| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF774-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB244U-100UG | |||
| Alexa Fluor 405 | FAB244V-100UG | |||
| Alexa Fluor 488 | FAB244G-100UG | |||
| Alexa Fluor 532 | FAB244X-100UG | |||
| Alexa Fluor 594 | FAB244T-100UG | |||
| Alexa Fluor 647 | FAB244R-100UG | |||
| Alexa Fluor 700 | FAB244N-100UG | |||
| Alexa Fluor 750 | FAB244S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 774-M2-200 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 803-I2-200 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-66624-0.025mg | |||
![ICAM-2 WT and variants co-precipitated with alpha -actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha -actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha -actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha -actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha -actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af244_human-icam-2-cd102-affinity-purified-polyclonal-ab-8220249503222.jpg "ICAM-2 WT and variants co-precipitated with alpha -actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha -actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha -actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha -actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha -actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.")
![ICAM-2 WT and variants co-precipitated with alpha -actinin. A) IP/IB experiments with control cell lines demonstrate the expected association of ICAM-2, alpha -actinin and actin in lysates from SK-N-ASpIRES.ICAM-2 cells (labeled as WT), but not SK-N-ASpIRESneo cells (neo). Results for these control cell lines were published previously [13]. B) ICAM-2 glycosylation site variants associated simultaneously with alpha -actinin and actin. Immunoprecipitations (IP) were performed using whole cell lysates and a mouse monoclonal antibody to alpha -actinin (MAB1682, Millipore). Following protein separation by electrophoresis, immunoblots (IB) were performed with antibodies to alpha -actinin (sc-7454, Santa Cruz Biotech), ICAM-2 (AF244, R&D Systems), or actin (4968, Cell Signaling). The presence of ICAM-2 WT and variants in each preparation was confirmed by immunoblot analysis of input preparations (a representative blot is shown in Figure 2C) and also by immunoblot analysis of the proteins remaining in the supernatant following precipitation (not shown), to confirm the presence of sufficient/excess ICAM-2 protein or variant in each preparation used for immunoprecipitation experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23714211), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af244_human-icam-2-cd102-affinity-purified-polyclonal-ab-44202415425087.jpg)
![Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32456_rabbit-icam-2-cd102-mab-je63-28-278202416285280.jpg "Western Blot: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Western blot analysis of ICAM-2/CD102 on different lysates with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse kidney tissue lysate Lane 5: Mouse spleen tissue lysate (no heat) Lane 6: Rat spleen tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 31 kDa Observed band size: 45-50 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32456) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ICAM-2/CD102 Antibody (JE63-28) [NBP3-32456] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-ICAM-2/CD102 antibody (NBP3-32456) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32456) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32456_rabbit-icam-2-cd102-mab-je63-28-298202414262629.jpg)
![Western Blot: ICAM-2/CD102 Antibody [NBP1-31612] - Sample(30 ug whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Western-Blot-NBP1-31612-img0005.jpg "Western Blot: ICAM-2/CD102 Antibody [NBP1-31612] - Sample(30 ug whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ICAM-2/CD102 Antibody [NBP1-31612] - Paraffin-embedded breast cancer, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Immunohistochemistry-Paraffin-NBP1-31612-img0004.jpg)
![Western Blot: ICAM-2/CD102 Antibody [NBP2-16912] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Western-Blot-NBP2-16912-img0003.jpg "Western Blot: ICAM-2/CD102 Antibody [NBP2-16912] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE gel, diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: ICAM-2/CD102 Antibody [NBP2-16912] - K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ICAM2 protein stained by ICAM2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16912-img0002.jpg)
![Western Blot: ICAM-2/CD102 Antibody [NBP2-32236] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-2 antibody NBP2-32236 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ICAM-2-CD102-Antibody-Western-Blot-NBP2-32236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ICAM-2/CD102 Antibody [NBP2-32236] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-ICAM-2 antibody NBP2-32236 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-08994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.](http://images.novusbio.com/fullsize/ICAM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08994-img0001.jpg "Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-08994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.")
![Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-09322] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.](http://images.novusbio.com/fullsize/ICAM2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09322-img0001.jpg "Western Blot: ICAM2 Overexpression Lysate (Adult Normal) [NBP2-09322] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM2.")