| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49644SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26261-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26260-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82941-25ul | |||
![Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP1-49644-img0001.jpg "Western Blot: USP22 Antibody [NBP1-49644] - Western blot analysis of Usp22 in HeLa whole cell extracts.")
![Western Blot: USP22 Antibody [NBP1-49644] - analysis of USP22 in HeLa whole cell lysate using anti-USP22 antibody. The primary antibody was used at a dilution of 1:500 and incubated overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP1-49644-img0002.jpg)
![Simple Western: USP22 Antibody [NBP1-49644] - Simple Western lane view shows a specific band for USP22 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/USP22-Antibody-Simple-Western-NBP1-49644-img0003.jpg)
![Western Blot: USP22 Antibody (18H6) [NBP3-26261] - Positive Western Blot detected in: A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate. All lanes: USP22 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 59 kDa Observed band size: 55-72 kDa](http://images.novusbio.com/fullsize/nbp3-26261_rabbit-usp22-mab-18h6-282202411472117.jpg "Western Blot: USP22 Antibody (18H6) [NBP3-26261] - Positive Western Blot detected in: A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate. All lanes: USP22 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 59 kDa Observed band size: 55-72 kDa")
![Immunocytochemistry/Immunofluorescence: USP22 Antibody (18H6) [NBP3-26261] - Staining of Hela cell with USP22 Antibody (18H6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 507-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26261_rabbit-usp22-mab-18h6-282202412475133.jpg)
![Flow Cytometry: USP22 Antibody (18H6) [NBP3-26261] - Overlay Peak curve showing Hela cells stained with USP22 Antibody (18H6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26261_rabbit-usp22-mab-18h6-282202414312718.jpg)
![Western Blot: USP22 Antibody (9C7I8) [NBP3-16794] - Western blot analysis of extracts of Mouse thymus, using USP22 Rabbit mAb (NBP3-16794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/USP22-Antibody-9C7I8-Western-Blot-NBP3-16794-img0002.jpg "Western Blot: USP22 Antibody (9C7I8) [NBP3-16794] - Western blot analysis of extracts of Mouse thymus, using USP22 Rabbit mAb (NBP3-16794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: USP22 Antibody (9C7I8) [NBP3-16794] - analysis of extracts of HepG2 cells, using USP22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16794_rabbit-usp22-mab-9c7i8-western-blot-3112023125123..jpg)
![Western Blot: USP22 Antibody (9C7I8) [NBP3-16794] - Western blot analysis of lysates from Rat lung, using USP22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-16794_rabbit-usp22-mab-9c7i8-522025121543.jpg)
![Western Blot: USP22 Antibody [NBP2-20829] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/USP22-Antibody-Western-Blot-NBP2-20829-img0001.jpg "Western Blot: USP22 Antibody [NBP2-20829] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunoprecipitation: USP22 Antibody [NBP2-20829] - Immunoprecipitation of USP22 protein from A549 whole cell extracts using 5 ug of USP22 antibody [N3C3]. Western blot analysis was performed using USP22 antibody [N3C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/USP22-Antibody-Immunoprecipitation-NBP2-20829-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: USP22 Antibody (18E3) [NBP3-26260] - Staining of MCF-7 with USP22 Antibody (18E3) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 493-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26260_rabbit-usp22-mab-18e3-282202412475145.jpg "Immunocytochemistry/Immunofluorescence: USP22 Antibody (18E3) [NBP3-26260] - Staining of MCF-7 with USP22 Antibody (18E3) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 493-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: USP22 Antibody (18E3) [NBP3-26260] - Overlay Peak curve showing Hela cells stained with USP22 Antibody (18E3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26260_rabbit-usp22-mab-18e3-282202414183656.jpg)
![Western Blot: USP22 Overexpression Lysate (Adult Normal) [NBL1-17653] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP22.](http://images.novusbio.com/fullsize/USP22-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17653-img0002.jpg "Western Blot: USP22 Overexpression Lysate (Adult Normal) [NBL1-17653] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP22.")
![Immunocytochemistry/Immunofluorescence: USP22 Antibody [NBP1-82941] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/USP22-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82941-img0008.jpg "Immunocytochemistry/Immunofluorescence: USP22 Antibody [NBP1-82941] - Staining of human cell line U-251 MG shows localization to nuclear speckles. Antibody staining is shown in green.")
