumb" />

HAF017). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of monensin treated human peripheral blood mononuclear cells (PBMCs) with no additioan treatment (-) or additionaly treated (+) with 0.5 ug/mL calcium ionomycin (Iono) and 50 ng/mL PMA overnight. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-2 Polyclonal Antibody (Catalog # AB-202-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 Polyclonal Antibody (Catalog # AB-202-NA). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox"> 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 Polyclonal Antibody (Catalog # AB-202-NA). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />