| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87109-25ul | |||
HAF018). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Histone Deacetylase 2/HDAC2 Monoclonal Antibody (Catalog # MAB7679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7679-SP | |||
HAF016). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Rat-2 rat embryonic fibroblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat Histone Deacetylase 2/HDAC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7679) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Histone Deacetylase 2/HDAC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7679-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41285-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26239-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52479-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26238-50ul | |||
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Analysis in mouse cell line NIH-3T3.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Western-Blot-NBP1-87109-img0014.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Analysis in mouse cell line NIH-3T3.")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87109-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-87109-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Western-Blot-NBP1-87109-img0008.jpg)
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-87109-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-87109-img0011.jpg)
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody [NBP1-87109] - Staining of human prostate shows moderate nuclear positivity in smooth muscle cells and glandular cells.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Immunohistochemistry-Paraffin-NBP1-87109-img0012.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts from HeLa cells (25 ug) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-C-15200201-Western-Blot-NBP2-59269-img0002.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts from HeLa cells (25 ug) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-C-15200201-Immunofluorescence-NBP2-59269-img0004.jpg)
![Chromatin Immunoprecipitation: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - ChIP assays were performed using human HeLa cells, the antibody consisting of 1, 2 and 5 ug per ChIP experiment was analyzed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the ACTB, EIF4A2 and LMO4 genes, used as positive controls, and for the Sat2 satellite repeat, used as negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-C-15200201-Chromatin-Immunoprecipitation-NBP2-59269-img0003.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts (40 ug) from HeLa cells transfected with HDAC2 siRNA (lane 2) and from an untransfected control (lane 1) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-C-15200201-Western-Blot-NBP2-59269-img0001.jpg)
![Western Blot: HDAC2 Antibody [NBP2-16790] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/HDAC2-Antibody-Western-Blot-NBP2-16790-img0001.jpg "Western Blot: HDAC2 Antibody [NBP2-16790] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody [NBP2-16790] - HDAC2 antibody detects HDAC2 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. HDAC2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-16790-img0004.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP2-41285] - Western blot analysis of HDAC2 in HeLa cell lysate with HDAC2 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Western-Blot-NBP2-41285-img0001.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP2-41285] - Western blot analysis of HDAC2 in HeLa cell lysate with HDAC2 antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody [NBP2-41285] - Immunohistochemistry of HDAC2 in rat testis tissue with HDAC2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Immunohistochemistry-NBP2-41285-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody - BSA Free [NBP2-41285] - Immunofluorescence of Histone Deacetylase 2/HDAC2 in rat testis tissue with Histone Deacetylase 2/HDAC2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41285_rabbit-polyclonal-histone-deacetylase-2-hdac2-antibody-271120242013246.jpg)
![Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26239_rabbit-histone-deacetylase-2-hdac2-mab-9h4-262202414471555.jpg "Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Image of Histone Deacetylase 2/HDAC2 Antibody (9H4) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26239_rabbit-histone-deacetylase-2-hdac2-mab-9h4-2722024847191.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Positive Western Blot detected in: MCF-7 whole cell lysate, K562 whole cell lysate.<br /> All lanes: Histone Deacetylase 2/HDAC2 Antibody at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 56, 52 kDa<br /> Observed band size: 60 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26239_rabbit-histone-deacetylase-2-hdac2-mab-9h4-282202411442634.jpg)
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (9H4) [NBP3-26239] - Staining of Hela Cells with Histone Deacetylase 2/HDAC2 Antibody (9H4) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26239_rabbit-histone-deacetylase-2-hdac2-mab-9h4-2822024132366.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-44095] - Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC2 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 59 kDa for HDAC2.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-Western-Blot-NBP1-44095-img0003.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody [NBP1-44095] - Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC2 antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 59 kDa for HDAC2.")
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893] - Western blot detection of Histone Deacetylase 2/HDAC2 in K562, C6, 3T3 cell lysates using Histone Deacetylase 2/HDAC2 Rabbit mAb (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 60kDa.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-S06-4E4-Western-Blot-NBP3-14893-img0001.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893] - Western blot detection of Histone Deacetylase 2/HDAC2 in K562, C6, 3T3 cell lysates using Histone Deacetylase 2/HDAC2 Rabbit mAb (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 60kDa.")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893] - Immunofluorescence of Histone Deacetylase 2/HDAC2 (green) in Hela cells using Histone Deacetylase 2/HDAC2 Rabbit mAb at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-S06-4E4-Immunocytochemistry-Immunofluorescence-NBP3-14893-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (S06-4E4) [NBP3-14893] - Immunohistochemistry of Histone Deacetylase 2/HDAC2 in paraffin-embedded Human colon cancer tissue using Histone Deacetylase 2/HDAC2 Rabbit mAb at dilution 1/1](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-S06-4E4-Immunohistochemistry-Paraffin-NBP3-14893-img0003.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Western blot analysis of extracts of various cell lines, using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Western-Blot-NBP3-15824-img0006.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Western blot analysis of extracts of various cell lines, using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunoprecipitation: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Histone Deacetylase 2/HDAC2 antibody (NBP3-15824). Western blot was performed from the immunoprecipitate using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-10R7O1-Immunoprecipitation-NBP3-15824-img0007.jpg)
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (4C10C3) [NBP2-52479] - Analysis using HDAC2 mAb against human HDAC2 (AA: 217-327) recombinant protein. (Expected MW is 38.1 kDa)](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-4C10C3-Western-Blot-NBP2-52479-img0001.jpg "Western Blot: Histone Deacetylase 2/HDAC2 Antibody (4C10C3) [NBP2-52479] - Analysis using HDAC2 mAb against human HDAC2 (AA: 217-327) recombinant protein. (Expected MW is 38.1 kDa)")
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (4C10C3) [NBP2-52479] - Analysis using HDAC2 mAb against HEK293 (1) and HDAC2 (AA: 217-327)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-4C10C3-Western-Blot-NBP2-52479-img0002.jpg)
![ELISA: Histone Deacetylase 2/HDAC2 Antibody (4C10C3) [NBP2-52479] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Histone-Deacetylase-2-HDAC2-Antibody-4C10C3-ELISA-NBP2-52479-img0003.jpg)
![Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9E9) [NBP3-26238] - Image of Histone Deacetylase 2/HDAC2 Antibody (9E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26238_rabbit-histone-deacetylase-2-hdac2-mab-9e9-27220248313710.jpg "Immunohistochemistry: Histone Deacetylase 2/HDAC2 Antibody (9E9) [NBP3-26238] - Image of Histone Deacetylase 2/HDAC2 Antibody (9E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Histone Deacetylase 2/HDAC2 Antibody (9E9) [NBP3-26238] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate.<br /> All lanes: Histone Deacetylase 2/HDAC2 Antibody at 1: 2000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 56, 52 kDa<br /> Observed band size: 60 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26238_rabbit-histone-deacetylase-2-hdac2-mab-9e9-28220241140918.jpg)
