| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-3638SS | |||
| Azide and BSA Free | NBP2-80568 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB59051-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5905-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-49646AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-49646AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-49646AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-49646AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-49646AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48110 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47993-0.1mg | |||
| IHC-Prediluted | NBP2-48110 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5905-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34056-25ul | |||
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunocytochemistry-Immunofluorescence-NB110-3638-img0005.jpg "Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Representative montage images are shown for control dissociated DRG culture. (B) Dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs. Image collected and cropped by CiteAb from the following publication (eneuro.org/content/4/1/ENEURO.0171-16.2017), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunocytochemistry-Immunofluorescence-NB110-3638-img0003.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Western-Blot-NB110-3638-img0002.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Alkaline Phosphatase, Placental Antibody (8B6) [NB110-3638] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic Induced Epitope Retrieval (PIER) was used.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Immunohistochemistry-Paraffin-NB110-3638-img0001.jpg)
![Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) [NB110-3638] - Detection of Alkaline Phosphatase/ALPP in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # NB110-3638, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-8B6-Flow-Cytometry-NB110-3638-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - GRPs enhance axonal growth in DRGs in vitro. A–C, Representative montage images are shown for control dissociated DRG culture (A), dissociated DRGs cocultured with rat GRPs (B), & dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs (C). beta III-tubulin (red) immunofluorescence highlights the neurons; GRPs are visualized by immunostaining for alkaline phosphatase (green). D shows quantification for the average length of the longest axon per neuron ± SEM (n ≥ 30 neurons in three separate experiments; **p ≤ 0.01 by Student’s t test). Scale bar, 250 μm. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202415371956.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - GRPs enhance axonal growth in DRGs in vitro. A–C, Representative montage images are shown for control dissociated DRG culture (A), dissociated DRGs cocultured with rat GRPs (B), & dissociated DRGs cultures exposed to conditioned medium from alkaline phosphatase-expressing rat GRPs (C). beta III-tubulin (red) immunofluorescence highlights the neurons; GRPs are visualized by immunostaining for alkaline phosphatase (green). D shows quantification for the average length of the longest axon per neuron ± SEM (n ≥ 30 neurons in three separate experiments; **p ≤ 0.01 by Student’s t test). Scale bar, 250 μm. Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202415175250.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202416235514.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-310202416284919.jpg)
![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - BSA Free [NB110-3638] - Coculture with neural & glial progenitor cells increases axonal outgrowth from adult DRG neurons. A–C, Representative montage images of control-dissociated DRG cultures (A), dissociated DRGs cocultured with rat GRPs/NRPs (B), & dissociated DRG cultures exposed to conditioned medium from parallel rat GRP/NRP cultures (C) are shown. Immunofluorescence for beta III-tubulin (red) & nestin (green) highlight DRG neurons & GRP/NRP nuclei, respectively. D, Quantitation of the average lengths of the longest axon per neuron (±SEM) for the above conditions is shown. Coculture with GRPs/NRPs significantly increases in axon length compared with the standard DRG culture; exposure to conditioned medium from GRP/NRP cultures showed a further increase in axon length (n ≥ 30 neurons in three separate experiments; *p ≤ 0.05 & ***p ≤ 0.001 by Student’s t test). Scale bar, 250 μm. E, Quantitation of axon growth parameters for 7 d injury-conditioned DRG neurons cultured on coverslips laid over a bed of GRP/NRP cells (coculture) or control conditions is shown (n ≥ 200 neurons analyzed in three separate experiments; p values represent ANOVA with Tukey post hoc analyses). Image collected & cropped by CiteAb from the following publication (//www.eneuro.org/lookup/doi/10.1523/ENEURO.0171-16.2017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-3638_mouse-monoclonal-alkaline-phosphatase-alpp-antibody-8b6-31020241628494.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of Placental alkaline phosphatase/ALPP on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase/ALPP antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Western-Blot-NBP2-67136-img0005.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of Placental alkaline phosphatase/ALPP on human placenta tissue lysates with Rabbit anti-Placental alkaline phosphatase/ALPP antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Staining PLAP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunocytochemistry-Immunofluorescence-NBP2-67136-img0002.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human seminoma tissue with Rabbit anti-Placental alkaline phosphatase/ALPP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0007.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Western-Blot-NBP2-67136-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Staining PLAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunocytochemistry-Immunofluorescence-NBP2-67136-img0001.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human placenta tissue using anti-PLAP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0003.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (JM22-53) [NBP2-67136] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Placental alkaline phosphatase/ALPP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-JM22-53-Immunohistochemistry-Paraffin-NBP2-67136-img0006.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993] - Western Blot Analysis of JEG-3 cell lysate using PLAP MAb (PL8-F6).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-PL8-F6-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47993-img0002.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993] - Western Blot Analysis of JEG-3 cell lysate using PLAP MAb (PL8-F6).")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993] - Human Placenta stained with PLAP Monoclonal Antibody (PL8-F6).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-PL8-F6-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47993-img0001.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6) [NBP2-44959] - Western Blot Analysis of JEG-3 cell lysate using PLAP MAb (PL8-F6). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-PL8-F6-Western-Blot-NBP2-44959-img0002.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6) [NBP2-44959] - Western Blot Analysis of JEG-3 cell lysate using PLAP MAb (PL8-F6). Image using the Azide and BSA Free format of this antibody.")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (PL8-F6) [NBP2-44959] - Human Placenta stained with PLAP Monoclonal Antibody (PL8-F6).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-PL8-F6-Immunohistochemistry-Paraffin-NBP2-44959-img0001.jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody [NBP2-34056] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15,10. Lane 2: Placenta](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-Western-Blot-NBP2-34056-img0005.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody [NBP2-34056] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15,10. Lane 2: Placenta")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody [NBP2-34056] - Staining of human cell line HeLa shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34056-img0006.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody [NBP2-34056] - Staining in human placenta shows strong cytoplasmic and membranous staining in trophoblasts.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-Immunohistochemistry-Paraffin-NBP2-34056-img0004.jpg)
![Western Blot: Alkaline Phosphatase/ALPP/ALPI Antibody (10Q2X7) [NBP3-16373] - Western blot analysis of extracts of PC-3 cells, using Alkaline Phosphatase/ALPP/ALPI Rabbit mAb (NBP3-16373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-ALPI-Antibody-10Q2X7-Western-Blot-NBP3-16373-img0004.jpg "Western Blot: Alkaline Phosphatase/ALPP/ALPI Antibody (10Q2X7) [NBP3-16373] - Western blot analysis of extracts of PC-3 cells, using Alkaline Phosphatase/ALPP/ALPI Rabbit mAb (NBP3-16373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Alkaline Phosphatase/ALPP/ALPI Antibody (10Q2X7) [NBP3-16373] - Western blot analysis of extracts of Mouse testis, using Alkaline Phosphatase/ALPP/ALPI Rabbit mAb (NBP3-16373) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-ALPI-Antibody-10Q2X7-Western-Blot-NBP3-16373-img0005.jpg)
![Alkaline Phosphatase/ALPP/ALPI Antibody (10Q2X7) [NBP3-16373] - Human placenta using Placental alkaline phosphatase (PLAP) antibody at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16373_rabbit-alkaline-phosphatase-alpp-alpi-mab-10q2x7-12520239656..jpg)
![Western Blot: Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) - Azide and BSA Free [NBP3-08817] - Western Blot Analysis of JEG-3 cell lysate using Alkaline Phosphatase/ALPP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-ALPP-2899R-Azide-and-BSA-Free-Western-Blot-NBP3-08817-img0002.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) - Azide and BSA Free [NBP3-08817] - Western Blot Analysis of JEG-3 cell lysate using Alkaline Phosphatase/ALPP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (ALPP/2899R) - Azide and BSA Free [NBP3-08817] - Formalin-fixed, paraffin-embedded human placenta stained with Alkaline Phosphatase/ALPP Rabbit Recombinant Monoclonal Antibody (ALPP/2899R).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-ALPP-Antibody-ALPP-2899R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08817-img0001.jpg)
