Products Related to Gene-ID: 3558

HAF017). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of monensin treated human peripheral blood mononuclear cells (PBMCs) with no additional treatment (-) or additionally treated (+) with 0.5 μg/mL calcium ionomycin (Iono) and 50 ng/mL PMA overnight. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-202-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-2 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line) as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-202-NA). The ND₅₀ is typically ≤ 0.15 µg/mL." class="big_lightbox"> 202-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line) as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human IL-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-202-NA). The ND₅₀ is typically ≤ 0.15 µg/mL." class="big_thumb" />

202-IL) was serially diluted and captured by Mouse Anti-Human/Primate IL‑2 Monoclonal Antibody (Catalog # MAB602) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-202-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 202-IL) was serially diluted and captured by Mouse Anti-Human/Primate IL‑2 Monoclonal Antibody (Catalog # MAB602) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-202-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Feline IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1890-FL) induces cell proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Feline IL-2 (Cys146Ser) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890). The ND₅₀ is typically 0.03-0.15 µg/mL." class="big_lightbox"> 1890-FL) induces cell proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Feline IL-2 (Cys146Ser) (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890). The ND₅₀ is typically 0.03-0.15 µg/mL." class="big_thumb" />

1890-FL) was serially diluted and captured by Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1890-FL) was serially diluted and captured by Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1890-FL) was serially diluted and captured by Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1890-FL) was serially diluted and captured by Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1890) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

BT-002HS) Provides consistent CD4+/CD8+ T Cell Proliferation in Side-by-Side Testing with the Standard IL-2 Protein. CD4+ and CD8+ T cells were activated with anti-CD3- , anti-CD28- conjugated beads, and grown for 14 days in media containing 10 ng/mL Animal-free Recombinant Human IL-2 (R&D Systems, Catalog # BT-002-AFL) as the standard IL-2 protein or the AI modified Heat Stable IL-2. IL-2 conditions were refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days and showed that the Heat Stable IL-2 protein performs equally compared to the standard IL-2 protein." class="big_lightbox"> BT-002HS) Provides consistent CD4+/CD8+ T Cell Proliferation in Side-by-Side Testing with the Standard IL-2 Protein. CD4+ and CD8+ T cells were activated with anti-CD3- , anti-CD28- conjugated beads, and grown for 14 days in media containing 10 ng/mL Animal-free Recombinant Human IL-2 (R&D Systems, Catalog # BT-002-AFL) as the standard IL-2 protein or the AI modified Heat Stable IL-2. IL-2 conditions were refreshed every 2-3 days. The average fold expansion of the cells was determined every 2-3 days and showed that the Heat Stable IL-2 protein performs equally compared to the standard IL-2 protein." class="big_thumb" />

BT-002-AFL) as the standard IL-2 protein or Heat Stable IL-2 (Cat # BT-002HS). IL-2 conditions were refreshed every 2-3 days. After 21 days, the phenotypes of the cells were compared by flow cytometry using a PE-Cy7-conjugated mouse anti-human CD45RA monoclonal antibody and a Brilliant Violet 711 conjugated mouse anti-human CCR7 monoclonal antibody to determine the number of CD4+ and CD8+ naive (CD45RA+ CCR7+), central memory (CD45RA- CCR7+), terminal effector memory (CD45RA+ CCR7- ), and effector memory (CD45RA- CCR7- ) T cells. The percentages of each of these cell populations were found to be similar whether the cells were expanded with the standard or Heat Stable IL-2 protein." class="big_lightbox"> BT-002-AFL) as the standard IL-2 protein or Heat Stable IL-2 (Cat # BT-002HS). IL-2 conditions were refreshed every 2-3 days. After 21 days, the phenotypes of the cells were compared by flow cytometry using a PE-Cy7-conjugated mouse anti-human CD45RA monoclonal antibody and a Brilliant Violet 711 conjugated mouse anti-human CCR7 monoclonal antibody to determine the number of CD4+ and CD8+ naive (CD45RA+ CCR7+), central memory (CD45RA- CCR7+), terminal effector memory (CD45RA+ CCR7- ), and effector memory (CD45RA- CCR7- ) T cells. The percentages of each of these cell populations were found to be similar whether the cells were expanded with the standard or Heat Stable IL-2 protein." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-2 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

2465-BL) was serially diluted and captured by Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2465-BL) was serially diluted and captured by Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2465-BL) was serially diluted and captured by Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2465-BL) was serially diluted and captured by Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Bovine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1613-IL) was serially diluted and captured by Goat Anti-Equine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1613-IL) was serially diluted and captured by Goat Anti-Equine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1613-IL) was serially diluted and captured by Goat Anti-Equine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1613-IL) was serially diluted and captured by Goat Anti-Equine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1613) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-402-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_lightbox"> 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-402-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." alt="Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-402-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_thumb" />

402-ML) was serially diluted and captured by Rat Anti-Mouse IL‑2 Monoclonal Antibody (Catalog # MAB702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-402-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 402-ML) was serially diluted and captured by Rat Anti-Mouse IL‑2 Monoclonal Antibody (Catalog # MAB702) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-402-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

652-P2)." class="big_lightbox"> 652-P2)." alt="Porcine IL-2 Antibody Antigen Affinity-purified Polyclonal Goat IgG Antibody (Catalog # AF652) neutralizes IL 2-induced proliferation in the CTLL 2 mouse cytotoxic T cell line. The Neutralization Dose (ND₅₀) is typically 0.0500-0.600 µg/mL in the presence of 2 ng/mL Recombinant Porcine IL 2 (652-P2)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />