| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35521-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22636-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57280-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58120-25ul | |||
![Western Blot: TFIIE-alpha Antibody [NBP1-46196] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TFIIE-alpha antibody NBP1-46196 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TFIIE-alpha was also immunoprecipitated by rabbit anti-TFIIE-alpha antibody NBP1-46195, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Western-Blot-NBP1-46196-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TFIIE-alpha Antibody [NBP1-46196] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TFIIE-alpha antibody NBP1-46196 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TFIIE-alpha was also immunoprecipitated by rabbit anti-TFIIE-alpha antibody NBP1-46195, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46196] - Section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- GTF2E1/TFIIE-alpha used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-46196-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: TFIIE-alpha Antibody [NBP3-35521] - Immunofluorescence analysis of U-2 OS cells using TFIIE-alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35521_rabbit-tfiie-alpha-pab-101220241214469.jpg "Immunocytochemistry/ Immunofluorescence: TFIIE-alpha Antibody [NBP3-35521] - Immunofluorescence analysis of U-2 OS cells using TFIIE-alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: TFIIE-alpha Antibody [NBP3-35521] - Western blot analysis of various lysates using TFIIE-alpha Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5S.](http://images.novusbio.com/fullsize/nbp3-35521_rabbit-tfiie-alpha-pab-1012202411531035.jpg)
![Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46195] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- GTF2E1/TFIIE-alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Immunohistochemistry-NBP1-46195-img0003.jpg "Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46195] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- GTF2E1/TFIIE-alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: TFIIE-alpha Antibody [NBP1-46195] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha antibody used for IP at 3 mcg/mg lysate. GTF2E1/TFIIE-alpha was also immunoprecipitated by rabbit anti-GTF2E1/TFIIE-alpha antibody NBP1-46196, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Immunoprecipitation-NBP1-46195-img0002.jpg)
![Western Blot: TFIIE-alpha Antibody (SR2046) [NBP3-22636] - Western blot analysis using NBP3-22636 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22636_rabbit-tfiie-alpha-mab-sr2046-5720231564716.jpg "Western Blot: TFIIE-alpha Antibody (SR2046) [NBP3-22636] - Western blot analysis using NBP3-22636 in HeLa cell lysate.")
![Western Blot: TFIIE-alpha Antibody [H00002960-B01P] - Analysis of GTF2E1 expression in transfected 293T cell line by GTF2E1 polyclonal antibody. Lane 1: GTF2E1 transfected lysate(48.29 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Western-Blot-H00002960-B01P-img0002.jpg "Western Blot: TFIIE-alpha Antibody [H00002960-B01P] - Analysis of GTF2E1 expression in transfected 293T cell line by GTF2E1 polyclonal antibody. Lane 1: GTF2E1 transfected lysate(48.29 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: TFIIE-alpha Antibody [H00002960-B01P] - Analysis of purified antibody to GTF2E1 on HepG2 cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Immunocytochemistry-Immunofluorescence-H00002960-B01P-img0001.jpg)
![Western Blot: TFIIE-alpha Antibody [H00002960-D01P] - Analysis of GTF2E1 expression in transfected 293T cell line by GTF2E1 polyclonal antibody.Lane 1: GTF2E1 transfected lysate(49.50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Western-Blot-H00002960-D01P-img0002.jpg "Western Blot: TFIIE-alpha Antibody [H00002960-D01P] - Analysis of GTF2E1 expression in transfected 293T cell line by GTF2E1 polyclonal antibody.Lane 1: GTF2E1 transfected lysate(49.50 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: TFIIE-alpha Antibody [H00002960-D01P] - Analysis of antibody to GTF2E1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Chromatin-Immunoprecipitation-H00002960-D01P-img0003.jpg)
![Immunohistochemistry: TFIIE-alpha Antibody [NBP3-10290] - Immunohistochemical analysis of human muscle.](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Immunohistochemistry-NBP3-10290-img0001.jpg "Immunohistochemistry: TFIIE-alpha Antibody [NBP3-10290] - Immunohistochemical analysis of human muscle.")
![Western Blot: TFIIE-alpha Antibody (JE66-41) [NBP3-33006] - Western blot analysis of TFIIE-alpha on different lysates with Rabbit anti-TFIIE-alpha antibody (NBP3-33006) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 ug/Lane) Lane 2: Jurkat cell lysate (15 ug/Lane) Lane 3: Daudi cell lysate (15 ug/Lane) Lane 4: HeLa cell lysate (15 ug/Lane) Lane 5: A431 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: HepG2 cell lysate (15 ug/Lane) Lane 8: HEK-293 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: MCF7 cell lysate (15 ug/Lane) Lane 11: Mouse testis tissue lysate (30 ug/Lane) Lane 12: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 49.5 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33006) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33006_rabbit-tfiie-alpha-mab-je66-41-288202412493492.jpg "Western Blot: TFIIE-alpha Antibody (JE66-41) [NBP3-33006] - Western blot analysis of TFIIE-alpha on different lysates with Rabbit anti-TFIIE-alpha antibody (NBP3-33006) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 ug/Lane) Lane 2: Jurkat cell lysate (15 ug/Lane) Lane 3: Daudi cell lysate (15 ug/Lane) Lane 4: HeLa cell lysate (15 ug/Lane) Lane 5: A431 cell lysate (15 ug/Lane) Lane 6: A549 cell lysate (15 ug/Lane) Lane 7: HepG2 cell lysate (15 ug/Lane) Lane 8: HEK-293 cell lysate (15 ug/Lane) Lane 9: NIH/3T3 cell lysate (15 ug/Lane) Lane 10: MCF7 cell lysate (15 ug/Lane) Lane 11: Mouse testis tissue lysate (30 ug/Lane) Lane 12: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 49.5 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33006) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: TFIIE-alpha Antibody [NBP2-57280] - Staining of human cell line CACO-2 shows localization to nucleus.](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57280-img0001.jpg "Immunocytochemistry/Immunofluorescence: TFIIE-alpha Antibody [NBP2-57280] - Staining of human cell line CACO-2 shows localization to nucleus.")
![Immunocytochemistry/Immunofluorescence: TFIIE-alpha Antibody [NBP2-58120] - Staining of human cell line PC-3 shows localization to nucleus.](http://images.novusbio.com/fullsize/TFIIE-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58120-img0001.jpg "Immunocytochemistry/Immunofluorescence: TFIIE-alpha Antibody [NBP2-58120] - Staining of human cell line PC-3 shows localization to nucleus.")
![Western Blot: TFIIE-alpha Overexpression Lysate (Adult Normal) [NBP2-10463] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TFIIE-alpha.](http://images.novusbio.com/fullsize/TFIIE-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10463-img0001.jpg "Western Blot: TFIIE-alpha Overexpression Lysate (Adult Normal) [NBP2-10463] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TFIIE-alpha.")