Products Related to Gene-ID: 3558

1020-RL) is coated at 1 µg/mL, Recombinant Biotinylated Human IL-2 (Catalog # BT202) binds with an ED₅₀ of 4-24 ng/mL." class="big_lightbox"> 1020-RL) is coated at 1 µg/mL, Recombinant Biotinylated Human IL-2 (Catalog # BT202) binds with an ED₅₀ of 4-24 ng/mL." alt="When Recombinant Human IL-2 Ra Fc Chimera (Catalog # 1020-RL) is coated at 1 µg/mL, Recombinant Biotinylated Human IL-2 (Catalog # BT202) binds with an ED₅₀ of 4-24 ng/mL." class="big_thumb" />

BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050)." class="big_lightbox"> BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050)." class="big_thumb" />

5476) for 5 hours were stained with (A) Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB10442) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by Anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> 5476) for 5 hours were stained with (A) Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB10442) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by Anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) treated with Cell Activation Cocktail 500x (Catalog # 5476) for 5 hours were stained with (A) Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB10442) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by Anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line) as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB10442). The ND₅₀ is typically 0.05-0.5 µg/mL." class="big_lightbox"> 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line) as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB10442). The ND₅₀ is typically 0.05-0.5 µg/mL." class="big_thumb" />

HAF018). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human PBMC conditioned media untreated (-) or treated (+) with 200mM PMA and Ionomycin 1uM for 24 hours. PVDF membrane was probed with 2.5 µg/mL of Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB104421) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Rabbit Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB10356) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

5476) for 5 hours were stained with (A) Rabbit Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB10356) or (B) Rabbit IgG isotype control antibody ((Catalog # MAB1050) followed by Anti-Rabbit IgG APC-conjugated Secondary Antibody (Catalog # F0111) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> 5476) for 5 hours were stained with (A) Rabbit Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB10356) or (B) Rabbit IgG isotype control antibody ((Catalog # MAB1050) followed by Anti-Rabbit IgG APC-conjugated Secondary Antibody (Catalog # F0111) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

BT-002-GMP), Animal-Free (BT-002-AFL) and RUO (Catalog # BT-002) grades of Recombinant Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively)." class="big_lightbox"> BT-002-GMP), Animal-Free (BT-002-AFL) and RUO (Catalog # BT-002) grades of Recombinant Human IL-2 as measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (orange, green, red, respectively)." class="big_thumb" />

579-R2) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-2 (Cys146Tyr) (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF579). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> 579-R2) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-2 (Cys146Tyr) (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF579). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Cotton Rat IL-2 (Cys146Tyr) (Catalog # 579-R2) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-2 (Cys146Tyr) (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF579). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />